Transgenic zebrafish model with macrophages aggregated specifically in liver

Abstract

The invention discloses a transgenic zebrafish model with macrophages aggregated specifically in liver. The zebrafish refers to the Tg (fabp10a: il34) transgenic zebrafish. The transgenic zebrafish model with macrophages aggregated specifically in liver is realized by the following steps: (1) zebrafish culture; (2) whole-mount in situ hybridization and antibody staining of zebrafish embryos; and (3) laser scanning confocal fluorescence microscopy in vivo imaging. The transgenic zebrafish model with macrophages aggregated specifically in liver proves for the first time that heterotopically expressed and overexpressed interleukin 34 in the liver can induce the macrophages to enrich into the liver, and provides solid and direct evidence by using the in vivo imaging technology. In addition, the Tg (fabp10a: il34; mpeg1: GFP) adult zebrafish has the following phenotypes: (1) the number of the macrophages increases only specifically in the liver region; (2) the number of the macrophages remains unchanged in other tissue regions such as tail hematopoietic tissue (CHT); and (3) the number of granulocytes is unchanged.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a transgenic zebrafish model for liver-specific accumulation of macrophages. Background technique [0002] Macrophages are a type of myeloid cell and an important part of the immune system, playing a key role in clearing the organism of foreign material, including cellular debris, microbes and cancer cells. In adult mammals, these large phagocytes are present in all tissues in which they are immunosurveilled and are able to engulf and destroy damaged tissue, helping to initiate the healing process. Macrophages can be divided into several types such as Kupffer cells, microglia, and Langerhans cells according to their location in different tissues, but in general, they are all part of the mononuclear phagocyte system. [0003] Interleukin 34 (IL-34) is a class of cytokines and proteins secreted by a variety of cells, including macrophages, endothelial cells, neuron...

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Problems solved by technology

In addition, IL-34 participates in the induction of concentration-dependent chemotaxis of macrophages by increasing the phosphorylation levels of focal adhesion kinase (FAK) and extracellular signal-related kinase 1 and 2 (ERK 1 / 2), but in vivo There is a lack of direct evidence that IL-34 induces macrophage aggregation, and most of the existing technologies are verified in in vitro cell line experiments, and there is still a lack of evidence in vivo experiments, and there is no real-time observation of macrophage aggregation in the liver in an in vivo environment direct evidence of

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