Application of angelica sinensis extract in preparation of anti-stress agent used for freshwater fish and preparation method of angelica sinensis extract
An angelica extract, anti-stress technology, applied in the field of freshwater fish farming, can solve problems such as fish stress response, inability to practically solve problems, and increase in morbidity and mortality of farmed fish.
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[0050] 1, a preparation method of angelica extract, comprising the steps of:
[0051] 1) Obtain 1000 g of root of Angelica sinensis, dry it in a blast drying oven at 50° C. to constant weight, crush it through a 18-mesh sieve, and obtain Angelica sinensis powder.
[0052] 2) Take 400g of angelica powder, mix according to the ratio of angelica powder and cyclohexane 1:6g / ml, rotate and stir at 600 rpm for 6 hours; filter the mixed solution to obtain clear filtrate and residue (repeat extraction 3 times); The filtrate was distilled under reduced pressure to constant weight to remove cyclohexane to obtain Angelica cyclohexane extract.
[0053] 3) According to the ratio of angelica cyclohexane extraction residue and diethyl ether 1: 6g / ml mixed, 600 rev / min rotating and stirring for 6 hours; the mixed solution was filtered to obtain a clear filtrate and residue (repeated extraction 3 times); the filtrate was reduced Pressure distillation to constant weight to remove ether, to obt...
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