Method for promoting proliferation of anaerobic ammonia oxidizing bacteria and application of method

Abstract

The invention provides a method for promoting the proliferation of anaerobic ammonia oxidizing bacteria, which utilizes gibberellin to promote the proliferation of the anaerobic ammonia oxidizing bacteria. The invention further provides an enrichment method of the anaerobic ammonia oxidizing bacteria; the method comprises the following steps: preparing an enrichment culture medium of the anaerobicammonia oxidizing bacteria; collecting sludge containing the anaerobic ammonia oxidizing bacteria and removing nitrogen background value; preparing an activated sludge solution containing the anaerobic ammonia oxidizing bacteria and inoculating the enrichment culture medium with the solution; adding the gibberellin and adding matrix ammonia nitrogen and nitrite nitrogen; regulating the pH (Potential of Hydrogen) value of a system to 8.0 to 8.3, so as to obtain a reaction system; carrying out anaerobic ammonia oxidizing reaction to obtain an anaerobic ammonia oxidizing system enriched with theanaerobic ammonia oxidizing bacteria. According to the method provided by the invention, the added gibberellin belongs to an organic matter and is not metabolized and utilized by the anaerobic ammonia oxidizing bacteria; a relatively excellent gibberellin adding concentration is preferred through adding the gibberellin with different gradient concentrations, and the anaerobic ammonia oxidizing bacteria with relatively high activity and relatively high abundance are obtained. Compared with a control test, when the concentration of the gibberellin is 1.0 mg / L, the removal rate of TN (Total Nitrogen) is improved by 34.1 percent.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering microorganisms, and in particular relates to a method for promoting the proliferation of anammox bacteria and an application thereof. Background technique [0002] With the improvement of people's living standards, a large amount of nitrogen is discharged into water bodies, which will lead to eutrophication of water bodies. Therefore, the removal of nitrogen in the process of sewage treatment has been highly valued by researchers. Traditional sewage biological denitrification technology not only needs to blow a large amount of air into the aerobic tank to maintain the supply of oxygen, but also needs to maintain the supply of carbon source in the anoxic tank, which consumes a lot of energy and resources. In addition, the traditional sewage biological denitrification technology also has the disadvantages of long process flow and large sludge production. Therefore, the development...

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Problems solved by technology

[0004] The growth rate of anammox bacteria is very slow, the generation cycle is about 11 days, the growth conditions are harsh, and they are easily affected by external environmental conditions (pH, DO, substrate concentration, temperature, light, etc.). The source is very lacking, which restricts its engineering application

[0005] There are many difficulties in the enrichment of anammox bacteria, and the cell yield of ANAMMOX bacteria is about 0.066mol C / molNH 4 + , the requirements for external environmental conditions are very strict, and people have not been able to obtain ANAMMOX strains through pure culture

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