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A method for improving the extraction yield of paeonifloride glycoside, the active ingredient of Radix Paeoniae Rubra

A technology for extracting active ingredients and ester glycosides, which is applied in the field of improving the extraction rate of paeonifloride glycosides, the active ingredient of Radix Paeoniae Rubra, can solve the problem of low extraction rate of active substances, and achieve the effect of improving the extraction rate

Active Publication Date: 2020-08-18
西安天行健天然生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of extracting paeoniflorin and paeoniflorin is the alcohol extraction method of paeoniflorin, and the extraction rate of effective substances in Radix Paeoniae Rubra is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for improving the extraction yield of paeonifloride, the active ingredient of Radix Paeoniae Rubra, comprising the following steps:

[0029] Q1: After washing the traditional Chinese medicinal materials of Radix Paeoniae Rubra, cut them into 3mm slices, place them at -25°C, freeze-dry them for 2 hours, and then perform ultrasonic wall-breaking treatment at a low temperature of -10°C to obtain the broken-walled Radix Paeoniae Rubra to be extracted. Chinese herbal medicine powder; the ultrasonic power of ultrasonic wall breaking is 800w, and the time is 40min.

[0030] Q2: Carry out enzymatic hydrolysis reaction treatment on the Chinese herbal medicine powder of Radix Paeoniae Rubra; specifically include the following steps:

[0031] Q21: For each kilogram of Radix Paeoniae Rubra powder, weigh 0.05g of pectinase, 0.05g of cellulase and 0.05g of amylase; then weigh the enzyme activator polyether and mix it with Radix Paeoniae Rubra root powder evenly, Obtain an e...

Embodiment 2

[0041] A method for improving the extraction yield of paeonifloride, the active ingredient of Radix Paeoniae Rubra, comprising the following steps:

[0042] Q1: After cleaning the traditional Chinese medicine of Radix Paeoniae Rubra, cut it into 4mm slices, place it at -50°C, freeze-dry it for 4 hours, and then perform ultrasonic wall-breaking treatment at a low temperature of -5°C to obtain the broken-walled Radix Paeoniae Rubra to be extracted Chinese herbal medicine powder; the ultrasonic power of ultrasonic wall breaking is 900w, and the time is 50min.

[0043] Q2: Carry out enzymatic hydrolysis reaction treatment on the Chinese herbal medicine powder of Radix Paeoniae Rubra; specifically include the following steps:

[0044] Q21: For every kilogram of Radix Paeoniae Rubra powder, weigh 0.02g of pectinase, 0.02g of cellulase and 0.02g of amylase; then weigh the enzyme activator polyether and mix it with the Radix Paeoniae Rubra root powder evenly, Obtain an enzyme drug mi...

Embodiment 3

[0054] A method for improving the extraction yield of paeonifloride, the active ingredient of Radix Paeoniae Rubra, comprising the following steps:

[0055] Q1: After cleaning the traditional Chinese medicine of Radix Paeoniae Rubra, cut it into 5mm slices, place it at -10°C, freeze-dry it for 5 hours, and then perform ultrasonic wall-breaking treatment at a low temperature of -15°C to obtain the broken-walled Radix Paeoniae Rubra to be extracted Chinese herbal medicine powder; the ultrasonic power of ultrasonic wall breaking is 1000w, and the time is 45min.

[0056] Q2: Carry out enzymatic hydrolysis reaction treatment on the Chinese herbal medicine powder of Radix Paeoniae Rubra; specifically include the following steps:

[0057] Q21: For each kilogram of Radix Paeoniae Rubra powder, weigh 0.03g of pectinase, 0.03g of cellulase and 0.03g of amylase; then weigh the enzyme activator polyether and mix it with the Radix Paeoniae Rubra root powder evenly, Obtain the enzyme drug ...

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PUM

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Abstract

The invention discloses a method for increasing the extraction yield of an effective component albiflorin of radix paeoniae rubra. The method comprises the following steps that Q1, the radix paeoniaerubra is cut into slices, and the slices are frozen, dried and subjected to ultrasonic wall-breaking treatment for obtaining radix paeoniae rubra Chinese herbal medicine powder; S2, pectinase, cellulase, amylase and an enzyme activator polyether are taken and mixed with the radix paeoniae rubra Chinese herbal medicine powder, and a mixture is placed in a reaction tank for enzymolysis to obtain a radix paeoniae rubra enzymolysis solution; Q3, a yeast fermentation solution, a cunninghamella blakesleeana fermentation solution and a radix paeoniae rubra enzymolysis solution are placed in an enzymolysis tank for a reaction according to the volume ratio of 1:1:50 so as to obtain a radix paeoniae rubra fermentation solution; Q4, purified water is added to the radix paeoniae rubra fermentation solution, stirring and extraction are conducted, and an extraction solution and filtering residues are separated; S5, the extraction solution is adsorbed by macroporous resin, washed and eluted, and a radix paeoniae rubra eluant is collected; the pH value of the eluant is adjusted, and concentration and recycling are conducted; freezing and drying are conducted for obtaining an albiflorin extract. According to the method, the extraction yield of the albiflorin in the radix paeoniae rubra can be effectively increased.

Description

technical field [0001] The invention belongs to the technical field of extraction of traditional Chinese medicines, and relates to a method for improving the extraction yield of paeonifloride glycoside, an effective component of Radix Paeoniae Rubra. Background technique [0002] In recent years, the production and extraction methods of Chinese herbal medicines have achieved a certain degree of development and mechanization, and there are many research papers on the extraction methods of Chinese herbal medicines. However, traditional Chinese herbal medicines are considered to have many impurities, unstable and low active ingredient content, and cannot be compared with modern medicine. In line with the standards, the development of modern Chinese medicine has been restricted. [0003] Paeoniflorin and paeonifloride glycosides are active ingredients extracted from the traditional Chinese medicine Radix Paeoniae Rubra, which can inhibit platelet aggregation, anticoagulant and a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14
Inventor 钱卫东张家宁沈兰芳刘淼孙照欢杨敏
Owner 西安天行健天然生物制品有限公司