Application of attenuated strain of Zika virus in the treatment of glioma
A Zika virus and glioma technology, applied in the field of tumor treatment, can solve the problems of short-term fever and the lack of further elucidation of the application of Zika virus
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Embodiment 1
[0030] This example is used to illustrate the neurovirulence characteristics of the Zika virus attenuated strain used in the present invention.
[0031] Take Zika virus attenuated strain (10650-10659 nucleotide deletion, see Chao Shan et al., Alive-attenuated Zika virus vaccine candidate induces sterilizing immunity inmouse models, Nature Medicine, 2017) and Japanese encephalitis virus ( Vaccine strains, purchased from Chengdu Institute of Biological Products Co., Ltd.) are tested as follows:
[0032] The above two viruses were divided into 1×10 4 The dose of PFU was intracranially inoculated into 3-week-old female BALB / c nude mice (purchased from Beijing Weitong Lihua Company), 8 mice were inoculated with each virus, and the morbidity and death of the mice were observed within 25 days, and the statistics were counted after 25 days of inoculation. The mortality rate (number of deaths / number of inoculations) of mice in this dose group was used to draw the survival curve of mic...
Embodiment 2
[0034] This example is used to illustrate the therapeutic effect of Zika virus attenuated strain on glioma in human glioma mouse model.
[0035] 1. Three-week-old female BALB / c nude mice were divided into two groups, and each mouse in the control group was intracranially injected with 50,000 glioma stem cells (GSCs, isolated from human malignant glia) that could stably express firefly luciferase tumor samples); in the experimental group, 50,000 cells of the same type were intracranially injected into each mouse, and 10,000 PFU of Zika virus attenuated strain (ZIKV-LAV) was given at the same time.
[0036]2. In vivo imaging experiments were performed on the 14th, 21st and 28th days after the intracranial injection, and the growth status of the brain tumor was evaluated by the fluorescence value. see results figure 2 . from figure 2 It can be seen that the tumor formation rates of mice in the control group were 3 / 9, 8 / 9, and 8 / 9 on days 14, 21, and 28 after intracranial inj...
Embodiment 3
[0039] This example is used to illustrate the therapeutic effect of Zika virus attenuated strain on glioma in human glioma mouse model.
[0040] Three-week-old female BALB / c nude mice were divided into two groups. Each mouse in the control group was injected intracranially with 50,000 glioma stem cells; each mouse in the experimental group was injected intracranially with 50,000 cells of the same type, and at the same time, 10,000 PFU Attenuated strain of Zika virus (ZIKV-LAV). On the 23rd day after the intracranial injection, all the mice were sacrificed and their brains were taken out. The brain tissue was made into frozen sections and then stained with H&E to observe the tumor size. see results Figure 4 . from Figure 4 It can be seen that the tumors in the brains of mice in the experimental group were much smaller than those in the control group.
[0041] It can be seen from the above results that the present invention can effectively treat glioma and prolong the surv...
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