Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Use of Ephrin-B1 in preparation of drug for treating inflammatory bowel disease

A technology for inflammatory bowel disease and its use, which is applied in the field of preparation of drugs for treating inflammatory bowel disease, and can solve problems such as increasing the risk of colorectal cancer, lack of treatment methods for inflammatory bowel disease, and protracted disease

Active Publication Date: 2019-08-06
武汉康理通科技有限公司
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no specific and effective treatment for inflammatory bowel disease, and the condition is often protracted, and chronic inflammatory bowel disease greatly increases the risk of colorectal cancer in patients1

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of Ephrin-B1 in preparation of drug for treating inflammatory bowel disease
  • Use of Ephrin-B1 in preparation of drug for treating inflammatory bowel disease
  • Use of Ephrin-B1 in preparation of drug for treating inflammatory bowel disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Experimental mice

[0026] C57BL / 6 mice were purchased from the Hubei Drug Safety Evaluation Center and raised in the SPF level animal laboratory of the Hubei Provincial Drug Safety Evaluation Center. 8-week-old male mice were selected for animal experiments. The mice were randomly divided into two groups, 10 in each group, one group was injected with control protein Fc, and the other group was injected with recombinant protein Ephrin-B1-Fc.

[0027] (2) Construction of pINFUSE-hIgG2-Fc2-Ephrin-B1-Fc vector

[0028] The full cDNA sequence of mouse Ephrin-B1 (shown in SEQ ID NO.4) was synthesized by the company and used as a template to construct the mouse Ephrin-B1 extracellular region expression vector (extracellular region The amino acid is the 30th lysine to the 229th serine of the mouse Ephrin-B1 protein). The expression vector is pINFUSE-hIgG2-Fc2, purchased from Invivogen.

[0029] The upstream primer for constructing the vector is: 5'-CATGCCATGGGCCACCCTGG...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses use of Ephrin-B1 in preparation of a drug for treating inflammatory bowel disease, which belongs to the field of a biological medicine. In the present invention, an Ephrin-B1 extracellular region coding sequence is constructed on an expression vector pINFUSE-hIgG2-Fc2, and a recombinant protein Ephrin-B1-Fc is expressed by mammalian cells, and the DSS-induced Mouse with colitis is subjected to intraperitoneal injection of Ephrin-B1-Fc, the Ephrin-B1-Fc can significantly alleviate the DSS-induced mouse enteritis, the weight loss of mice is reduced, the mortality of miceis reduced, the Ephrin-B1 can be used in the preparation of the drug for treating inflammatory bowel disease, and the Ephrin-B1 is human Ephrin-B1 (SEQ ID NO. 1) or mouse Ephrin-B1 (SEQ ID NO.3). Theapplication provides a novel direction for treating inflammatory bowel disease.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the use of Ephrin-B1 in the preparation of medicines for treating inflammatory bowel disease. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a chronic autoimmune disease involving the whole colon, mainly including ulcerative colitis (Ulcerative colitis, UC) and Crohn's disease (Crohn's disease, CD). In recent years, the incidence of inflammatory bowel disease in my country has been increasing year by year, and it has become an important cause of common diseases of the digestive system and chronic diarrhea, and most of the patients are young and middle-aged, so it has been paid more and more attention. There is currently no specific and effective treatment for inflammatory bowel disease, and the condition is often protracted, and chronic inflammatory bowel disease greatly increases the risk of colorectal cancer in patients 1 . Therefore, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/17A61P1/04C12N15/85C12N15/62C07K19/00
CPCA61K38/177A61K47/42C12N15/85C12N15/62C07K14/705C07K2319/30C12N2800/107
Inventor 王晨辉张华芝郭晓利
Owner 武汉康理通科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com