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Use of Ephrin-B1 in preparation of drug for treating inflammatory bowel disease

A technology for inflammatory bowel disease and its use, which is applied in the field of preparation of drugs for treating inflammatory bowel disease, and can solve problems such as increasing the risk of colorectal cancer, lack of treatment methods for inflammatory bowel disease, and protracted disease

Active Publication Date: 2019-08-06
武汉康理通科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no specific and effective treatment for inflammatory bowel disease, and the condition is often protracted, and chronic inflammatory bowel disease greatly increases the risk of colorectal cancer in patients1

Method used

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  • Use of Ephrin-B1 in preparation of drug for treating inflammatory bowel disease
  • Use of Ephrin-B1 in preparation of drug for treating inflammatory bowel disease
  • Use of Ephrin-B1 in preparation of drug for treating inflammatory bowel disease

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Experimental program
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Effect test

Embodiment 1

[0025] (1) Experimental mice

[0026] C57BL / 6 mice were purchased from the Hubei Drug Safety Evaluation Center and raised in the SPF level animal laboratory of the Hubei Provincial Drug Safety Evaluation Center. 8-week-old male mice were selected for animal experiments. The mice were randomly divided into two groups, 10 in each group, one group was injected with control protein Fc, and the other group was injected with recombinant protein Ephrin-B1-Fc.

[0027] (2) Construction of pINFUSE-hIgG2-Fc2-Ephrin-B1-Fc vector

[0028] The full cDNA sequence of mouse Ephrin-B1 (shown in SEQ ID NO.4) was synthesized by the company and used as a template to construct the mouse Ephrin-B1 extracellular region expression vector (extracellular region The amino acid is the 30th lysine to the 229th serine of the mouse Ephrin-B1 protein). The expression vector is pINFUSE-hIgG2-Fc2, purchased from Invivogen.

[0029] The upstream primer for constructing the vector is: 5'-CATGCCATGGGCCACCCTGG...

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Abstract

The invention discloses use of Ephrin-B1 in preparation of a drug for treating inflammatory bowel disease, which belongs to the field of a biological medicine. In the present invention, an Ephrin-B1 extracellular region coding sequence is constructed on an expression vector pINFUSE-hIgG2-Fc2, and a recombinant protein Ephrin-B1-Fc is expressed by mammalian cells, and the DSS-induced Mouse with colitis is subjected to intraperitoneal injection of Ephrin-B1-Fc, the Ephrin-B1-Fc can significantly alleviate the DSS-induced mouse enteritis, the weight loss of mice is reduced, the mortality of miceis reduced, the Ephrin-B1 can be used in the preparation of the drug for treating inflammatory bowel disease, and the Ephrin-B1 is human Ephrin-B1 (SEQ ID NO. 1) or mouse Ephrin-B1 (SEQ ID NO.3). Theapplication provides a novel direction for treating inflammatory bowel disease.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the use of Ephrin-B1 in the preparation of medicines for treating inflammatory bowel disease. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a chronic autoimmune disease involving the whole colon, mainly including ulcerative colitis (Ulcerative colitis, UC) and Crohn's disease (Crohn's disease, CD). In recent years, the incidence of inflammatory bowel disease in my country has been increasing year by year, and it has become an important cause of common diseases of the digestive system and chronic diarrhea, and most of the patients are young and middle-aged, so it has been paid more and more attention. There is currently no specific and effective treatment for inflammatory bowel disease, and the condition is often protracted, and chronic inflammatory bowel disease greatly increases the risk of colorectal cancer in patients 1 . Therefore, ...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P1/04C12N15/85C12N15/62C07K19/00
CPCA61K38/177A61K47/42C12N15/85C12N15/62C07K14/705C07K2319/30C12N2800/107
Inventor 王晨辉张华芝郭晓利
Owner 武汉康理通科技有限公司
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