Method for rapidly determining protein content of soybean grain
A technology for rapid determination of grain protein content, applied in the field of determination of soybean grain protein content, can solve the problems of high reagent consumption, long time required, and inapplicability, and achieve the effect of improving measurement efficiency and realizing automation
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[0030] A method for quickly determining the protein content of soybean grains, including the following steps:
[0031] a. Select soybean grains for pretreatment: use at least one of Rhizobium fredii sinensis liquid and biostimulant solution to add distilled water to dress soybean grains, and the concentration of rhizobium fredii sinensis liquid is 2.86×10 9 A. mL -1 , The concentration of the biostimulant solution is 40g. L -1 Or 80g﹒ L -1 ;
[0032] b. Collect the pretreated soybean grains, grind, dry, and pass through a 0.5mm sieve, and then weigh 0.1-0.5g of soybean grains;
[0033] c. Place the soybean grains weighed in step b in a 50mL opener bottle, first drip 1mL of distilled water for wetting, then add 5mL of concentrated sulfuric acid, and shake it to form a digestion liquid;
[0034] d. Place the Ketchup bottle with the digestion liquid in step c on the digestion furnace for heating, set the heating temperature to 250°C, and set the heating temperature to 330℃ when the dig...
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