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Application of vnn1 in plasma or serum as a marker for early warning of sepsis

A sepsis and serum technology, applied in clinical medicine and biological fields, can solve the problems of short retention time, great variation in results, low specificity, etc., and achieve the effect of improving early performance and accuracy and reducing the incidence rate.

Active Publication Date: 2022-05-17
中国人民解放军陆军特色医学中心
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AI Technical Summary

Problems solved by technology

C-reactive protein (CRP), procalcitonin (PCT) and other inflammatory indicators that have been used in clinical practice can only reflect the degree of systemic inflammatory response in patients at the time of detection, and the results vary greatly with different detection time windows. Inflammatory cytokines such as IL-6 and TNFα have a short retention time in the blood, and it is difficult to accurately grasp the appropriate timing of detection; while scoring systems based on body anatomy and pathophysiology, such as AIS, ISS, SOFA, APACH III etc., and cannot reflect the disease development and prognosis of trauma patients
Therefore, these indicators can only be used as auxiliary indicators for the diagnosis and monitoring of trauma infection. So far, no specific indicators for early warning of trauma sepsis have been found. Therefore, a new high-sensitivity and specific early warning of trauma sepsis has been discovered. Diagnostic biomarkers are critical for early detection, early diagnosis, and early treatment

Method used

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  • Application of vnn1 in plasma or serum as a marker for early warning of sepsis
  • Application of vnn1 in plasma or serum as a marker for early warning of sepsis
  • Application of vnn1 in plasma or serum as a marker for early warning of sepsis

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Embodiment 1

[0021] Detection of VNN1 in the blood of patients with severe trauma and its expression changes in patients with posttraumatic sepsis

[0022] Plasma samples were collected from 16 healthy persons and 30 severe trauma patients on days 1, 3, 5, 7, and 14 after injury, and the expression of VNN1 was detected with an ELISA kit (purchased from Cloud-Clone Corp). The specific operation steps were strict. Refer to kit instructions. SPSS 17.0 software was used for statistical analysis, and GraphPad Prism 6.0 software was used for graphing.

[0023] The results showed that compared with healthy people (0.66±0.12ng / ml), the level of VNN1 in the plasma of severely traumatized patients was significantly increased (3.05±3.37ng / ml) at 1 day after injury, and the expression of VNN1 increased over time. The level gradually decreased, but still higher than that of healthy people ( figure 1 ) (P figure 2 ), and as the severity of sepsis increased, the expression of VNN1 increased (sepsis: 1....

Embodiment 2

[0025] ROC curve analysis to compare the early warning efficacy of plasma PCT, CRP and VNN1 on posttraumatic sepsis

[0026] The samples of 283 severe trauma patients were collected 1 day after injury, and the PCT and CRP detection values ​​of the corresponding patients in the Chinese Trauma Biobank were collected at the same time. The ROC curve was drawn by SPSS19.0 software, and the statistics of the area under the ROC curve were compared by MedCalc15.8 software. academic difference.

[0027] The results showed that the area under the ROC curve of plasma PCT was 0.576 (0.516-0.634), the sensitivity was 32.97%, the specificity was 98.96%, and the cut-off value was 6ng / ml; the area under the ROC curve of CRP was 0.623 (0.564-0.680) , the sensitivity was 37.36%, the specificity was 84.37%, the cutoff value was 79.8mg / l; the area under the ROC curve of VNN1 was 0.822 (0.773-0.865), the sensitivity was 70.33%, the specificity was 84.90%, the cutoff value The area under the curve...

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Abstract

The invention relates to the application of a blood vessel non-inflammatory factor (VNN1) in plasma or serum as a marker for early warning of sepsis. The present invention uses VNN1 as a biomarker, and the applicant has verified through experiments that the level of VNN1 in plasma of patients with severe trauma is significantly higher than that of normal people, and the level of plasma VNN1 in patients with sepsis after trauma is significantly higher than that in patients without sepsis . By detecting the level of plasma or serum VNN1, early prediction of the risk of sepsis in severe trauma patients, so as to give individualized intervention treatment, can reduce the incidence of post-traumatic sepsis, and has important clinical application value in improving the prognosis of severe trauma patients .

Description

technical field [0001] The invention relates to the fields of clinical medicine and biotechnology, in particular to the application of vascular non-inflammatory factor (VNN1) in plasma or serum as a marker for early warning of sepsis. Background technique [0002] Trauma is a global public health problem, with 4.8 million deaths each year, accounting for 10% of the global disease burden. With the advancement of pre-hospital first aid technology and the improvement of the treatment system, the direct mortality rate caused by injuries in the early stage of trauma has been significantly reduced, but late trauma complications such as sepsis still seriously threaten the survival of patients. [0003] Sepsis, an infection-induced systemic inflammatory response syndrome, is a common complication and one of the leading causes of death in severely traumatized patients. Further development can lead to septic shock and multiple organ dysfunction syndrome (Multiple Organ Dysfunction Sy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/573
CPCG01N33/573G01N2333/98G01N2800/7095G01N2800/50
Inventor 陆红祥张安强蒋建新顾玮曾灵杜娟文大林孙剑会
Owner 中国人民解放军陆军特色医学中心
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