Bifidobacterium pseudosmall chain ccfm1048, its composition, fermented food, use, bacterial agent and preparation method of bacterial agent

A bifidobacterium and fermented food technology, applied in the field of microorganisms, can solve the problems of irritating the gastrointestinal tract, liver function damage, affecting patients, etc., and achieve the effect of alleviating weight gain, alleviating metabolic syndrome, and reducing the lower area

Active Publication Date: 2021-12-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs have a good curative effect on each single risk factor of metabolic syndrome. However, long-term use of drugs has certain side effects. For example, long-term use of metformin can cause irritation to the gastrointestinal tract of some patients and cause discomfort and may affect the patient’s response to vitamin B12. The absorption of rosiglitazone can cause liver function damage and edema, etc.

Method used

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  • Bifidobacterium pseudosmall chain ccfm1048, its composition, fermented food, use, bacterial agent and preparation method of bacterial agent
  • Bifidobacterium pseudosmall chain ccfm1048, its composition, fermented food, use, bacterial agent and preparation method of bacterial agent
  • Bifidobacterium pseudosmall chain ccfm1048, its composition, fermented food, use, bacterial agent and preparation method of bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1: Pseudo-small chain Bifidobacterium CCFM1048 has good tolerance to simulated gastrointestinal fluid

[0085] Inoculate the cryopreserved Bifidobacterium CCFM1048 in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), culture anaerobically at 37°C for 48 hours, and then subculture in mMRS medium for 2 After ~3 times, take 1mL of the culture solution of Bifidobacterium pseudospermia CCFM1048, mix it with 9.0mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and anaerobically For culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, poured and cultured with mMRS agar medium to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0086] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, expressed in %. ...

Embodiment 2

[0091] Example 2: Pseudomonas Bifidobacterium CCFM1048 has no toxic side effects on C57BL / 6J mice

[0092] The pseudo small chain Bifidobacterium CCFM1048 bacteria were resuspended in 3% sucrose solution to make a concentration of 3.0×10 8 CFU / mL bacterial suspension. Six healthy male C57BL / 6J mice with a body weight of about 20 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0093] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 The CFU / mL pseudosmall chain Bifidobacterium CCFM1048 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0094] Table 3 Changes in body weight and death of mice

[0095]

[0096] Note: -: no death of mice

Embodiment 3

[0097] Example 3: Pseudomonas bifidobacterium CCFM1048 has a recovery effect on the intestinal flora imbalance caused by high-fat feed

[0098] 36 healthy male C57BL / 6J mice weighing 18-20 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-fat model control group (HFD), simvastatin control group (SC ), rosiglitazone control group (RH), Bifidobacterium pseudospermia CCFM1048 intervention group (CCFM1048), Bifidobacterium animalis BB12 control group (BB12), each group contained 6 mice, and the bacteria suspension The dose is 3.0 x 10 8 CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:

[0099] Table 4 Grouping of experimental animals

[0100]

[0101] The body weight of the mice was monitored regularly during the experiment.

[0102] At the end of the experiment, the fresh feces of the mice were collected and frozen at -80°C, the ...

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Abstract

The invention discloses Bifidobacterium pseudosmallis CCFM1048, its composition, fermented food, application, bacterial agent and preparation method thereof. The Bifidobacterium pseudoserinus CCFM1048 can tolerate human gastrointestinal environment and significantly improve high-fat diet The resulting increase in liver triglyceride content in mice with metabolic syndrome; alleviated weight gain in mice with metabolic syndrome; significantly improved fasting blood glucose and abnormal oral glucose tolerance in mice with metabolic syndrome, and reduced the area under the curve of glucose tolerance drugs. Improve serum insulin levels; significantly improve serum low-density lipoprotein cholesterol levels in mice with metabolic syndrome caused by high-fat diets; significantly improve the increase in intestinal Proteus species caused by high-fat diets, CCFM1048 is used to prepare and relieve metabolism Syndrome, reducing Parkinson's disease, intestinal infection, urinary tract, kidney infection and other diseases, the pharmaceutical composition and fermented food have a very wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to pseudosmall chain bifidobacterium CCFM1048, its composition, fermented food, application, bacterial agent and preparation method thereof. Background technique [0002] In recent years, with the continuous development of our country's social and economic level, the living standards of the public have continued to improve, the proportion of carbohydrates and fats in the diet structure has increased day by day, and the restrictions of many working conditions have made it easy for people to develop sedentary behavior Habit. These external environmental factors combined with their own genetic factors have led to the continuous increase in the incidence of metabolic syndrome in the Chinese population. According to reports, between 2002 and 2010, the proportion of people over the age of 20 in my country with metabolic syndrome rose from 5.4% to 21.3%, an increase of 4 time...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P3/00A61P3/06A61P3/10A61P25/16A61P1/00A61P13/02A61P13/12A23C9/123A23C13/16A23C19/06A23L11/50A23L19/20A23L33/00C12R1/01A23L11/60
CPCC12N1/20A61P3/00A61P3/06A61P3/10A61P25/16A61P1/00A61P13/02A61P13/12A23C9/1234A23C13/16A23C19/062A23C11/106A23L19/20A23L33/00A61K2035/115A23V2002/00A23L11/50C12R2001/01C12N1/205A23V2400/535A23V2200/30
Inventor 王刚陈卫刘煜飞赵建新张灏
Owner JIANGNAN UNIV
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