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ARF gene of rubber trees and coded protein and application thereof

A rubber tree, gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, to achieve the effect of promoting growth, improving economic benefits, and shortening the non-productive growth period

Active Publication Date: 2019-08-09
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, this gene has not been reported in rubber tree

Method used

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  • ARF gene of rubber trees and coded protein and application thereof
  • ARF gene of rubber trees and coded protein and application thereof
  • ARF gene of rubber trees and coded protein and application thereof

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Embodiment Construction

[0026] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention.

[0027] 1. Cloning and analysis of rubber tree HbARF2 gene

[0028] Utilize RT-PCR technology to clone and obtain the HbARF2 gene of the rubber tree transcription factor from the bark of the Brasilian rubber tree variety Reyan 8-79, and the specific implementation scheme is as follows:

[0029] The total RNA of rubber tree bark was extracted according to the operation instructions of Tiangen RNAprep Pure Polysaccharide and Polyphenol Plant Total RNA Extraction Kit. The first strand of cDNA was synthesized according to the operation steps of the Ferments kit. According to the transcriptome database of our laboratory, the Unigene annotated as ARF was obtained, and the complete gene sequence was preliminarily identified by searching and comparing with the NCBI database. Using the first strand ...

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Abstract

The invention provides an ARF gene of rubber trees. The ARF gene is a rubber tree growth inhibition factor HbARF2 gene, and the nucleotide sequence of the ARF gene is shown as SEQ ID NO:1. The invention also provides a protein coded by the HbARF2 gene and application of the protein. The HbARF2 gene is cloned from the rubber trees for the first time and is a transcriptive gene. It is verified by transgenic plants that the HbARF2 gene can delay the growth and development of stems, and it is indicated that the HbARF2 gene plays an important role in regulating and controlling the stems and the like of the rubber trees. Serving as a main source of natural rubber, the Brazilian rubber trees have a non-productive growth period which can last for 6-8 years; thus, by studying the molecular mechanism of HbARF2, knocking out genes and carrying out genetic improvement, the growth of the rubber trees is promoted, shortening of the non-productive growth period of the rubber trees is facilitated, theeconomic benefits of the rubber trees are increased, and meanwhile, a great basis is provided for other target breeding setting.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an ARF gene of a rubber tree, its encoded protein and its application. Background technique [0002] Auxin response factor (auxin response factor, ARF) is a kind of transcription factor that can activate or inhibit, and can specifically bind to AuxRE in the promoter of auxin initial response gene. Through the regulation of auxin synthesis, it affects the growth and development of plants at various stages. ARF activates or inhibits the expression of target genes depending on the amino acid sequence of the intermediate region between the functional domain and DNA interaction. ARF protein consists of three parts: DNA-binding domain (DNA-bindingdomain, DBD), middle region (middle region, MR) and C-terminal domain (Phox and Bem1domain, PB1). DBD is directly involved in the combination with AuxRE; MR is a non-conserved region, which activates or inhibits the function of targe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/10C12N15/82C12N15/11C07K14/415A01H5/04
CPCC07K14/415C12N15/8261
Inventor 李言田维敏
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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