Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker for identifying trachinotus ovatus and trachinotus blochii and application of molecular marker

A technology of molecular markers and oval pompano, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of marine species mixing, and achieve easy mastery, strong repeatability, The effect of avoiding economic loss

Inactive Publication Date: 2019-08-09
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
View PDF6 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above problems, the purpose of the present invention is to provide a molecular marker primer and application thereof for distinguishing oval pomfret and Bryce pomfret, which helps to solve problems such as marine species mixing, for pomfret breeding, pomfret resource protection, reasonable Use and provide technical support for biodiversity research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for identifying trachinotus ovatus and trachinotus blochii and application of molecular marker
  • Molecular marker for identifying trachinotus ovatus and trachinotus blochii and application of molecular marker
  • Molecular marker for identifying trachinotus ovatus and trachinotus blochii and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:

[0027] 1) Select 5 individuals of Pomfret Oval and Pompano Brycei species collected from the coastal waters of Sanya, Hainan to form a sample group, and extract the DNA of the two sample groups respectively;

[0028] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;

[0029] The composition of described PCR reaction system is:

[0030] Add in order to the 0.2ml centrifuge tube:

[0031]

[0032] The PCR amplification program was pre-denaturation at 94°C for 2 min, (denaturation at 94°C for 15 sec, annealing at 50°C for 15 sec, extension at 72°C for 15 sec) x 38 cycles, extension at 72°C for 3 min, and storage at 4°C for long-term storage.

[0033] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populations to identify thei...

Embodiment 2

[0035] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:

[0036] 1) Select 5 individuals of pomfret ovata and pomfret pomfret seedlings collected from the coastal waters of Sanya, Hainan, respectively, to form a sample group, and extract the DNA of the two sample groups respectively;

[0037] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;

[0038] The composition of described PCR reaction system is:

[0039] Add in order to the 0.2ml centrifuge tube:

[0040]

[0041] The PCR amplification program was pre-denaturation at 94°C for 4 min, (denaturation at 94°C for 35 sec, annealing at 53°C for 30 sec, extension at 72°C for 15 sec) x 30 cycles, extension at 72°C for 10 min, and storage at 4°C for long-term storage.

[0042] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populati...

Embodiment 3

[0044] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:

[0045] 1) Select 5 individuals of pomfret ovata and pomfret pomfret seedlings collected from the coastal waters of Sanya, Hainan, respectively, to form a sample group, and extract the DNA of the two sample groups respectively;

[0046] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;

[0047] The composition of described PCR reaction system is:

[0048] Add in order to the 0.2ml centrifuge tube:

[0049]

[0050] The PCR amplification program was pre-denaturation at 94°C for 5 min, (denaturation at 94°C for 35 sec, annealing at 50°C for 35 sec, extension at 72°C for 35 sec) x 38 cycles, extension at 72°C for 10 min, and storage at 4°C for long-term storage.

[0051] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention aims at providing a molecular marker for identifying trachinotus ovatus and trachinotus blochii and application of the molecular marker. The molecular marker contributes to solving the problems of marine species mixing and the like, and technical support is provided for pompano breeding, pompano resource protection and reasonable utilization and biodiversity studies. The molecular marker for identifying the trachinotus ovatus and the trachinotus blochii comprises a primer CSF and a primer CSR, the specific sequence of CSF is GTCATACGCTCCCGAAGAT, and the specific sequence of CSR is TAGGGCTAAGCATAGTGGG. The molecular marker is used for identifying the trachinotus ovatus and the trachinotus blochii. A kit containing the molecular marker primers and a PCR system reagent is applied to identifying the trachinotus ovatus and the trachinotus blochii. The molecular marker identifying the trachinotus ovatus and the trachinotus blochii is further used for identifying offspring seedsof the trachinotus ovatus and the trachinotusblochii. The result indicates that the two kinds of fishes differ in two basic groups, which is shown as a conversion and a transversion, the result is stable, and the repeatability is high.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a molecular marker primer for distinguishing pomfret seedlings from oval pomfret seedlings and pomfret carp seedlings and an application thereof. Background technique [0002] Both Trachinotus ovatus and Trachinotus blochii belong to the order Perciformes, family Carangidae, genus Trachinotus, and are widely distributed in the temperate regions of the Indian Ocean, the Pacific Ocean and the Atlantic Ocean. , Tropical waters. Both pomfret and pomfret are marine fish with high economic value. The cage culture of these two species of pomfret has been carried out since the 1990s along the coasts of Hainan, Guangdong and Fujian. Because the two kinds of pomfret are very similar in shape, they are called yellow pomfret, seawater white pomfret or golden pomfret in coastal areas of China. Although the two types of pomfret are very similar, the temperature adaptabi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124
Inventor 单斌斌孙典荣刘岩杨长平赵雨
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com