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Ultralow-temperature sample-changeable microscopic imaging system and working method thereof

A microscopic imaging and ultra-low temperature technology, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problems affecting the experimental throughput and success rate, the sample is easily damaged, and the operation is complicated, so as to improve the mechanical stability and imaging quality. , the effect of improving image quality and resolution

Pending Publication Date: 2019-08-09
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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Problems solved by technology

This process is complicated to operate and requires trained professionals, and the sample is easily damaged or contaminated by ice during the transfer process, which seriously affects the throughput and success rate of the experiment

Method used

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  • Ultralow-temperature sample-changeable microscopic imaging system and working method thereof
  • Ultralow-temperature sample-changeable microscopic imaging system and working method thereof
  • Ultralow-temperature sample-changeable microscopic imaging system and working method thereof

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Embodiment Construction

[0034] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and examples.

[0035] An ultra-low temperature replaceable sample microscopic imaging system, including a light source system, a microscopic imaging system, and a vacuum replaceable sample cold stage system; wherein the light source system is used to provide the light source required for exciting fluorescence or bright field illumination, etc., and the microscopic imaging system It is used to receive fluorescence signals or bright field signals for imaging, and the vacuum interchangeable cold stage system is used to provide samples with an ultra-low temperature vacuum environment and as a carrier for microscope objectives. The light source system is a multi-channel coupled light source; the microscopic objective lens of the microscopic imaging system is placed in a vac...

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Abstract

The invention discloses an ultralow-temperature sample-changeable microscopic imaging system and a working method thereof. The system comprises a light source system, a microscopic imaging system anda vacuum sample-changeable cold platform system; wherein the light source system is used for providing light sources required by fluorescence excitation or bright field illumination and the like, themicroscopic imaging system is used for receiving fluorescence signals or bright field signals and the like for imaging, the vacuum sample-changeable cold stage system is used for providing an ultralow-temperature vacuum environment for a sample and serving as a carrier of a microscope objective, and the microscope objective of the microscopic imaging system is placed in the vacuum sample-changeable cold stage system. The ultralow-temperature sample-changeable microscopic imaging system and the working method thereof can realize the fluorescence microscopic imaging in the ultralow-temperature vacuum environment, can improve the resolution of the microscopic imaging, and has the advantages of easy operation, stable system, high imaging quality and the like.

Description

technical field [0001] The invention relates to the technical field of cryo-photoelectric correlation imaging, in particular to an ultra-low temperature replaceable sample microscopic imaging system and a working method thereof. Background technique [0002] Cryofluorescence imaging is an imaging technology that has just emerged in the world. It freezes fluorescently labeled biological samples at the temperature of liquid nitrogen or liquid helium for optical imaging. Compared with room temperature imaging, fluorescence imaging under freezing conditions has the following advantages: the sample is in a fixed state under freezing conditions, and the thermal motion of molecules at the nanometer level is almost stopped, which is a prerequisite for analyzing the nanostructure of samples; the preparation method of frozen samples ( Such as high-pressure cryosection, quick freezing) can freeze the biological structure in an almost original state, avoiding the interference introduced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6458G01N2021/6439
Inventor 李尉兴纪伟徐涛付彦辉
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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