Method for breeding blast-resistant multi-line hybrid varieties using rice temperature-sensitive permanent genetic male sterile lines

Abstract

The invention discloses a method for breeding multi-line hybrid varieties resistant to blast disease by using rice temperature-sensitive permanent nuclear sterile lines. The method comprises the following steps: screening rice blast-resistant germplasm materials—transferring—test crossing—test mating—final selection to obtain parental materials of rice temperature-sensitive permanent genetic male sterile lines carrying different disease resistance genes, Obtain 2 (or more) rice temperature-sensitive permanent genetic male sterile lines heterozygous for disease-resistant genes through breeding and production, and then test and match with disease-resistant multi-line restorer lines to breed rice blast-resistant multi-line hybrid varieties. The invention utilizes hybridization technology to introduce rice blast resistance genes from different sources into thermosensitive genic male sterile lines and anti-thermosensitive genic male sterile lines respectively, and then through bridge parents to breed disease-resistant close isogenic lines of bridge parents. Except for the sterility gene and different disease resistance genes, other traits are basically the same. Through the outcrossing of disease-resistant near-isogenic lines, the temperature-sensitive permanent nuclear male sterile line of disease-resistant rice is produced, which is a new two-line hybrid rice variety with multiple resistance. The foundation was laid, which not only solved the problem of the fertility of the temperature-sensitive GMS line and the anti-therm-sensitive GMS line, but also solved the problem of rice blast damage in large-scale production.

Description

technical field [0001] The invention belongs to the innovation field of new materials utilizing rice heterosis, and in particular relates to a method for selecting rice blast-resistant multi-line hybrid varieties by using rice temperature-sensitive permanent nuclear sterile lines. Background technique [0002] Rice blast is one of the most serious diseases of rice. It is widely distributed and causes serious damage. In popular years, rice production can be reduced by 10% to 20%. It is estimated that the annual loss of rice caused by rice blast is enough to feed more than 60 million people. The methods of controlling rice blast are mainly chemical control and popularization of new disease-resistant varieties. Although chemical control plays a very important role in stabilizing rice production, the problems it brings are becoming more and more prominent: on the one hand, it increases the labor cost of farmers, on the other hand, it will cause serious environmental pollution a...

AI Technical Summary

Problems solved by technology

Although chemical control plays a very important role in stabilizing rice production, the problems it brings are becoming more and more prominent: on the one hand, it increases the labor cost of farmers, on the other hand, it will cause serious environmental pollution and endanger the biodiversity of nature; At the same time, a large amount of pesticide residues pose a potential threat to human health