A kind of crossbreeding method of spotted catfish and catfish
A technology of spotted catfish and catfish, applied in the fields of application, climate change adaptation, fish farming, etc.
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Embodiment 1
[0041] The spotted catfish was used as the female parent, and the selection criteria were: the spotted catfish had a swollen and soft abdomen, and the genital opening was reddish. The weights of the three-tailed catfish used for dry artificial insemination were 345g, 360g and 375g, respectively.
[0042] The male catfish is used as the male parent, and the selection criteria are as follows: the male fish has a strong and plump appearance, is full of luster, has a reddish reproductive process, and has reached the age of sexual maturity. The body weight of a catfish used for dry artificial insemination is 1400g.
[0043] Get a tail body weight and be the pituitary gland of 1080g Rusta wild carp, make 1.08ml pituitary gland solution with 0.9% sodium chloride solution.
[0044] 0.1035ml, 0.108ml and 0.1125ml of Rusta rohu pituitary solution were respectively injected for the first time to the spotted catfish whose body weight was 345g, 360g and 375g respectively, and 0.2415ml, 0.2...
Embodiment 2
[0050] The spotted catfish was used as the female parent, and the selection criteria were as follows: the spotted catfish had a swollen and soft abdomen, reddish genital pores, and the body weights of the three-tailed catfish used for dry artificial insemination were 360g, 355g and 365g, respectively.
[0051] The male catfish is used as the male parent, and the selection criteria are as follows: the male fish has a strong and plump appearance, is full of luster, has a reddish reproductive process, and has reached the age of sexual maturity. A catfish catfish used for dry artificial insemination weighs 1500g.
[0052] Get a tail body weight and be the pituitary gland of 1080g Rusta wild carp, make 1.08ml pituitary gland solution with 0.9% sodium chloride solution.
[0053] For the first time, 0.108ml, 0.1065ml and 0.1095ml of the pituitary solution of rohu lusta were respectively injected to the spotted catfish whose body weight was 360g, 355g and 365g, respectively, and 0.252...
Embodiment 3
[0059] The spotted catfish was used as the female parent, and the selection criteria were: the spotted catfish's abdomen was enlarged, soft, and the genital pores were reddish. The weights of the three-tailed catfish used for dry artificial insemination were 370g, 380g and 360g, respectively.
[0060] The male catfish is used as the male parent, and the selection criteria are as follows: the male fish has a strong and plump appearance, is full of luster, has a reddish reproductive process, and has reached the age of sexual maturity. A catfish catfish used for dry artificial insemination weighs 1600g.
[0061] Get a tail body weight and be the pituitary gland of 1110g Rusta wild carp, make 1.11ml pituitary gland solution with 0.9% sodium chloride solution.
[0062] For the first time, 0.111ml, 0.114ml and 0.108ml of the pituitary solution of rohu lusta were injected respectively to the spotted catfish with the body weight of 370g, 380g and 360g, respectively, and 0.259ml, 0.266...
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