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A kind of heat-resistant Lactobacillus plantarum and its application in agricultural production

A technology of Lactobacillus plantarum and peanuts, applied in plant growth regulators, applications, agriculture, etc., can solve the problems of limiting the rapid decomposition of organic matter, reducing the number and activity of lactic acid bacteria, and poor heat resistance of lactic acid bacteria, so as to achieve agricultural health and shorten Effect of composting time and strong heat resistance

Active Publication Date: 2022-07-12
QINGDAO LILIHUI BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the heat resistance of lactic acid bacteria is poor, and the optimum growth temperature of different bacterial genera is roughly 35-45°C
Therefore, under the influence of high temperature factors, the population and activity of lactic acid bacteria will generally decrease during the high temperature period of composting, thus limiting the rapid decomposition of organic matter

Method used

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  • A kind of heat-resistant Lactobacillus plantarum and its application in agricultural production
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  • A kind of heat-resistant Lactobacillus plantarum and its application in agricultural production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening and identification of high temperature resistant lactic acid bacteria

[0034] 1.1 Screening of high temperature resistant lactic acid bacteria

[0035] Sample: Sludge at the bottom of the biogas tank of the farm in Pingdu Lilan Town, Qingdao City, Shandong Province.

[0036] The sludge samples were enriched and cultured at 70°C, and 55 high-temperature-resistant pure strains were isolated by gradient dilution method. The isolated strains were inoculated to the MRS plate (10g of peptone, 5g of yeast extract, 10g of beef extract, 20g of glucose, 2g of dipotassium hydrogen phosphate, 2g of ammonium citrate, 5g of sodium acetate, 0.58g of magnesium sulfate) added with 0.5% calcium carbonate respectively. , manganese sulfate 0.25g, Tween 80mL, agar 15g, distilled water 1.0L, pH 6.5±0.2), cultured at 37°C for 48h, isolated and cultured strains with transparent circles, and obtained 3 strains of lactic acid bacteria, named LLH-1 , LLH-2, LLH-3;

[0037]...

Embodiment 2

[0049] Example 2 Determination of protease activity of Lactobacillus plantarum LLH-2

[0050] Lactobacillus plantarum LLH-2 was inserted into MRS liquid medium (10g peptone, 5g yeast extract, 10g beef extract, 20g glucose, 2g dipotassium hydrogen phosphate, 2g ammonium citrate, 5g sodium acetate, 0.58g magnesium sulfate, manganese sulfate 0.25g, 801mL of Tween, 1.0L of distilled water, pH6.5±0.2), cultured at 37°C for 24h, transferred and activated for two generations continuously, and inserted into 200mL of MRS liquid culture with 1% ( / v / v) inoculum In the medium, cultured at 37°C for 48h; centrifuged at 8000r / min for 10min at 4°C, collected the supernatant, and detected the protease activity in the supernatant by the following method.

[0051] The results show that the protease activity in the fermentation supernatant of Lactobacillus plantarum LLH-2 screened by the present invention is as high as 97 U / mL, and an unexpected technical effect is achieved.

[0052] (1) Definit...

Embodiment 3

[0056] Example 3 Application of Lactobacillus plantarum LLH-2 in composting

[0057] 1. The compost materials are chicken manure and sawdust, which are respectively taken from the chicken farm and wood processing factory in Pingdu, Qingdao.

[0058] 2. Experimental group settings:

[0059] (1) Blank control group: inoculated with sterile water;

[0060] (2) CGMCC1.572 treatment group: inoculated with Lactobacillus plantarum CGMCC1.572;

[0061] (3) LLH-2 treatment group: inoculated with Lactobacillus plantarum LLH-2.

[0062] According to the properties of chicken manure and sawdust, adjust the moisture to about 60%, and adjust the C / N ratio to 25-30. After fully mixing, put it into a foam box with a volume of 4L.

[0063] Before compost inoculation, the Lactobacillus plantarum fermentation broth ((10 8 -10 9 cfu / ml)) with 5‰ of the wet weight of the material and cornmeal at a ratio of 1:1 by volume and mass to be fully mixed, and inoculated into the compost material when...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and specifically provides a thermotolerant Lactobacillus plantarum and its application in agricultural production. The Lactobacillus plantarum was screened from the sludge at the bottom of the biogas tank, and the deposit number is CCTCC NO: M2019401. The strain has strong heat resistance, strong acid-producing and protease-producing ability, and obvious bacteriostatic effect, and can be widely used in agricultural production.

Description

technical field [0001] The invention relates to the technical field of screening and application of functional microorganisms, in particular to a novel heat-resistant Lactobacillus plantarum and its application in agricultural production. Background technique [0002] Modern composting technology was successfully developed and researched by developed countries in the early 20th century, and has been widely used in Britain, the United States, France, Germany, Japan and other countries. About 49% of the sludge in the United States is used as fertilizer on farmland or forest land each year. France has also applied the BIOTERRA mechanical composting process with good results. The traditional composting method in my country generally adopts the method of increasing nutrition and improving environmental conditions, and uses the indigenous microorganisms in the composting raw materials to degrade organic pollutants. The decomposition rate of microorganisms varies greatly, so tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C05F17/50C05G3/60A01N63/20A01P3/00C12R1/25
CPCC05F3/00C05F17/00C05G3/00A01N63/00C12R2001/25C12N1/205C05F11/00C05F11/08C05F5/00C05F5/002C05F5/006C05F7/00C05F9/00Y02W30/40
Inventor 孙春龙代庆海王丽宁王凌云林俊荣李德红周文慧贾爱宾
Owner QINGDAO LILIHUI BIOTECH