A method for the detection of cholesterol based on rgo-cs-fc/pt NPs nanocomposite
A technology of nano-composite materials and composite nano-materials, which is applied in the direction of analyzing materials, electrochemical variables of materials, and material analysis through electromagnetic means. Achieve the effects of short detection time, easy miniaturization, and simple operation
Active Publication Date: 2021-09-17
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology
[0003] The technical problem to be solved by the present invention is to provide an enzyme electrode using graphene-chitosan-ferrocene composite material (RGO-CS-Fc) combined with platinum nanoparticles (Pt NPs), and then use electrochemical methods to detect cholesterol In order to solve the problems of current biosensors such as susceptibility to environmental influence, poor conductivity and high technical requirements
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[0037] The present invention will be described in detail below in combination with specific embodiments. This embodiment is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any form. Any simple modifications made to the above embodiments according to the technical essence of the present invention, equivalent changes and modifications, all belong to this invention. within the scope of the technical solution of the invention.
[0038] figure 1 Schematic diagram of the method for constructing an enzyme electrode based on RGO-CS-Fc / Pt NPs to detect cholesterol. Au NPs were deposited on the SPCE surface by potentiostatic deposition to increase the effective area of the electrode. Then, the RGO-CS-Fc nanocomposite was immobilized on the SPCE / Au NPs electrode surface through the interaction of π-π bonds and electrostatic adsorption. Platinum nanoparticles were deposited on the surface of SPCE / Au NPs / RGO-CS-Fc electrode b...
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Abstract
The invention discloses a method for detecting cholesterol based on RGO-CS-Fc / Pt NPs nanocomposite material. First, an electrode is screen-printed on the basis of activation treatment, and a layer of gold nanoparticles is formed on the surface by an electrodeposition method, and the RGO-CS-Fc / Pt NPs nanocomposite Fc is modified to the electrode surface, and then platinum ions are electroreduced on the electrode surface by a potentiostatic deposition method to form Pt NPs, and then the good biocompatibility, high specific surface area, high electron transfer efficiency and high enzyme loading of RGO‑CS‑Fc are utilized characteristics, adsorbing a large amount of cholesterolase to construct an enzyme electrode, combined with the catalytic amplification effect of cholesterolase and Pt NPs, cholesterol decomposes and generates H 2 o 2 , and finally according to the detection H 2 o 2 The redox voltammetry current value is drawn, and the working curve is drawn to achieve highly sensitive detection of cholesterol.
Description
technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting cholesterol by constructing an enzyme electrode made of composite materials. Background technique [0002] The technologies currently used to detect cholesterol (Cholesterol) are mainly spectrophotometric, colorimetric, fluorescent, and chemical methods based on enzymatic methods. The invention patent with the publication number CN108776163A discloses a screen-printed electrode and a material recognition enzyme film (made of cholesterol oxidase, cholesterol esterase, polyvinyl alcohol, chitosan, carbon, etc.) adsorbed on the surface of the screen-printed electrode. prepared by mixing nanotubes) to construct a cholesterol enzyme biosensor, and then detect cholesterol electrochemically. The invention patent of CN103954660A discloses a cholesterol enzyme biosensor constructed by a classic three-electrode system, wherein a specific sub...
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IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/3272G01N27/3277G01N27/3278G01N27/48
Inventor 李桂银曾俊祥周治德梁晋涛黄勇
Owner GUILIN UNIV OF ELECTRONIC TECH