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Castanea henryi mycorrhizal seedling culture method

A mycorrhizalization and chestnut technology, applied in the field of cultivation, can solve problems such as the impact on the survival rate of afforestation, low soil nutrient content, and low planting survival rate, so as to shorten the time for preparation of bacterial agents, alleviate soil pollution, and improve biological activity Effect

Inactive Publication Date: 2019-08-23
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the chestnuts are good seedlings in the nursery. Transplanting the seedlings will cause root damage, less fine roots, and a long period of afforestation, which has a certain impact on the survival rate of the afforestation in the hilly and mountainous areas of the chestnut.
At the same time, in chestnut orchards with poor soil fertility, the soil nutrient content is very low, and it is difficult to fertilize
Therefore, how to solve the problem of low survival rate of seedlings in the afforestation of Chinese chestnut varieties in hilly and mountainous areas has become an urgent problem to be studied and solved.

Method used

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  • Castanea henryi mycorrhizal seedling culture method
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  • Castanea henryi mycorrhizal seedling culture method

Examples

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preparation example Construction

[0044] Culture medium preparation

[0045] MMN medium: (NH 4 ) 2 HPO 4 0.25g / L, KH 2 PO 4 0.5g / L, NaCl 0.025g / L, MgSO 4 ·7H 2 O0.15g / L, CaCl 2 0.05g / L, Thiamine HCl 0.1mg / L, FeCl 3 ·6H 2 00.02g / L, Malt Extract 3g / L, Glucose 10g / L, pH 5.5-5.7.

Embodiment 1

[0047] refer to figure 1 , a method for mycorrhizal seedling cultivation of Chestnut Chestnut, comprising the steps of:

[0048] S1, cultivating chestnut seedlings

[0049] Use carbendazim to sterilize and disinfect the river sand, and keep the river sand at a certain humidity, that is, knead it into a ball, loosen it after letting go; water selection to remove the hollow chestnut seeds, diseases and insect pests, use 0.5% carbendazim The solution is soaked and disinfected for 10 to 15 minutes. Sow the seeds of the chestnut horizontally, cover the river sand with a thickness of about 5-8cm, one layer of river sand and one layer of seeds. After the sowing is completed, put them in an artificial climate chamber and cultivate them for 3-4 months, and the growth conditions of the chestnuts are basically the same. seedling.

[0050] S2, preparation of solid bacterial agent

[0051]Inoculate the hyphae of boletus savory strains in MMN medium, and cultivate them at 25°C until the...

Embodiment 2

[0057] A method for cultivating mycorrhized seedlings of Chestnut, comprising the steps of:

[0058] S1. Cultivate chestnut seedlings

[0059] Use carbendazim to sterilize and disinfect the river sand, and keep the river sand at a certain humidity, that is, knead it into a ball, loosen it after letting go; water selection to remove the hollow chestnut seeds, diseases and insect pests, use 0.5% carbendazim The solution is soaked and disinfected for 10 to 15 minutes. The seeds of the chestnut are sown horizontally, the thickness of the covered river sand is about 5-8cm, one layer of river sand and one layer of seeds. After the sowing is completed, they are placed in an artificial greenhouse and cultivated for 3-4 months, and the seedlings of the chestnut with basically the same growth condition are obtained. .

[0060] S2, preparation of solid bacterial agent

[0061] Inoculate the hyphae of the colored puffball species in MMN medium and cultivate at 25°C until the mycelia co...

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Abstract

The invention relates to a castanea henryi mycorrhizal seedling culture method which includes the steps: S1 castanea henryi seedling culture: sowing disinfected castanea henryi seeds into river sand and performing culture to obtain castanea henryi seedlings; S2 solid microbial agent preparation: culturing ectomycorrhizal fungi in an MMN medium to obtain an activated bacteria plate, picking activated bacteria blocks, inoculating the activated bacteria blocks into the MMN liquid medium and performing culture to obtain liquid microbial agents, inoculating the liquid microbial agents into a sterilization solid medium and performing culture to obtain solid microbial agents; S3 inoculation treatment: partially cutting root systems of the castanea henryi seedlings, planting the castanea henryi seedlings into substrates, burying the solid microbial agents near the root systems of the castanea henryi seedlings and performing inoculation treatment. According to the castanea henryi mycorrhizal seedling culture method, the solid microbial agents are inoculated after castanea henryi roots are cut, so that the root systems of the cultured seedlings more effectively coexist with root bacteria, the problems of few fine roots of seedling culture on a castanea henryi nursery land and underdevelopment of the root systems are solved, and the survival rate of transplanted seedlings is greatly increased.

Description

technical field [0001] The invention relates to the field of cultivation, in particular to a method for mycorrhized seedling cultivation of Chestnut chestnut. Background technique [0002] Castanea herryi belongs to the genus Chestnut in the family Fagaceae, and is an important woody food tree species endemic to my country. Nuts are rich in nutrients, rich in starch, vitamin C, trace elements easily absorbed by the human body and other components, and are called "forest food". The fruit can be made into chestnut powder or canned. The wood and bark of the shell contain a lot of tannins, which can be used to extract tannins. Chestnut has a solid material and is resistant to water and humidity. It is an economical species of fast-growing high-quality timber for sleepers, construction, shipbuilding, and furniture. Chestnut is mainly distributed in the south of the Yangtze River Basin in my country, with an existing planting area of ​​1 million hectares 2 , with an annual outp...

Claims

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Application Information

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IPC IPC(8): A01G17/00A01G18/10A01G9/029
CPCA01G17/005A01G9/0299A01G18/10
Inventor 邹锋杨颖慧熊欢金亦佳袁德义陈王尊陈萍田诗义
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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