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Influenza vaccine liposome modified by polyethylene glycol and preparation method of influenza vaccine liposome

A polyethylene glycol, influenza vaccine technology, applied in the directions of liposome delivery, biochemical equipment and methods, pharmaceutical formulations, etc., can solve problems such as technical solutions that have not yet been seen, and achieve strong cellular immunogenicity and simple preparation process. , the effect of easy industrialization

Pending Publication Date: 2019-08-23
KUNMING MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Upon inquiry, before the application was filed, no technical solution identical to the application had been seen

Method used

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  • Influenza vaccine liposome modified by polyethylene glycol and preparation method of influenza vaccine liposome
  • Influenza vaccine liposome modified by polyethylene glycol and preparation method of influenza vaccine liposome
  • Influenza vaccine liposome modified by polyethylene glycol and preparation method of influenza vaccine liposome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The influenza vaccine liposome preparation prescription 1 of embodiment 1PEG modification:

[0065]

[0066] Prescription 2:

[0067]

[0068] Prescription 3:

[0069]

[0070] Prescription 4:

[0071]

[0072] Prescription 5:

[0073]

[0074] Prescription 6:

[0075]

[0076] Prescription 7:

[0077]

[0078]

[0079] Prescription 8:

[0080]

[0081] Prescription 9:

[0082]

[0083] Prescription 10:

[0084]

[0085] Prescription 11:

[0086]

[0087] Prescription 12:

[0088]

[0089] Prescription 13:

[0090]

[0091] Prescription 14:

[0092]

[0093]

[0094] Prescription 15:

[0095]

[0096] Prescription 16:

[0097]

[0098] Prescription 17:

[0099]

[0100] Prescription 18:

[0101]

[0102] The preparation method of the polyethylene glycol-modified influenza liposome vaccine of prescription 1 to prescription 18 in the present embodiment is as follows:

[0103] (1) Disperse the chole...

Embodiment 2

[0110] Example 2 Preparation of Unmodified Influenza Vaccine Liposomes

[0111] Prescription 19:

[0112]

[0113] The preparation method of the unmodified influenza liposome vaccine of prescription 19 in this example is consistent with that in Example 1, except that the step of adding PEG is not included in the whole preparation process. The prepared unmodified influenza liposome vaccine will be used for animal immunogenicity experiment and stability evaluation.

Embodiment 3

[0114] Embodiment 3 animal immunogenicity experimental research

[0115] 1. Animal grouping and immunization

[0116] The mice were randomly divided into a polyethylene glycol-modified influenza vaccine liposome group, a PBS blank control group, a vaccine stock solution group, and an unmodified influenza vaccine liposome group, with 3 mice in each group. Among them, PBS is the negative blank control group, and the vaccine stock solution is the positive control group. The dose of the vaccine administration group was calculated as 6 μg per mouse based on HA. After anesthesia, one of pulmonary administration immunization and intraperitoneal administration immunization was used for immunization on 0d, and immune-related indicators were detected on 7d, 14d, and 28d thereafter. Wherein the vaccine used in the polyethylene glycol modified influenza vaccine liposome group is prepared according to prescription 2 and prescription 8 in Example 1; the vaccine used in the unmodified infl...

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Abstract

The invention provides an influenza vaccine liposome modified by polyethylene glycol and a preparation method of the influenza vaccine liposome. The vaccine liposome is prepared from an influenza virus cracking inactivated vaccine, phospholipid, cholesterol and polyethylene glycol. The method comprises the steps of adopting cholesterol and phospholipid for preparing the liposome, adding polyethylene glycol as a hydrophilic surface modifier, and preparing the influenza vaccine liposome modified by polyethylene glycol through a freeze thawing-lyophilization method. The influenza vaccine liposomehas the advantages of simple preparation method, great stability, low cost, high immunity efficiency and the like.

Description

[0001] Technical field: [0002] The invention belongs to the field of biotechnology preparations, in particular to the field of vaccine biological preparations, and mainly relates to a polyethylene glycol-modified influenza vaccine liposome and a preparation method thereof. [0003] Background technique: [0004] Influenza (influenza, referred to as flu) is an acute respiratory infectious disease caused by influenza virus that threatens human physical and mental health. Today, even with vastly improved preventive measures and medical technology, seasonal influenza causes 3-5 million severe cases and 300,000-500,000 deaths worldwide each year, according to the World Health Organization. And China is a country where influenza viruses frequently occur. Since 1957, the three world pandemic influenzas have all originated from my country. The frightening thing about influenza is that it generally does not directly lead to the death of the patient, but it can cause many serious heal...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/10A61K39/145A61P31/16
CPCA61K9/1271A61K39/12A61K47/10A61K2039/5252A61P31/16C12N2760/16234
Inventor 鲁卫东刘馨普梦笛方国良佘振南王海垠逯荻范雅婷
Owner KUNMING MEDICAL UNIVERSITY
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