Recombinant bacillus calmette guerin vaccine for toxoplamasis and preparation method thereof
A technology of recombinant BCG and Toxoplasma gondii, which is applied in the field of preparation of Toxoplasma recombinant BCG vaccine, can solve the problems of weak anti-infection ability and inapplicability to human beings, and achieve easy transportation and storage, strong humoral immune adjuvant effect, thermal good stability effect
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[0025] Preparation of Toxoplasma gondii recombinant BCG vaccine of the present invention
[0026] Taking the protective antigen Cyclophilin gene of Toxoplasma gondii as an example, the shuttle expression vector and the integrated expression vector were constructed.
[0027] 1. The preparation steps of the recombinant BCG vaccine of the shuttle expression vector Toxoplasma gondii:
[0028] According to the Cyclophilin gene DNA sequence and the physical map of the shuttle vector pMV261, two pairs of primers were designed and restriction restriction sites were introduced.
[0029] Upstream primer QF1: 5'- GAATTCATGAAGCTCGTGCTGTTTTTCCT -3'; the 5' end contains an EcoRI site;
[0030] Downstream primer QR: 5'- GTCGACTTACTCCAACAAACCAATGTCCGT -3'; the 5' end contains a SalI site.
[0031] The PCR purified product was cloned into the pMD18-T vector and identified by PCR, enzyme digestion and sequencing. The fragment recovered from the gel was ligated with the shuttle expression vect...
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