Microporous polymer coated hydrophilic resin and application thereof in glycopeptide enrichment

A technology of hydrophilic resin and microporous polymer, which is applied in the field of preparation of hydrophilic resin, can solve the problems of non-glycopeptide interference and low signal of glycopeptide, and achieve the synthesis route with fewer steps, cheap reaction conditions, and the preparation process green flexible effect

Active Publication Date: 2019-08-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, glycopeptide signals in real samples are usually low a...

Method used

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  • Microporous polymer coated hydrophilic resin and application thereof in glycopeptide enrichment
  • Microporous polymer coated hydrophilic resin and application thereof in glycopeptide enrichment
  • Microporous polymer coated hydrophilic resin and application thereof in glycopeptide enrichment

Examples

Experimental program
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Embodiment 1

[0029] (1) Prepare porous epoxy resin microspheres by seed swelling method, as follows:

[0030]Solution A: Prepare 1 L of absolute ethanol solution containing 20 g / L of polyvinyl alcohol and 20 g / L of non-ionic surfactant Triton X-100. Solution B: prepare 16 mL of 3 g / L azobisisobutyronitrile in styrene. Under a nitrogen atmosphere, slowly add solution B to 64mL solution A, stir the resulting mixed system at room temperature at a mechanical stirring speed of 220r.p.m. for 1 hour, then raise the temperature to 70°C, and polymerize for 12 hours to obtain The product polystyrene microspheres were washed three times with absolute ethanol and water successively, and then vacuum-dried at 60° C. for 12 hours before use. Prepare 1 L of aqueous solution containing polyvinyl alcohol as 10 g / L and sodium lauryl sulfate as 2.5 g / L as the water phase solution of the epoxy resin reaction system prepared by seed swelling polymerization. First, add 0.45g of polystyrene microspheres into 15...

Embodiment 2

[0055] (1) Adopt seed swelling method to prepare porous epoxy resin microspheres, concrete steps are with embodiment 1 step (1)

[0056] (2) The preparation of the hydrophilic resin coated with microporous polymers, the specific steps are the same as the step (2) of Example 1, the difference is: the mass of isophthalaldehyde dropped in is reduced to 38mg, i.e. isophthalaldehyde and The molar ratio of m-phenylenediamine was 0.8.

[0057] (3) The product characterization method is the same as (3) in Example 1. The difference between the obtained results is that the specific surface area is 120m 2 / g, the water contact angle of MAR@MOP is 27°.

[0058] (4) Enzymatic hydrolysis of protein samples and (5) application of hydrophilic resin to enrich glycopeptides in protein hydrolyzate are the same as (4) and (5) in Example 1.

[0059] When directly performing mass spectrometry analysis on the IgG enzymatic hydrolysis solution, the mass spectrogram was dominated by non-glycopeptid...

Embodiment 3

[0061] (1) Adopt seed swelling method to prepare porous epoxy resin microspheres, concrete steps are with embodiment 1 step (1)

[0062] (2) The preparation of the hydrophilic resin coated with microporous polymers, the specific steps are the same as the step (2) of embodiment 1, the difference is: the m-phthalaldehyde quality of dropping is reduced to 24mg, that is, m-phthalaldehyde and The molar ratio of m-phenylenediamine was 0.5.

[0063] (3) The product characterization method is the same as (3) in Example 1. The difference between the obtained results is that the specific surface area is 100m 2 / g, the water contact angle of MAR@MOP is 30°.

[0064](4) Enzymatic hydrolysis of protein samples and (5) application of hydrophilic resins to enrich glycopeptides in the enzymatic hydrolyzate of proteins are the same as steps (4) and (5) in Example 1.

[0065] When mass spectrometry was performed directly on the IgG enzymatic hydrolysis solution, the mass spectrogram was domi...

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Abstract

The invention specifically relates to preparation of a microporous polymer coated hydrophilic resin and application thereof in glycopeptide enrichment. The preparation method includes: firstly preparing porous epoxy resin microspheres that have a particle size of 8-15microm and contain a plurality of epoxy functional groups on the surfaces, then adding m-phenylenediamine and isophthalaldehyde, andtaking acetic acid as the catalyst to prepare the hydrophilic resin coated with a layer of microporous organic polymer on the surface. The preparation conditions of the hydrophilic resin are simple and the reaction is mild. And finally, a standard glycoprotein (IgG) enzymolysis liquid is adopted as the sample to investigate the enrichment performance of hydrophilic resin to glycopeptide, and thehydrophilic resin can be further applied to glycoproteomics analysis in rat liver.

Description

technical field [0001] The invention specifically relates to the preparation of a microporous polymer-coated hydrophilic resin, which can be used for glycopeptide enrichment in the field of life and health. Background technique [0002] Protein glycosylation is one of the important post-translational modifications, which plays a very important regulatory role in many biological processes (document 1. Yan et.al "Selective enrichment of glycopeptides / phosphopeptides using porous titania microspheres", "Chemical Communications", 2010, 46(30), 5488-5490). Glycoprotein abnormalities are closely related to a variety of human diseases, so the detection and identification of glycoproteins have important guiding significance for the diagnosis and treatment of diseases. At present, in the analysis of glycoproteins and glycopeptides, mass spectrometry is used because of its high sensitivity and resolution (document 2. Dell et.al. "Glycoprotein structure determination by mass spectrome...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30B01J13/22B01J13/20B01J13/14C07K1/22C08J9/28C08J9/40C08F112/08
CPCB01J13/14B01J13/20B01J13/22B01J20/262B01J20/28021B01J2220/4812C07K1/22C08F112/08C08J9/28C08J9/405C08J2201/0502C08J2201/0504C08J2363/00
Inventor 欧俊杰李亚董靖叶明亮姜利于之渊
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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