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Kit for predicting lung adenocarcinoma progression, prognosis and drug resistance of lung-adenocarcinoma patients using HOXB13 combined with HOXB13 downstream target gene detection

A kit and technology for lung adenocarcinoma, applied in the biological field, can solve problems such as no significant improvement in prognosis, deterioration of the condition, and treatment failure, and achieve good chemotherapy resistance and disease prognosis

Inactive Publication Date: 2019-08-23
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the treatment technology of lung cancer has developed rapidly, it is still difficult to detect early and receive timely treatment for lung adenocarcinoma, the prognosis has not been significantly improved, and the 5-year survival rate of patients is still very low, only about 10%.
Cisplatin combined with paclitaxel or gemcitabine is the first-line chemotherapy regimen for lung adenocarcinoma. However, the effective rate of chemotherapy is only 40%-50%. Most adenocarcinoma patients will develop drug resistance after half a year to one year after treatment. , making the condition worse
Drug resistance and metastasis in lung adenocarcinoma have become the leading causes of treatment failure and patient death

Method used

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  • Kit for predicting lung adenocarcinoma progression, prognosis and drug resistance of lung-adenocarcinoma patients using HOXB13 combined with HOXB13 downstream target gene detection
  • Kit for predicting lung adenocarcinoma progression, prognosis and drug resistance of lung-adenocarcinoma patients using HOXB13 combined with HOXB13 downstream target gene detection
  • Kit for predicting lung adenocarcinoma progression, prognosis and drug resistance of lung-adenocarcinoma patients using HOXB13 combined with HOXB13 downstream target gene detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] In this example, 75 cases of lung adenocarcinoma were collected from lung cancer pathological sections of the Third Hospital of Peking University. Collect complete case information: including file number, name, gender, age, pathological diagnosis (type and degree of differentiation), TNM classification and survival period, etc. The expression level of HOXB13 was detected by immunohistochemical method. To evaluate the differences in the expression of HOXB13 in different stages and grades of lung adenocarcinoma. The result is as figure 1 It showed that high level of HOXB13 was positively correlated with tumor size grade, lymph node metastasis grade and AJCC grade. Kaplan-Meier analysis of the survival difference between the expression group and the non-expression group, the specific detection method is as follows. High HOXB13 expression predicts poor prognosis in lung adenocarcinoma.

[0068] The specific steps of the immunohistochemical method are as follows:

[006...

Embodiment 2

[0081] Cell drug resistance experiments were used to detect the drug resistance of cells with high expression and low expression of HOXB13. The result is as figure 2 As shown, the high expression of HOXB13 is positively correlated with the drug resistance of lung adenocarcinoma cells to cisplatin and paclitaxel, and the high expression makes the cell drug resistance curve shift to the right, that is, the drug resistance ability is enhanced.

[0082] Specific steps are as follows:

[0083] 1) Take the cells of each group in the logarithmic growth phase, digest them with 0.25% trypsin and pipette into a single cell suspension, neutralize with complete culture medium to prepare a single cell suspension, wash with PBS three times after centrifugation, and use complete culture medium resuspension;

[0084] 2) Count the cells and adjust the concentration of the cell suspension to 2×10 4 a / ml;

[0085] 3) Add 100 μl medium to each well of a 96-well plate containing 2000 cells, a...

Embodiment 3

[0090] Tumor formation experiments in nude mice combined with chemotherapy were used to treat tumors in vivo with cisplatin and paclitaxel. The results were as follows: image 3 As shown, high expression enhances drug resistance of cells. High expression of HOXB13 up-regulated the expression of EZH2, ABCG1, Slug and itself. Such as Image 6 As shown in the xenograft model, immunohistochemical detection found that the expression of HOXB13 and EZH2 in the drug-resistant group was higher than that in the sensitive group, and the treatment of cisplatin significantly up-regulated the expression of HOXB13 and EZH2 in the tumor.

[0091] Tumor resistance test in nude mice:

[0092] 1) SPF-grade BALB / c nude mice, 4-6-week-old female mice raised by the SPF-grade Animal Center of Peking University Health Science Center were used. Seven rats in each group, divided into four groups, respectively Flag control group, Flag administration group, Flag-HOXB13 control group and Flag-HOXB13 ad...

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Abstract

The invention provides a kit for predicting the lung adenocarcinoma progression, prognosis and drug resistance of lung-adenocarcinoma patients using HOXB13 combined with HOXB13 downstream target genedetection. The kit includes q-PCR primers of HOXB13, ABCG1, EZH2 and IL6. The kit can be used for effectively detecting HOXB13 and the transcriptional expression levels of the HOXB13 downstream targetgenes (ABCG1, EZH2 and IL6), and the expression sum of the four molecules is used for predicting the drug resistance and disease prognosis of the lung-adenocarcinoma patients. Moreover, the molecularcombination predicts that the discovery of the chemotherapy resistance and the poor prognosis lays a foundation for extensive clinical application of the molecular combination.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a kit for predicting the progression, prognosis and drug resistance of lung adenocarcinoma by combining HOXB13 with its downstream target gene detection. Background technique [0002] The incidence of lung adenocarcinoma is increasing year by year, and it has become the most common histological type among all lung cancers. Although the treatment technology of lung cancer has developed rapidly, it is still difficult to detect early and receive timely treatment for lung adenocarcinoma. The prognosis has not been significantly improved, and the 5-year survival rate of patients is still very low, only about 10%. Cisplatin combined with paclitaxel or gemcitabine is the first-line chemotherapy regimen for lung adenocarcinoma. However, the effective rate of chemotherapy is only 40%-50%. Most adenocarcinoma patients will develop drug resistance after half a year to one year after treatment. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118
Inventor 张宏权战军王鹏
Owner PEKING UNIV
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