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Peanut shell extractive, preparation method and application

A peanut shell and extract technology, applied in biochemical equipment and methods, microorganism-based methods, food ingredients containing natural extracts, etc., can solve the problems of increasing purification difficulty, organic reagent residues, etc. flavor effect

Active Publication Date: 2019-08-27
INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor believes that the above extraction method often requires the addition of organic reagents for extraction, which inevitably results in residual organic reagents and increases the difficulty of subsequent purification.

Method used

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  • Peanut shell extractive, preparation method and application
  • Peanut shell extractive, preparation method and application

Examples

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preparation example Construction

[0025] The second aspect of the present disclosure provides a preparation method of peanut shell extract, the preparation method comprising the following steps: adding the composite bacterial agent described in the first aspect to peanut shell powder, and fermenting to obtain peanut shell extract.

[0026] In some embodiments, the inoculum size of the composite bacterial agent is 10 7 ~10 9 cfu / g.

[0027] In some embodiments, the peanut shell powder is added with a compound bacterial agent and cultured at a constant temperature for 26-30 days to obtain peanut shell extract.

[0028] The third aspect of the present disclosure provides the peanut shell extract provided by the preparation method of the second aspect.

[0029] The fourth aspect of the present disclosure provides the application of the peanut shell extract described in the third aspect as a food antibacterial agent, preservative and / or flavoring agent.

[0030] In some embodiments, the peanut shell extract is u...

Embodiment 1

[0034] The preparation of embodiment 1 composite bacterial agent

[0035] Put Bacillus subtilis with the strain number CICC10023, Bacillus coronoides with the strain number CICC2650, Bacillus amyloliquefaciens with the strain number CICC 10036, and algae-eating bacteria with the strain number CICC 10798 in the MRS respectively. In the culture medium, activate and culture for 36 hours at 37° C. in an aerobic state to obtain a primary activated strain. Select an activated single colony, inoculate it on a new MRS plate medium, and activate and culture it at 37°C for 36 hours in an aerobic state to obtain a secondary activated strain.

[0036] The secondary activated single colonies were selected and placed in liquid MRS medium, and expanded at a constant temperature of 37°C for 24 hours to obtain seed liquids of various strains. The seed solution of the strains was inoculated into the MRS medium at an inoculum amount of 2%, and cultured at a constant temperature of 37° C. for 36...

Embodiment 2

[0038] The activated Bacillus subtilis, Bacillus coronoides and Bacillus amyloliquefaciens bacteria liquids in Example 1 were mixed at a volume ratio of 2:1:1 to obtain composite microbial agent 2.

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Abstract

The invention belongs to the technical field of peanut shell extracting, and particularly relates to a peanut shell extractive, a preparation method and application. The peanut shell, serving as an economic crop by-product, has good use value. In the prior art, a solvent method and a solvent microwave method are mainly used for extracting beneficial ingredients in the peanut shell. The invention provides a method for extracting the beneficial ingredients in the peanut shell by co-culture of the peanut shell and complex microbial inoculants, and a complex microbial inoculant capable of being added into food is obtained through screening. The preparation method is simple, the prepared peanut shell extractive has better inhibitory effect on various bacterial strains, and can be used as a foodpreservative and a bacteriostatic agent.

Description

technical field [0001] The disclosure relates to the technical field of peanut shell extraction, in particular to a peanut shell extract with antibacterial and antiseptic properties and good smell and its application as a food preservative. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Peanut is a kind of nut with abundant output and wide consumption in our country. It can be used to extract edible oil, prepare industrial lubricant and quenching agent, and has good economic value. As a by-product of peanuts, peanut shells also have a wide range of applications: peanut shells contain more carbohydrates and fibers, and can also be used to produce soy sauce, brew liquor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12P39/00C12P1/04C12P1/02C12P7/02A23L3/3472A23L27/10C12R1/125C12R1/645
CPCA23L3/3472A23V2002/00A23L27/10C12N1/14C12N1/20C12P1/02C12P1/04C12P7/02C12P39/00A23V2200/10A23V2250/21
Inventor 王磊李倩范丽霞胥清翠
Owner INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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