Medium for improving antibacterial activity of biocontrol bacillus to plant pathogenic fungi and culture method thereof
A technology of plant pathogenic fungi and bacillus, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problem of the effect and stability of biocontrol bacillus preparations on plant disease control To solve the problems of chemical pesticides, high production costs, and reductions, it can achieve the effect of enhancing the activity of pathogenic bacteria hydrolase, cheap raw materials, and reducing production costs
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Embodiment 1
[0027] 1.1 Strains
[0028] Tested Bacillus: Bacillus subtilis ( Bacillus subtilis ), the deposit number is BNCC110695, purchased from Suzhou Beina Chuanglian Biotechnology Co., Ltd.
[0029] Phytopathogenic fungi: Fusarium oxysporum ( Fusarium oxysporum ), Verticillium dahliae ( Verticillium dahlia ), Botrytis cinerea ( Botrytis cinerea ), Sclerotinia ( Sclerotinia sclerotiorum ), were provided by the Microbiology Laboratory of the School of Oceanography, Huaihai Institute of Technology.
[0030] 1.2 Medium
[0031] Activation medium: beef extract 5g / L, tryptone 10g / L, NaCl 10g / L, agar 20g / L, pH7.0.
[0032] Seed medium: glucose 10g / L, sucrose 8g / L, tryptone 11.5g / L, L-sodium glutamate 3g / L, KCl0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, KH 2 PO 4 1.5g / L, FeSO 4 ·7H 2 O 0.15mg / L, MnSO 4 ·H 2 O 4.5mg / L, biotin 0.28mg / L, candolol 1.3mg / L, pH7.0.
[0033] Fermentation medium: potato 200g / L, sucrose 17g / L, corn yellow syrup 15g / L, L-sodium glutamate 1.5g / L, KH 2 PO 4 2...
Embodiment 2
[0041] 2.1 The effect of the concentration of corn yellow pulp on the antibacterial activity of Bacillus subtilis
[0042] According to the method provided in Example 1, Bacillus subtilis seed liquid was respectively inoculated in the fermentation medium containing 0, 5, 10, 15, and 20 g / L corn yellow pulp for fermentation and cultivation, and sterile water was used to dilute the obtained fermentation after fermentation. solution, so that the cell concentration reached 1 × 10 8 ~1×10 9 CFU / mL, and then carry out antibacterial activity test, the result is shown in the following table 1.
[0043] Corn syrup, also known as corn syrup, is a by-product of corn starch processing and a cheap and easy-to-obtain raw material. Corn yellow pulp is rich in soluble protein, auxin and some precursor substances, which can be used as an organic nitrogen source for microbial growth, and may also promote the biosynthesis of Bacillus bacteriostatic substances. It can be seen from Table 1 that...
Embodiment 3
[0058] According to the method provided in Example 1, the Bacillus subtilis seed liquid is inoculated into these two conventional mediums of LB and nutrient broth respectively to carry out fermentation culture, and after the fermentation is finished, dilute the gained fermentation liquid with sterile water to make the cell concentration reach 1×10 8 ~1×10 9 CFU / mL, and then carry out antibacterial activity determination, the result is shown in the following table 5.
[0059] As can be seen from Table 5, the Bacillus subtilis fermented liquid that adopts substratum fermentation gained of the present invention, to the bacteriostatic rate of these 4 kinds of phytopathogenic fungi of Fusarium oxysporum, Verticillium dahliae, Botrytis cinerea, Sclerotinia sclerotiorum They were 74.9%, 78.0%, 80.6%, and 68.8%, respectively, which were significantly improved compared with conventional medium fermentation. In the culture medium of the present invention, by adding phytic acid, L-prol...
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