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Medium for improving antibacterial activity of biocontrol bacillus to plant pathogenic fungi and culture method thereof

A technology of plant pathogenic fungi and bacillus, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problem of the effect and stability of biocontrol bacillus preparations on plant disease control To solve the problems of chemical pesticides, high production costs, and reductions, it can achieve the effect of enhancing the activity of pathogenic bacteria hydrolase, cheap raw materials, and reducing production costs

Inactive Publication Date: 2019-08-27
江苏牧之歌生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to reports, the effect and stability of the developed biocontrol bacillus preparations on plant disease control have not yet reached the level of chemical pesticides. The main reason is that the microbial activity in the biocontrol preparations is easy to Affected by adverse factors in the surrounding environment and reduced
In addition, compared with chemical pesticides, the production cost of biocontrol live bacteria preparations is relatively high. These factors limit the large-scale application of biocontrol bacillus preparations and gradually replace chemical pesticides.

Method used

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  • Medium for improving antibacterial activity of biocontrol bacillus to plant pathogenic fungi and culture method thereof
  • Medium for improving antibacterial activity of biocontrol bacillus to plant pathogenic fungi and culture method thereof
  • Medium for improving antibacterial activity of biocontrol bacillus to plant pathogenic fungi and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1.1 Strains

[0028] Tested Bacillus: Bacillus subtilis ( Bacillus subtilis ), the deposit number is BNCC110695, purchased from Suzhou Beina Chuanglian Biotechnology Co., Ltd.

[0029] Phytopathogenic fungi: Fusarium oxysporum ( Fusarium oxysporum ), Verticillium dahliae ( Verticillium dahlia ), Botrytis cinerea ( Botrytis cinerea ), Sclerotinia ( Sclerotinia sclerotiorum ), were provided by the Microbiology Laboratory of the School of Oceanography, Huaihai Institute of Technology.

[0030] 1.2 Medium

[0031] Activation medium: beef extract 5g / L, tryptone 10g / L, NaCl 10g / L, agar 20g / L, pH7.0.

[0032] Seed medium: glucose 10g / L, sucrose 8g / L, tryptone 11.5g / L, L-sodium glutamate 3g / L, KCl0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, KH 2 PO 4 1.5g / L, FeSO 4 ·7H 2 O 0.15mg / L, MnSO 4 ·H 2 O 4.5mg / L, biotin 0.28mg / L, candolol 1.3mg / L, pH7.0.

[0033] Fermentation medium: potato 200g / L, sucrose 17g / L, corn yellow syrup 15g / L, L-sodium glutamate 1.5g / L, KH 2 PO 4 2...

Embodiment 2

[0041] 2.1 The effect of the concentration of corn yellow pulp on the antibacterial activity of Bacillus subtilis

[0042] According to the method provided in Example 1, Bacillus subtilis seed liquid was respectively inoculated in the fermentation medium containing 0, 5, 10, 15, and 20 g / L corn yellow pulp for fermentation and cultivation, and sterile water was used to dilute the obtained fermentation after fermentation. solution, so that the cell concentration reached 1 × 10 8 ~1×10 9 CFU / mL, and then carry out antibacterial activity test, the result is shown in the following table 1.

[0043] Corn syrup, also known as corn syrup, is a by-product of corn starch processing and a cheap and easy-to-obtain raw material. Corn yellow pulp is rich in soluble protein, auxin and some precursor substances, which can be used as an organic nitrogen source for microbial growth, and may also promote the biosynthesis of Bacillus bacteriostatic substances. It can be seen from Table 1 that...

Embodiment 3

[0058] According to the method provided in Example 1, the Bacillus subtilis seed liquid is inoculated into these two conventional mediums of LB and nutrient broth respectively to carry out fermentation culture, and after the fermentation is finished, dilute the gained fermentation liquid with sterile water to make the cell concentration reach 1×10 8 ~1×10 9 CFU / mL, and then carry out antibacterial activity determination, the result is shown in the following table 5.

[0059] As can be seen from Table 5, the Bacillus subtilis fermented liquid that adopts substratum fermentation gained of the present invention, to the bacteriostatic rate of these 4 kinds of phytopathogenic fungi of Fusarium oxysporum, Verticillium dahliae, Botrytis cinerea, Sclerotinia sclerotiorum They were 74.9%, 78.0%, 80.6%, and 68.8%, respectively, which were significantly improved compared with conventional medium fermentation. In the culture medium of the present invention, by adding phytic acid, L-prol...

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Abstract

The invention discloses a medium for improving the antibacterial activity of biocontrol bacillus to plant pathogenic fungi, comprising the following components: potato, sucrose, maize paste, sodium L-glutamate, phosphate, MgSO4.7H2O, CaCl2, ferric citrate, MnSO4.H2O, phytic acid, L-proline, threonine, tetramethyl glutaric aciduria, gamma-aminobutyric acid, sodium nitroprusside, hydrocortisone, LaCl3, amino-oligosaccharin, chamomile leach liquor, and celery juice. A biocontrol bacillus seed solution is inoculated into the medium for temperature-variable fermentation culture, so as to promote the growth and reproduction of bacillus, and effectively stimulate the activity of bacillus, enhance the secretion of bacteriostatic substances and the activity of pathogen hydrolase, thereby significantly improving the antibacterial activity of bacillus to plant pathogenic fungi such as fusarium oxysporum, verticillium dahlia, botrytis cinerea, sclerotinia sclerotiorum, and providing a theoreticalbasis for the further development and application of biocontrol bacillus.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a culture medium and a culture method for improving the antibacterial activity of biocontrol bacillus against plant pathogenic fungi. Background technique [0002] my country is a large agricultural country, and plant diseases are one of the main factors affecting agricultural production. There are many types of plant diseases, which can be divided into two categories according to the types of pathogens: 1) Non-infectious diseases: caused by abiotic, such as lack of nutrients, insufficient or excessive water, low-temperature freezing damage and high-temperature burning , unreasonable use of fertilizers and pesticides, or phytotoxicity and poisoning caused by waste water and waste gas; 2) Infectious diseases: caused by organisms, infectious, and various pathogens, such as fungi, bacteria, viruses, nematodes or Seed plants etc. Infectious diseases are often more harmful ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCC12N1/20A01N63/00
Inventor 贺静莹李颖
Owner 江苏牧之歌生态农业科技有限公司
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