Carbon dot inner-filter effect of fluorescence-based Kanamycin detection method

A technology of kanamycin and carbon dot fluorescence, which is applied in fluorescence/phosphorescence, food testing, measuring devices, etc., and can solve problems such as cumbersome operation, low detection limit, and time-consuming

Inactive Publication Date: 2019-08-27
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have the advantages of low detection limit and high sensitivity, there are still some problems, such as time-consuming, high cost, cumbersome and difficult operation, etc.

Method used

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  • Carbon dot inner-filter effect of fluorescence-based Kanamycin detection method
  • Carbon dot inner-filter effect of fluorescence-based Kanamycin detection method
  • Carbon dot inner-filter effect of fluorescence-based Kanamycin detection method

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Effect test

Embodiment 1

[0042] Embodiment 1 of the present invention: a kind of kanamycin detection method based on carbon dot fluorescent inner filter effect, comprises the following steps:

[0043] A: Draw a standard curve of kanamycin concentration and fluorescence intensity change, the standard curve is attached image 3 shown, which includes the following steps:

[0044] A1: Prepare a test sample solution with known kanamycin concentration to obtain product A1. The specific method is: take 16 1.5mL graduated centrifuge tubes, add 10.0 μL of a known concentration of kanamycin standard solution and 10.0 μL of a kanamycin aptamer solution with a concentration of 30 nM to each of the graduated centrifuge tubes, Mix well and incubate at 25°C for 15min, then add 100.0μL of 3.2nM nano-gold AuNPs solution, mix well and incubate at 25°C for 15min, then add 30.0μL of 30μM NaCl salt ion Solution, the solution was fully mixed and incubated at 25°C for another 35min, then added 20.0μL carbon dot CDs soluti...

Embodiment 2

[0051] Embodiment 2 of the present invention: a kind of kanamycin detection method based on carbon dot fluorescent inner filter effect, comprises the following steps:

[0052] A: Draw a standard curve of kanamycin concentration and fluorescence intensity change, the standard curve is attached image 3 shown, which includes the following steps:

[0053] A1: Prepare a test sample solution with known kanamycin concentration to obtain product A1. The specific method is: take 16 1.5mL graduated centrifuge tubes, add 10.0 μL of a known concentration of kanamycin standard solution and 10.0 μL of a kanamycin aptamer solution with a concentration of 30 nM to each of the graduated centrifuge tubes, Mix well and incubate at 30°C for 10min, then add 100.0μL of 3.2nM nano-gold AuNPs solution, mix well and incubate at 30°C for 10min, then add 30.0μL of 30μM NaCl salt ion Solution, the solution was fully mixed and incubated at 30°C for another 30min, then 20.0μL carbon dot CDs solution wit...

Embodiment 3

[0060] Embodiment 3 of the present invention: a kind of kanamycin detection method based on carbon dot fluorescent inner filter effect, comprises the following steps:

[0061] A: Draw a standard curve of kanamycin concentration and fluorescence intensity change, the standard curve is attached image 3 shown, which includes the following steps:

[0062] A1: Prepare a test sample solution with known kanamycin concentration to obtain product A1. The specific method is: take 16 1.5mL graduated centrifuge tubes, add 10.0 μL of a known concentration of kanamycin standard solution and 10.0 μL of a kanamycin aptamer solution with a concentration of 30 nM to each of the graduated centrifuge tubes, Mix well and incubate at 35°C for 5min, then add 100.0μL of 3.2nM nano-gold AuNPs solution, mix well and incubate at 35°C for 5min, then add 30.0μL of 30μM NaCl salt ion Solution, the solution was fully mixed and then incubated at 35°C for another 25min, then added 20.0μL carbon dot CDs sol...

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Abstract

The invention discloses a carbon dot inner-filter effect of fluorescence-based Kanamycin detection method. According to the method, to-be-detected sample solution A1 with a known Kanamycin concentration is prepared; blank control solution A2 is prepared; the A1 and the A2 are respectively scanned by using a fluorophotometer to measure fluorescence intensity corresponding to emission peaks of the solution at 430 nm, so as to obtain fluorescence emission spectrums of the solution; standard curves of variations of the Kanamycin concentration and the fluorescence intensity are drawn according to the fluorescence emission spectrums; a regression equation related to a mutual relationship between the Kanamycin concentration and the fluorescence intensity is established according to the standard curves; to-be-detected solution C with an unknown Kanamycin concentration is prepared; and the C is scanned by using the fluorophotometer to measure fluorescence intensity corresponding to an emissionpeak of the solution at 430 nm, a fluorescence intensity difference between the C and the A2 is calculated, and the difference is substituted in the linear regression equation to obtain a concentration of Kanamycin in the C. The method has the advantages of being convenient to construct, high in sensitivity and good in specificity.

Description

technical field [0001] The invention relates to a kanamycin detection method, in particular to a kanamycin detection method based on carbon dot fluorescent inner filter effect. Background technique [0002] Kanamycin is an aminoglycoside antibacterial antibiotic that has been widely used in veterinary medicine to treat infections such as sheep, mastitis, pneumonia, and bacterial diarrhea. However, the use of kanamycin may produce residues in food, which poses a serious threat to human health, such as tinnitus, deafness, respiratory failure, nephrotoxicity, and allergic reactions. Therefore, many countries and regions have already introduced animal-derived food , such as the maximum residue limit of kanamycin in milk, the maximum residue limit of kanamycin in the EU is 150μg·kg -1 , China 200 μg kg -1 . In order to realize the detection of kanamycin residues in food samples, a variety of traditional detection methods have been established, including gas chromatography (GC)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/04
CPCG01N21/643G01N33/04G01N2021/6432
Inventor 吴远根王金龙陶菡王雪郦
Owner GUIZHOU UNIV
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