Preparation method of ethylene diamine functionalized carbon quantum dot and application thereof in catechol detection
A technology of carbon quantum dots and quantum dots, which is used in the preparation of carbon nanomaterial fluorescent probes and the detection of organic pollutants to achieve high selectivity, anti-interference, high sensitivity and selectivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0036](1) Preparation of ethylenediamine functionalized carbon quantum dots
[0037]0.65g of citric acid and 0.42g of urea were dissolved in 25mL of ethylene glycol, magnetically stirred for 2 hours, heated at 200°C for 10 hours, cooled to room temperature, filtered, dialyzed, and freeze-dried to obtain carbon quantum dot powder. Then add 20 mg of carbon quantum dot powder to 20 mL of MES buffer (pH = 6), disperse ultrasonically for 40 minutes and then blow in nitrogen for 30 minutes to remove the dissolved oxygen in the solution, then place the solution in an ice bath container and Add 120mg 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 60mg hydroxysuccinimide successively, keep the nitrogen atmosphere and use a magnetic stirrer to stir at a constant speed for 12 hours to ensure the reaction get on. Add 250 μL of tert-butoxycarbonyl ethylenediamine under a nitrogen atmosphere and a low temperature of 0°C, and slowly drop a little sodium hydroxide solution (1M) into t...
Example Embodiment
[0040]Example 1
[0041]Inject 1mL of ethylenediamine functionalized carbon quantum dot dispersion (0.1g / L) into a 10mL sample bottle, add 8mL PBS buffer (0.01M, pH=7.4), and inject 1mL catechol solution of different concentrations, After shaking evenly, let it stand at room temperature for 30 minutes, and use a fluorescence spectrometer to perform a fluorescence test.
Example Embodiment
[0042]Example 2
[0043]Inject 1mL of ethylenediamine functionalized carbon quantum dot dispersion (0.2g / L) into a 10mL sample bottle, add 8mL PBS buffer (0.01M, pH=7.4), inject 1mL catechol solution of different concentrations, and shake After uniformity, let it stand at room temperature for 30 minutes, and use a fluorescence spectrometer to perform a fluorescence test.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap