Industrial extracting method of high-content water-soluble centella total glycosides
A technology of centella asiaticosides and its extraction method, which is applied in the field of industrialized extraction of high-content water-soluble asiaticosides, can solve the problems of limited application of asiaticosides and influence on the clarity of asiaticosides, Achieve the effect of improving clarity, reducing production cost and reducing pollution
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[0030] b. Preparation of reference substance solution: Take appropriate amount of madecassoside reference substance and asiaticoside reference substance, weigh them accurately, add methanol to make a solution containing 0.2mg in each 1ml, and obtain it;
[0031] c. Preparation of the test solution: take about 50 mg of this product, accurately weigh it, put it in a 50 ml measuring bottle, add methanol to dissolve it, and dilute to the mark, shake well, filter, and take the filtrate to obtain final product;
[0032] d. Determination method: Accurately draw 10 μl each of the reference substance solution and the test solution, inject it into a liquid chromatograph, measure it, and obtain it.
Embodiment 1
[0034] Take 100 kg of Centella asiatica and put it into a multi-functional extraction tank, add 600 L of 70% alkaline ethanol with pH=8, reflux extraction twice, each time for 2 hours, then combine and concentrate the filtrates to a thermal density of 1.20 (60°C) and collect 160L of water was added to the primary extract, and it was allowed to stand for 19 hours, then the supernatant was taken, and the supernatant was enriched with 50kg of pretreated D101 macroporous resin at a flow rate of 0.5BV At the same time, rinse with water until the color is light to obtain a lotion. When the lotion is neutral, then use 100L of 70% ethanol to elute with a flow rate of 0.5BV / h, collect the eluate, recover ethanol under reduced pressure, and concentrate to hot water. Collect the extract when the density is 1.20 (60°C), and obtain 8.8L of secondary extract, add 44L of ethyl acetate and 440g of activated carbon to the secondary extract, reflux for 30min to decolorize, filter, cool the filtr...
Embodiment 2
[0036] Take 100 kg of Centella asiatica and put it into a multi-functional extraction tank, add 600 L of 70% alkaline ethanol with pH=8, reflux extraction twice, each time for 2 hours, then combine and concentrate the filtrates to a thermal density of 1.21 (60°C) and collect paste, to obtain 24.7L of primary extract; add 150L of water to the primary extract, let it stand for 20 hours, take the supernatant, and use 50kg of pretreated D101 macroporous resin to enrich the supernatant at a flow rate of 0.5BV At the same time, rinse with water until the color is light to obtain a lotion. When the lotion is neutral, then use 100L of 70% ethanol to elute with a flow rate of 0.5BV / h, collect the eluate, recover ethanol under reduced pressure, and concentrate to hot water. When the measured density was 1.21 (60° C.), the extract was collected to obtain 8.2 L of secondary extract, and 37 L of the primary mother liquor after centrifugation in Example 1, 4 L of ethyl acetate and 410 g of a...
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