5-hydroxyindole acetic acid determination reagent

A technology of hydroxyindole acetic acid and reagent, which is applied in the field of 5-hydroxyindole acetic acid determination reagent, can solve the problems of unsuitable detection of 5-HIAA content, small detection scale and high cost, and achieves high-throughput rapid determination, Accurate, less demanding effects

Inactive Publication Date: 2019-08-30
贵州盛世康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are time-consuming, costly, and small-scale, and are not suitable for the detection of 5-HIAA in the urine of clinical patients.
At present, there is a lack of 5-HIAA detection reagents with good stability, high sensitivity and strong specificity in the market, especially good quality automatic detection reagents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The invention relates to a reagent for measuring 5-hydroxyindoleacetic acid, which contains 5-hydroxyindoleacetic acid specific antibody and indicator reagent;

[0033] The 5-hydroxyindoleacetic acid specific antibody is a complete antibody molecule produced after the 5-hydroxyindoleacetic acid immunogen is immunized with experimental animals;

[0034] The indicator reagent is selected from enzyme reagents and radioisotope reagents mixed in a mass ratio of 1:1; wherein the enzyme reagent is composed of 5-hydroxyindoleacetic acid enzyme-labeled conjugates and enzyme substrates, The enzyme-labeled conjugate is glucose-6-phosphate dehydrogenase-hapten enzyme-labeled conjugate, and the substrate of the enzyme is glucose-6-phosphate.

[0035] The complete antibody molecule is a polyclonal antibody obtained by boosting animals with a single 5-hydroxyindoleacetic acid immunogen.

[0036] The preparation method of described 5-hydroxyindoleacetic acid immunogen comprises the st...

Embodiment 2

[0052] The invention relates to a reagent for measuring 5-hydroxyindoleacetic acid, which contains 5-hydroxyindoleacetic acid specific antibody and indicator reagent;

[0053] The 5-hydroxyindoleacetic acid specific antibody is a complete antibody molecule produced after the 5-hydroxyindoleacetic acid immunogen is immunized with experimental animals;

[0054] The indicator reagent is selected from fluorescent reagents and luminescent reagents mixed at a mass ratio of 1:1; wherein the enzyme reagent is composed of 5-hydroxyindoleacetic acid enzyme-labeled conjugates and enzyme substrates, and the enzyme The labeled conjugate is glucose-6-phosphate dehydrogenase-hapten enzyme-labeled conjugate, and the substrate of the enzyme is glucose-6-phosphate.

[0055] The complete antibody molecule is a polyclonal antibody obtained by boosting animals with a single 5-hydroxyindoleacetic acid immunogen.

[0056] The preparation method of described 5-hydroxyindoleacetic acid immunogen comp...

Embodiment 3

[0072] The invention relates to a reagent for measuring 5-hydroxyindoleacetic acid, which contains 5-hydroxyindoleacetic acid specific antibody and indicator reagent;

[0073] The 5-hydroxyindoleacetic acid specific antibody is a complete antibody molecule produced after the 5-hydroxyindoleacetic acid immunogen is immunized with experimental animals;

[0074] The indicator reagent is selected from enzyme reagents and fluorescent reagents mixed in a mass ratio of 1:1; wherein the enzyme reagent is composed of 5-hydroxyindoleacetic acid enzyme-labeled conjugates and enzyme substrates, and the enzyme The labeled conjugate is glucose-6-phosphate dehydrogenase-hapten enzyme-labeled conjugate, and the substrate of the enzyme is glucose-6-phosphate.

[0075] The complete antibody molecule is a polyclonal antibody obtained by boosting animals with a single 5-hydroxyindoleacetic acid immunogen.

[0076] The preparation method of described 5-hydroxyindoleacetic acid immunogen comprises...

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PUM

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Abstract

The invention provides a 5-hydroxyindole acetic acid determination reagent. The 5-hydroxyindole acetic acid determination reagent comprises a 5-hydroxyindole acetic acid specific antibody and an indicator reagent; the 5-hydroxyindole acetic acid specific antibody is a complete antibody molecule generated after an experimental animal is immunized by a 5-hydroxyindole acetic acid immunogen; the 5-hydroxyindole acetic acid immunogen has strong specificity and high immunogenicity, and the prepared anti-5-hydroxyindole acetic acid specific antibody has strong specificity and high titer, and has nocross reaction with common 62 medicines; and according to the invention, a plurality of samples can be simultaneously measured on a full-automatic biochemical analyzer, the high-flux rapid measurementof 5-hydroxyindole acetic acid is realized, the accuracy is high, the specificity is strong, the accuracy and the detection efficiency are greatly improved compared with the prior art, the full automation of the detection process is realized, the requirement on detection personnel is not high, and the 5-hydroxyindole acetic acid determination reagent is easy to realize, popularize and use.

Description

technical field [0001] The invention relates to the field of biological reagents; in particular, it relates to a reagent for measuring 5-hydroxyindoleacetic acid. Background technique [0002] 5-Hydroxyindoleacetic acid (5-HIAA), as a metabolite of 5-hydroxytryptamine (5-HT), is catalyzed by monoamine oxidase through the conversion of 5-hydroxyindole acetaldehyde. 5-HIAA is metabolized in the liver and excreted in the urine. The level of 5-HT is highest at noon and lowest at midnight, so the content of its metabolite 5-HIAA is higher during the day than at night, so the determination of 24h urine can more accurately reflect the level of 5-HIAA. Clinically, elevated levels of 5-HIAA are common in diseases such as carcinoid tumors, tropical sprue, and bronchial oat cell carcinoma, while decreased levels are common in nephropathy, depression, and obsessive-compulsive neurosis. Therefore, the determination of 5-HIAA content in urine can provide an important basis for the diagn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K16/06C07K16/18
CPCG01N33/5306C07K16/18C07K16/06
Inventor 赵海峰蒙凯李俊英李家维
Owner 贵州盛世康生物科技有限公司
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