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Tissue cultivation method of aromatic plant (anaphalis hancockii)

A technology for aromatic plants and tissue culture, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as no tissue culture research reports, and achieve the benefits of multiplication, high speed, and promotion of large-scale production. Effect

Active Publication Date: 2019-09-03
MINZU UNIVERSITY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] People's research on Cymbidium genus has focused on the extraction and analysis of phytochemical components such as Phytochrome japonica, Pearlescent Cymbidium, Milky White Cymbidium, and Cymbidium, but there are no research reports on tissue culture.

Method used

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  • Tissue cultivation method of aromatic plant (anaphalis hancockii)
  • Tissue cultivation method of aromatic plant (anaphalis hancockii)
  • Tissue cultivation method of aromatic plant (anaphalis hancockii)

Examples

Experimental program
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Effect test

Embodiment

[0075] 1. Acquisition of materials

[0076] The materials used in this experiment are the seeds of bell pepper fragrant green, collected from the alpine meadows of 2100-2330m above sea level in Dongling Mountain (40°1'51"N, 115°27'19"E) in Beijing.

[0077] 2. Disinfection of seeds and cultivation of sterile seedlings

[0078] The best disinfection scheme is: alcohol disinfection for 30s, 10% hydrogen peroxide for 15min, and the pollution rate is 0.

[0079] Inoculate the sterilized seeds of the bell pepper in MS medium, and start to germinate about 4 days; about 15 days, the germination rate reaches 98.0%, and each seed grows 2 cotyledons; after culturing to 20 days, 1-2 true leaves are born. ( figure 1 ).

[0080] 3. Induction of callus

[0081] 3.1. Induction effect of 2,4-D

[0082] The leaves and petioles of the 30-day-old seedlings were used as explants. The explants were inoculated into MS medium, 5 per bottle, and 50 explants were inoculated in each treatment, re...

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Abstract

The invention provides a tissue cultivation method of an aromatic plant (anaphalis hancockii). The method includes the following step of a1, cultivating an adventitious bud of the anaphalis hancockiiin a rooting culture medium to obtain a regeneration seedling, wherein the rooting culture medium contains IAA with the concentration of 0.1-2.0 mg / L or IBA with the concentration of 0.1-2.0 mg / L or NAA with the concentration of 0.1-2.0 mg / L. By adding plant growth regulators of specific types and concentrations to culture mediums applied in all the stages of tissue cultivation of the anaphalis hancockii, the reproduction coefficient of the anaphalis hancockii is high, the speed is high, a large number of regeneration seedlings can be obtained within a short period of time, the propagation ofthe anaphalis hancockii is facilitated, and the large-scale production of the anaphalis hancockii is promoted.

Description

technical field [0001] The invention relates to a method for tissue culture of the aromatic plant Bollia chinensis. Background technique [0002] Anaphalis hancockii Maxim is a plant of the genus Anaphalis in the Compositae family. The whole plant has an aromatic smell and is one of the important spice plants. Lingling Xiangqing has high application value, and its whole plant can extract aromatic oil; it can also be used as an ornamental plant, a natural dried flower material, with a fragrance that lasts for several years; it can be used medicinally, the whole plant is used as medicine, and has heat-clearing properties Detoxification effect. In addition, it also has the effect of killing insects. [0003] People's research on Cymbidium genus has focused on the extraction and analysis of phytochemical components such as Phytocymum chrysalis, Pearlescent Cymbidium, Milky Chrysanthemum, and Cymbidium, but there are no research reports on tissue culture. Contents of the inve...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王俊丽郝娜马伟伟黄航君李依杜嘉琳卢娇
Owner MINZU UNIVERSITY OF CHINA
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