A kind of antimicrobial peptide and its application

Abstract

This application discloses an antibacterial peptide in the field of biotechnology. Based on the modification and design of antibacterial peptide VL15, three antibacterial peptides FL15-1, FL15-2, and FL15-3 are obtained, such as SEQ ID NO.1, SEQ ID NO .2. As shown in SEQ ID NO.3, the antimicrobial peptide of the present application has applications in the preparation of broad-spectrum antibacterial drugs for the treatment of Gram-positive bacteria, Gram-negative bacteria, drug-resistant bacteria and fungal infections.

Description

technical field [0001] The invention and the field of biotechnology specifically relate to an antibacterial peptide and its application. Background technique [0002] Antimicrobial peptides (AMPs) are a class of small-molecule active peptides that are encoded by genes and synthesized by ribosomes when organisms defend against the invasion of external pathogens. They are important components of the innate defense system in organisms. As a biologically active small molecule, AMPs generally have antibacterial, fungal, viral or protozoan activities. In addition, AMPs can also be used as drug delivery carriers, antitumor agents, immune modulators, and signaling molecules. [0003] As an important class of clinical drugs, antibiotics have saved countless lives since they were used. However, in recent years, due to the increasing problems of drug abuse, drug residues and bacterial drug resistance, more and more countries have begun to seek alternatives to antibiotics. Antimicrobi...

AI Technical Summary

Problems solved by technology

A considerable number of natural antibacterial peptides have low antibacterial activity, poor stability, high toxicity, or cause hemolysis of eukaryotic cells, etc.

In addition, some antimicrobial peptides have poor inhibitory effects on drug-resistant bacteria and cannot meet the requirements of practical applications; artificial antimicrobial peptides obtained by modifying natural antimicrobial peptides or newly synthesizing them can greatly improve some or even all of the above shortcomings , to suit different application requirements

At present, there are as many as thousands of artificial antimicrobial peptides, such as the artificially synthesized antimicrobial peptide VL15, whose amino acid sequence is VLNRLFDKIRQVIRK (SEQ ID NO.4). It is 1898.35Da, and the isoelectric point is 11.72. However, by analyzing the antibacterial properties of the antibacterial peptide, it is found that the antibacterial effect of the antibacterial peptide is relatively poor. peptide

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