Preparation method of hemoglobin conjugate

A technology for hemoglobin and conjugates, which is applied in the field of preparation of hemoglobin conjugates, can solve problems such as long shelf life, achieve the effects of long shelf life, reduced waste of manpower and material resources, and good economic benefits

Active Publication Date: 2019-09-06
长春市中保牧生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preparation method solves the problem of medication for acute blood loss and hemorrhagic shock, and alleviates the current situation of blood shortage; it does not need to be adapted to the blood type when used, and has a long storage period. Suffering from virus pollution, the product directly fills the gap in the international market; realizes large-scale production

Method used

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  • Preparation method of hemoglobin conjugate
  • Preparation method of hemoglobin conjugate
  • Preparation method of hemoglobin conjugate

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preparation example Construction

[0042] The invention discloses a preparation method of a hemoglobin conjugate, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0043] The reagents or instruments used in the preparation method of the hemoglobin conjugate provided by the present invention can be purchased from the market.

[0044] The filter used in t...

Embodiment 1

[0047] 1. Low temperature centrifugation

[0048] After passing the inspection, the bovine blood enters the biochemical extraction room after being sterilized, puts it into a low-temperature refrigerated centrifuge for five-step low-temperature centrifugation (the temperature is 4°C, and the rotation speed is 4000r / min) after being sterile-weighted, and the relatively pure blood is collected. red blood cells.

[0049] 2. Hypotonic hemolysis

[0050] After weighing, it is pumped into the hemolysis tank in the low temperature room through the pipeline. Paddle propellers are installed in the tank, and the blood cells (pH is 7.4 ± 0.1, osmotic pressure is 130 ± 10mOsm) are added at a uniform speed per unit time. The phosphate solution is used for hemolysis, and the hypotonic solution is added while stirring. The hypotonic solution is pumped into the tank through the liquid inlet on the top of the tank. In the process of hemolysis, in order to reduce the damage to red blood cells...

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Abstract

The invention relates to the technical field of biopharmacy, in particular to a preparation method of hemoglobin conjugate. The preparation method comprises the steps of separating red blood cells, dissolving the red blood cells, separating hemoglobin and cell membrane matrix, purifying by chromatography, carrying out modifying reaction, deactivating viruses, and carrying out ultrafiltration, liquid exchange and chromatography. The preparation method herein helps solve the problem of medication for acute hemorrhage and hemorrhagic shock, and alleviate the current situation of blood source shortage; blood type adapting during use is not required; the hemoglobin conjugate has a long shelf life; the strict virus deactivating technique is used in the preparation process, so that zoonotic infection contamination is avoided; the hemoglobin bridges the international market blank directly; large-scale production is achieved.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a preparation method of a hemoglobin conjugate. Background technique [0002] The biopharmaceutical industry, and even veterinary biological products are relatively weak industries in my country, with great room for development. They are the focus of national support, and the market has demand; the industry needs innovation and urgently needs core technologies. Social changes and environmental impacts lead to serious "blood shortages". Once wars and natural disasters enter extraordinary periods, it will be even worse. This is the case for humans, and animals are no exception. At present, internal bleeding caused by distemper and other diseases in animals is becoming more and more prominent, and the market has no market at all. Therefore, it is urgent to solve the above-mentioned national problems and urgent social problems. Blood substitutes are only researched and pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/805C07K1/36C07K1/34C07K1/18C07K1/107
CPCC07K14/805
Inventor 尚殿明袁书智孟宪伟董玉影何焱
Owner 长春市中保牧生物科技有限公司
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