Method for improving characters of conventional stable diploid rice
A diploid, rice technology, applied in horticultural methods, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of increasing difficulty of target genes, failing to select breeders' phenotypes, disrupting genotypes, etc.
Pending Publication Date: 2019-09-13
WUHAN POLYPLOID BIOTECH CO LTD
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Problems solved by technology
[0003] The disadvantage of this is that the genotype of the original conventional and stable diploid rice A-2X is completely disrupted, and the gene of the B-2X material is also added, which makes it more difficult to select the target gene, and even cannot be selected. The phenotype desired by the breeder and requires 3-4 years of stability
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0022] 1. Induction of autotetraploid rice by using mature homozygous stable diploid rice embryos
[0023] The experimental process of using mature embryos to induce autotetraploid rice can be divided into five steps: (a) seed pretreatment; (b) seed embryo induction of callus; (c) colchicine doubling treatment; (d) differentiation Seedlings are produced to obtain tetraploid plants; (e) rooting and transplanting.
[0024] a. Seed pretreatment: Remove three quarters or more of the endosperm part of the mature rice seed with scissors, leaving the part with the embryo. In the ultra-clean bench, pour the material into the triangular flask, write the serial number, first disinfect the surface with 75% alcohol for about 40 seconds, then wash it with sterilized pure water, add 0.1% mercury liter to soak for about 15 minutes, and soak the During the process, it should be shaken at intervals, and finally washed with pure water for 3-4 times;
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A method for improving characters of conventional stable diploid rice is characterized by comprising steps as follows: (1) homologous tetraploid rice is induced by mature embryos of diploid rice withstable homozygosity; (2) callus is induced by anther of the tetraploid rice, and the tetraploid rice recovers into the diploid rice; the induction process in the step (1) is shown as follows: a, seedpretreatment: 3 / 4 or more of endosperm parts of the mature rice seeds are removed by a pair of scissors, and the parts with the embryos are reserved; in a super clean bench, the material is poured into a triangular flask, the triangular flask is numbered, the material is firstly disinfected with 75% alcohol for about 40 s and then washed clean with sterilized pure water, 0.1% of mercuric chlorideis added, and the material is soaked for about 15 min. The method has the advantages as follows: the character of raw materials A-2X is improved while the genotype of original conventional stable diploid rice A-2X is completely reserved; the diploid rice A1-2X with stable homozygosity after character improvement can be obtained, and the genotype of original A-2X rice is completely reserved.
Description
technical field [0001] The invention relates to the technical field of new crop variety breeding in modern agriculture, in particular to a method for improving the properties of conventional stable diploid rice. Background technique [0002] At present, the most commonly used method for improving the traits of conventional and stable diploid rice breeding materials is hybrid improvement. For example, to improve the traits of conventional and stable diploid rice A-2X, it is necessary to select a B-2X material and Stable diploid rice A-2X is used as female parent or male parent to cross with B-2X material. [0003] The disadvantage of this is that the genotype of the original conventional and stable diploid rice A-2X is completely disrupted, and the gene of the B-2X material is also added, which makes it more difficult to select the target gene, and even cannot be selected. The phenotype desired by the breeder and requires a stable period of 3-4 years. Contents of the inven...
Claims
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Patent Timeline
Login to View More IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/008
Inventor 隗志松舒园园蔡得田王群
Owner WUHAN POLYPLOID BIOTECH CO LTD