Application of carnosol as inhibitor of inflammasomes

A technology of carnosol and inflammasome, applied in the field of drug screening

A technology of carnosol and inflammasome, applied in the field of drug screening

CN110227075AInactive Publication Date: 2019-09-13THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
0 Cites 6 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of carnosol as inhibitor of inflammasomes
  • Application of carnosol as inhibitor of inflammasomes
  • Application of carnosol as inhibitor of inflammasomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] In order to make the purpose, technical solution and advantages of the application clearer, the embodiments of the application will be described in detail below in conjunction with the accompanying drawings. It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined arbitrarily with each other.

[0031] drug:

[0032] Carnosol and MCC950 were purchased from TargetMol; LPS was purchased from Sigma, USA.

[0033] Reagents and materials:

[0034] DMEM medium, fetal bovine serum (U.S. HyClone company); Opti-MEM serum-free medium (U.S. Gibco company); trypsin, double antibody (penicillin-streptomycin solution) (Maichen Technology Beijing Co., Ltd.); adenosine triphosphate ( ATP), nigericin (Nigericin), lipopolysaccharide (LPS), dimethylsulfoxide (DMSO) (Sigma Aldrich, USA); NLRP3, ASC, Caspase-1, pro-Caspase-1, pro-IL1β, GAPDH Antibody (U.S. Cell Signaling Technology Company); CCK-8 k...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of carnosol as an inhibitor of inflammasomes. The carnosol has inhibitory effects on various inflammasomes, is a broad-spectrum inhibitor of inflammasomes, and is used for developing drugs for treating acquired inflammatory diseases and autoimmune diseases.

Description

technical field [0001] This application relates to the field of drug screening, especially but not limited to the use of carnosol as an inhibitor of inflammasomes. Background technique [0002] The inflammasome is a large multiprotein complex in cells composed of receptor protein, apoptosis-associated speck-like protein (ASC) and caspase-specific protease 1 The inflammasome is composed of pro-caspase-1, which recognizes pathogenic microorganisms and endogenous danger signals through pattern recognition receptors (PRR), that is, pathogen-associated molecular patterns (pathogen associated molecular patterns, PAMPs) and danger-related molecules Damage associated molecular pattern (DAMP), recruits and activates caspase-1, cleaves IL-1β and IL-18 precursors, produces corresponding mature cytokines, thereby promoting cell apoptosis, and participates in the body's clearance and adaptability to pathogens Generation of an immune response. The currently discovered inflammasomes are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
13 Sep 2019
Publication
CN110227075A
IPC
A61K31/366; A61P1/04; A61P25/28; A61P3/10; A61P9/10; A61P19/06; A61P1/16; A61P31/04
CPC
A61K31/366; A61P1/04; A61P1/16; A61P3/10; A61P9/10; A61P19/06; A61P25/28; A61P31/04
Inventors
柏兆方; 肖小河