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Method and product of hfn encapsulating platinum drugs

A platinum drug and drug technology, applied in the field of HFn-encapsulated platinum drugs, can solve the problem of no improvement in the toxic and side effects of DDP and CBP, and achieve the effect of reducing the clinical dosage and increasing the encapsulation capacity.

Active Publication Date: 2021-06-08
KUNSHAN XINYUNDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, up to now, the application of DDP and CBP is mainly directly injected with normal saline or 5% glucose diluted and injected intravenously, which has no effect on the toxic and side effects of DDP and CBP

Method used

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  • Method and product of hfn encapsulating platinum drugs
  • Method and product of hfn encapsulating platinum drugs
  • Method and product of hfn encapsulating platinum drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 HFn-carrying DDP process research

[0048] In this example, empty-shell ferritin (HFn, whose sequence is shown in SEQ ID No.1) is used as a carrier, and the inner space of HFn 8nm is used to load cisplatin (DDP, purchased from Dalian Meilun Biotechnology Co., Ltd.), so that DDP To achieve the effect of targeted therapy in tumor treatment, the process uses protein denaturants such as urea and guanidine to treat the protein, and mixes HFn and DDP in a certain proportion in the buffer solution under the corresponding pH conditions and incubates at a specific temperature and time to make DDP successful. Packed into the HFn cavity.

[0049] 1 main equipment

[0050] 1.1 AKTA avant (with G75 desalting column) (GE Healthcare)

[0051] 1.2 constant temperature water bath

[0052] 1.3 Agilent 1260HPLC (with Agilent SEC-5 300A gel exclusion column) (AgilentTechnologies)

[0053] 2. Research on process conditions

[0054] 2.1 HFn-DDP packet data under different temp...

Embodiment 2H

[0104] Example 2 HFn entrapped CBP process research

[0105] This process uses empty-shell ferritin (HFn) as a carrier, and uses the internal space of HFn 8nm to load carboplatin (CBP, purchased from Dalian Meilun Biotechnology Co., Ltd.), so that CBP can achieve the effect of targeted therapy in tumor treatment. The process uses protein denaturants such as urea and guanidine to treat the protein, mixes HFn and CBP according to a certain ratio and incubates at a specific temperature and time, so that CBP is successfully entrapped into the HFn cavity.

[0106] 1. Research on process conditions

[0107] 1.1 HFn-CBP encapsulation data under different temperature conditions

[0108] The constant conditions are: HFn:CBP=2:2 (weight ratio), the buffer solution is pH7.0 20mM PB, the denaturant is 6M urea, the incubation time is 24h, and there is no additive. The results are shown in Table 1.

[0109] Table 1. HFn-CBP entrapment data under different temperature conditions

[0110]...

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Abstract

The invention relates to a method for carrying platinum drugs on HFn and its products. In particular, the present invention specifically explores the optimal concentration of urea or guanidine hydrochloride, different HFn / platinum drugs such as DDP and CBP ratios and / or different incubation conditions on HFn-loaded platinum drugs such as DDP and CBP. conditions were explored. Utilizing the encapsulation conditions obtained in the present invention, HFn can be used to encapsulate platinum drugs such as DDP and CBP conveniently, quickly, efficiently and in sufficient amount.

Description

technical field [0001] The present invention relates to a method for carrying platinum drugs such as DDP (cisplatin) and CBP (carboplatin) on HFn (human H ferritin, i.e. ferritin formed by self-assembly of H subunits of human ferritin) and its products . Specifically, the present invention relates to a method and a product for depolymerizing and refolding HFn by using urea or guanidine hydrochloride and encapsulating platinum drugs such as DDP and CBP. Background technique [0002] Ferritin is an important functional protein that participates in and maintains the balance of iron metabolism. It is a kind of protein with high iron content widely present in animal, plant and microbial cells. From bacteria to humans, although the amino acid sequences of ferritin in different organisms have great differences, their structures are similar. The typical structure of ferritin is composed of two parts: the protein shell and the iron core. The protein shell is a cage structure formed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K47/42A61K33/243A61K31/555A61P35/00
CPCA61K47/6949A61K47/42A61K31/555A61P35/00A61K33/243A61K47/69
Inventor 阎锡蕴范克龙朱京东
Owner KUNSHAN XINYUNDA BIOTECH CO LTD