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Method for reducing heavy metal residue in ganoderma lucidum polysaccharide by membrane method

A technology of Ganoderma lucidum polysaccharides and heavy metals, which is applied in the field of membrane method to reduce heavy metal residues in Ganoderma lucidum polysaccharides, can solve the problems of high maintenance cost, short service life of ion exchange resins, complicated pretreatment, etc., to reduce energy consumption, improve production efficiency, reduce Effect of Solvent Consumption

Active Publication Date: 2019-09-20
华茂(湖州)保健品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The document of CN101367883A discloses a method for extracting Ganoderma lucidum polysaccharide from the fermentation broth. First, the Ganoderma lucidum polysaccharide fermentation broth is pretreated, and some small molecular impurities are removed by ultrafiltration, and then removed by the pretreated cation exchange resin and anion exchange resin. Impurities, the eluent can be obtained after freeze-drying, but the ion exchange resin has problems such as short service life, complicated pretreatment, high maintenance cost, not suitable for large-scale extraction, and the extraction effect is not good

Method used

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  • Method for reducing heavy metal residue in ganoderma lucidum polysaccharide by membrane method
  • Method for reducing heavy metal residue in ganoderma lucidum polysaccharide by membrane method
  • Method for reducing heavy metal residue in ganoderma lucidum polysaccharide by membrane method

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Experimental program
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Effect test

Embodiment 1

[0032] Step 1: Filter the Ganoderma lucidum extract through a primary filter membrane (ceramic membrane, 20nm) to obtain the retentate I and the permeate;

[0033] Step 2: Filter the permeate obtained in Step 1 through a secondary filter membrane (nanofiltration membrane, 2nm) to obtain a retentate II;

[0034] Step 3: Combine Retentate I and Retentate II, concentrate and dry to obtain Ganoderma lucidum polysaccharide product.

Embodiment 2

[0036] Step 1: Select Ganoderma lucidum, wash it with water, slice it, dry it naturally, and grind it for the first time;

[0037] Step 2: Mix the Ganoderma lucidum after initial crushing with water (the ratio of material to liquid is 1g:5ml), microwave treatment for 5min, then ultrasonic treatment for 20min, after placing it at room temperature, centrifugal separation to obtain filtrate and filter residue;

[0038] Step 3: Adjust the pH of the obtained filtrate to 4.0, add cellulase, pectinase, and amylase, hydrolyze at 36°C for 40 minutes, and centrifuge to obtain the Ganoderma lucidum extract. The mass of cellulase is 0.2 times the mass of the filtrate. The amount of peptase is 0.05 times the quality of the filtrate, and the amount of amylase is 0.3 times the quality of the filtrate;

[0039] Step 4: Centrifuge the obtained Ganoderma lucidum extract at high speed (10000r / min, 8min) at room temperature, and collect the supernatant a;

[0040] Step 5: Adjust the pH of the supernatant...

Embodiment 3

[0045] Step 1: Select Ganoderma lucidum, wash it with water, slice it, dry it naturally, and grind it for the first time;

[0046] Step 2: Mix the first crushed Ganoderma with water (the ratio of material to liquid is 1g:20ml), first treat it with microwave for 7 minutes, and then treat it with ultrasonic for 30 minutes, after placing it at room temperature, centrifugal separation to obtain filtrate and filter residue;

[0047] Step 3: Adjust the pH of the obtained filtrate to 4.0, add cellulase, pectinase, and amylase, hydrolyze at 37°C for 30 minutes, and centrifuge to obtain the Ganoderma lucidum extract. The mass of cellulase is 0.8 times the mass of the filtrate. The mass dosage of peptase is 0.02 times the mass of the filtrate, and the mass dosage of amylase is 0.8 times the mass of the filtrate;

[0048] Step 4: Centrifuge the obtained Ganoderma lucidum extract at high speed (10000r / min, 8min) at room temperature, and collect the supernatant a;

[0049] Step 5: Adjust the pH of...

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Abstract

The invention discloses a method for reducing a heavy metal residue in a ganoderma lucidum polysaccharide by a membrane method. The method comprises the following steps: step one: filtering ganoderma lucidum extracting solution by a first-level filter membrane, to obtain trapped fluid I and permeate, wherein the first-level filter membrane is a ceramic membrane of which a pore size is 10-20 nm; step two: filtering the permeate obtained in the step one by a second-level filter membrane, to obtain trapped fluid II, wherein the second-level filter membrane is a nanofiltration membrane of which a pore size is 0.5-2 nm; and step three: merging the trapped fluid I and the trapped fluid II, concentrating and drying, to obtain a ganoderma lucidum polysaccharide product. The method is capable of purifying the ganoderma lucidum polysaccharide through membrane filtration. The technology is capable of synchronously desalting as well as reducing the heavy metal residue, simple in process, simple in device structure, convenient for maintaining, and suitable for industrialization production.

Description

Technical field [0001] The invention belongs to the technical field of ganoderma polysaccharide preparation, and specifically relates to a method for reducing heavy metal residues in ganoderma polysaccharides by a membrane method. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is a large basidiomycete with high nutritional and health value. Since ancient times, Ganoderma lucidum has been regarded as a symbol of auspiciousness, wealth, beauty and longevity. It is also known as "Xiancao" and "Seicao". Traditional Chinese medicine has long been regarded as a precious Chinese herbal medicine for nourishing and strengthening the body and strengthening the body. So far, we have isolated more than 150 compounds from Ganoderma lucidum, which can be divided into polysaccharides, triterpenes, nucleosides, furans, alkaloids, amino acid proteins, sterols, inorganic ions, organic germanium, etc. More than a dozen types of substances, polysaccharides have anti-tumor growt...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 盛云峰谷浩谢中品
Owner 华茂(湖州)保健品有限公司
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