Method for extracting metalloenzymes in active blood cells to prepare anti-nuclear radiation armor

A technology for extracting metal and resisting nuclear radiation, which is applied in the fields of military equipment, aerospace engineering, and biochemical physics, and can solve problems such as the inability to meet the requirements of remanufactured armored products.

Pending Publication Date: 2019-09-20

杨明锋

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AI-Extracted Technical Summary

Problems solved by technology

[0006] In order to overcome the hazards of radiation suffered by commanders and aerospace engineers in the modern battlefield, or the recovery of damage in the radiation zone; and the technical defects that the extraction of metalloenzyme protoplasts cannot meet the requirements of reconstituted armored products, the purpose of the present ...

Abstract

A method for extracting metalloenzymes in active blood cells to prepare an anti-nuclear radiation armor adopts a rapid formation method to ensure the steric configuration original appearance integrity and activity of a product molecular structure and extract the protoplasm of the metalloenzymes to prepare the armor product. The armor product has the advantages: (1) the function of nuclear radiation resistance and the function of a human firewall; (2) the function of rapid recovery from severe fatigue; (3) the function of rapid awakening from severe coma; (4), the function of rapid pain relief and healing in contusion position; (5), the medicinal function of improving the one-clearing and three-resistance functions of a human body; and (6), the function of enriching the power of life operation; the anti-nuclear radiation armor product is the technical equipment filling domestic blanks in modern military battlefield, aerospace engineering and nuclear radiation area.

Application Domain

OxidoreductasesShielding

Technology Topic

ProtoplasmPain relief +11

Examples

Experimental program(4)

Example Embodiment

[0031] Example 1: A method for extracting SOD from animal active blood cells without damaging protoplasts, implementing the following steps and results:

[0032] 1. Collect blood: Take 10kg young and strong animal blood, immediately anticoagulate, cool to 2-5℃ and store;

[0033] 2. Discard the plasma, centrifuge the blood obtained in step ①, discard the supernatant plasma, collect 2.8 kg of active blood cells, discard 7.2 kg of plasma, and store the discarded plasma to prepare prothrombin;

[0034] 3. Purify the blood cells, add the 2.8kg blood cells collected in step ② with the prepared 0.9% saline and add 0.5 times the volume of the same amount of blood cells, mix well, stand for 30 minutes and separate on the centrifuge to reduce the weight of the blood cells by 12% to obtain pure blood cells 2.464kg;

[0035] 4. Preparing agent, mix with 10% ethanol and 90% deionized water, mix well, and cool to 2-5℃ for later use;

[0036] V. Negative pressure ball breaking, mix the 2.464kg purified blood cells obtained in step ③ with the spare preparation in step ④ in an amount of 1:0.5, and put them into the negative pressure ball breaking device, negative pressure 0.5-0.7 kg/force, at the same time heating up 36.3℃, after 10 minutes of stability, increasing to 1.8-1.85kg/force, 30 seconds to relieve pressure to 0, cooling 2-5℃, that is, 3.696kg of mixed SOD solution after ball breaking is obtained;

[0037] 6. Negative pressure purification, put the 3.696kg mixed enzyme solution obtained in step ⑤ into a glass negative pressure container, start the negative pressure 0.2-0.3kg/force, use the nano purifier to collect the SOD solution, discard the ball slag, ibid. , Repeat once, get 2.788kg of pure SOD conventional liquid;

[0038] 7. Degreasing: Mix the SOD conventional liquid obtained in step ⑥ with 1:1 normal saline for 10 minutes, discard 50% of the supernatant, and repeat the 50% of the collected subnatant, which is pure Me Liquid 2.388kg;

[0039] 7. Precipitation, add 2.388kg of pure Me solution obtained in step ⑥ to 2.012kg of acetone of equal volume, cool to 2-5°C, mix well, and let stand for 35 minutes;

[0040] 9. Separation: Put the Me liquid that has been allowed to stand still in step ⑧ into a centrifuge for separation, 3000 rpm, run for 15 minutes, and stop automatically, discard the supernatant to recover copper acetone to obtain Me;

[0041] 10. Freeze-drying: Put the Me obtained in step ⑨ into a freeze dryer at minus 0 53.5°C and freeze-dried into a powder. The finished product is 6.8 grams, and the white is slightly blue. The test result is: activity ratio 9000 U /mg protein, purity 98.41%, overall integrity rate 95.2%, PH value 6.9, yield 1:006.8. Qualified products are stored and armored.

Example Embodiment

[0042] Example 2:

[0043] 1. Take 1kg of blood and obtain 0.58kg of blood cells according to the method of Example ①,

[0044] 2. Follow the steps ③ to ⑨ of Example 1 to obtain Me protoplast that is 10 times less than the result of Example 1.

[0045] The results of the above examples show that the extraction of Me from animal active blood cells has a reasonable design of the process route, the design and manufacture of some self-made equipment are suitable, the original appearance of the molecular structure is complete and ideal, and the technology for small-scale industrial production is reliable.

[0046] The method of making armor in step B of the present invention will be further described below with reference to the embodiments:

Example Embodiment

[0047] Example one:

[0048] 1. Technical standards for detecting metalloenzymes;

[0049] Activity ratio≥8000U/mg

[0050] Purity≥98.2%

[0051] Overall integrity rate ≥95%

[0052] 2. Compatibility of mixed slime: Add the Me product qualified in step ①, add musk 1:0.005 + edible degreasing oil 1:2 to grind the mixed slime for use;

[0053] 3. Forming of armored products: Fill the prepared mixed adhesive body of step ② into the single-sided plastic backing cloth that has been prepared in advance to form the cover, the shape is 52×80.8 cm with rounded corners, and the net content of Me per paste is 10mg;

[0054] 4. Product single packaging: Put the armor formed in step ③ according to the standard into a plastic bag and vacuum seal, one for each bag;

[0055] 5. Vacuum and sterilize: vacuum -0.2-0.3 air pressure is enough, 20 bags of large packaging, UV sterilized storage, room temperature 3-8 ℃ storage, shelf life is five years.

PUM

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