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A method for the determination of n-nitrosamine compounds in meat products using gpc-gc/ms/ms

A technology for nitrosamines and compounds, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of cumbersome, difficult to realize batch sample processing, complicated operation, etc., to achieve convenient operation, achieve efficient batch sample processing, and high sensitivity. Effect

Active Publication Date: 2021-10-29
南通市产品质量监督检验所
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Problems solved by technology

The method standard for the detection of N-nitrosamines is GB 5009.26-2016 "National Food Safety Standard for the Determination of N-nitrosamine Compounds in Food", which involves gas chromatography-mass spectrometry and gas chromatography-thermal energy analysis There are two methods of instrumentation, and the pretreatment processes involved include steam distillation extraction, liquid-liquid extraction purification, concentration treatment, etc. The operation is relatively complicated and cumbersome, and it is difficult to achieve batch sample processing

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  • A method for the determination of n-nitrosamine compounds in meat products using gpc-gc/ms/ms
  • A method for the determination of n-nitrosamine compounds in meat products using gpc-gc/ms/ms
  • A method for the determination of n-nitrosamine compounds in meat products using gpc-gc/ms/ms

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Embodiment

[0057] 1. Standard spectra and curve equations of N-nitrosamine compounds

[0058] Nine kinds of N-nitrosamine compound standard solutions are diluted step by step into 2μg / L, 5μg / L, 10μg / L, 50μg / L, 100μg / L, 200μg / L, 500μg / L series standard solutions, using the present invention The chromatographic conditions and mass spectrometry parameters provided were analyzed, and the total ion current spectra of the standard solutions of 9 kinds of nitrosamine compounds were as follows: figure 1 shown. Taking the concentration of the standard solution as the abscissa, and the corresponding quantitative ion-pair peak area as the ordinate, the corresponding standard curve equations for each compound are obtained. The linear relationship is good, and the correlation coefficients are all greater than 0.99. For details, see Table 1.

[0059] Table 1

[0060]

[0061]

[0062] 2. Analysis of N-nitrosamine compounds in fragrant powder sausage

[0063] Crush the meat product sample, we...

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Abstract

The present invention provides a kind of method utilizing GPC-GC / MS / MS to measure N-nitrosamine compounds in meat products, comprising the following steps: (1) ultrasonic extraction; (2) GPC purification; (3) online concentration; ( 4) GC / MS / MS detection; (5) Qualitative and quantitative analysis. The invention adopts gel permeation chromatography purification, online concentration for sample pretreatment and gas chromatography-tandem mass spectrometry for detection, which is convenient to operate and can realize efficient processing of batch samples. At the same time, it has higher sensitivity and is suitable for the detection of trace components. is of great significance.

Description

technical field [0001] The invention relates to a method for determining N-nitrosamine compounds in meat products by using GPC-GC / MS / MS, in particular to a detection method based on GPC pretreatment and analysis by gas chromatography-triple quadrupole tandem mass spectrometry method. Background technique [0002] N-Nitrosamine compounds are strong carcinogenic substances with teratogenic and mutagenic effects. They have various types and have the typical structure shown in the following formula. They exist in air, soil, water and food in nature. [0003] [0004] The risk of contamination of nitrosamine compounds in meat products, especially those produced by curing, smoking, baking and other processes, is very high, mainly due to the presence of precursor substances such as nitrite, nitrate and amines. Nitrite and nitrate are used as color-preserving agents and preservatives in meat processing, and protein in meat products will decompose to produce amines, which react u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08G01N30/14G01N30/72
CPCG01N30/02G01N30/06G01N30/08G01N30/14G01N30/72G01N2030/062
Inventor 邵彪周小兰陈煜陈刚王琳琳李玲玉沈祖建
Owner 南通市产品质量监督检验所
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