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Preparation method of frozen sections of gonad tissues of penaeus vannamei boone

A technology of frozen section and production method, which is applied in the direction of sample preparation, sampling, and measuring device for testing, which can solve the problems of low efficiency, long time, and poor slicing effect, and achieve time saving, good slicing effect, and high The effect of slicing efficiency

Active Publication Date: 2019-09-24
ZHEJIANG MARICULTURE RES INST
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] The traditional frozen section technology is mainly used to cut fresh tissue, but after the sample is fixed by formaldehyde, paraffin section technology is used for sectioning, which takes a lot of time and is not efficient. Although the efficiency of section staining is high, the section effect is often not good. Therefore, the present invention has developed a method for staining sample sections after formaldehyde fixation, which not only has good efficiency, but also has good staining effect.

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  • Preparation method of frozen sections of gonad tissues of penaeus vannamei boone
  • Preparation method of frozen sections of gonad tissues of penaeus vannamei boone

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0017] A method for making frozen sections of penaeus vannamei gonad tissue, comprising the steps of:

[0018] (1) Dehydration treatment of tissue after formaldehyde fixation: take the ovary tissue of Penaeus vannamei, fix it in 10% formaldehyde solution for later use, then use a scalpel blade to cut out the ovary tissue with a length of 0.5-1.0 cm, soak it in 20% sucrose solution and OTC Frozen embedding agent volume 1:1 liquid, soaking time at least 16 hours;

[0019] (2) Tissue embedding: Take out the ovarian tissue soaked in step (1), put it into a cylindrical hose model containing OTC frozen embedding agent, use tweezers to pick up the model and freeze it in liquid nitrogen for 15-20s , and then put it into the cooled cryostat, remove the model and get the sample, fix the sample on the pre-cooled sample holder with OTC frozen embedding agent, put ...

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Abstract

The invention discloses a preparation method of frozen sections of gonad tissues of penaeus vannamei boone. The preparation method comprises the following steps: tissue dehydrating after formalin fixation, tissue embedding, section freezing, section dyeing and sealing. The method provided by the invention is different from a freezing slicing technology of fresh tissues aiming at a freezing slicing technology of penaeus vannamei boone gonad tissues fixed by formaldehyde. Compared with a traditional paraffin slicing method, the method has higher slicing efficiency, and saves the time. Compared with a common freezing slicing method, the method has a better slicing effect and is beneficial to staining observation.

Description

technical field [0001] The invention relates to a method for making a frozen section, more specifically, it relates to a method for making a frozen section of the gonad tissue of Penaeus vannamei. Background technique [0002] Litopenaeus vannamei, also known as Penaeus vannamei, belongs to the order Deeapoda, the family Penaeidae, and the genus Litopenaeus. It is native to the Pacific coast from Peru to Mexico in Central and South America. It is the leading species in the industry and was introduced into China in 1988 as an alternative species of Chinese prawn (Fenneropenaeus chinensis). Because Litopenaeus vannamei has the advantages of fast growth, short breeding cycle and strong breeding adaptability, the breeding area has expanded rapidly after 2000. It is already one of the pillar industries of aquaculture in China. [0003] The traditional frozen section technology is mainly used to cut fresh tissue, but after the sample is fixed by formaldehyde, paraffin section tec...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/36G01N1/42G01N1/06
CPCG01N1/06G01N1/2813G01N1/286G01N1/30G01N1/36G01N1/42
Inventor 张敏王瑶华李方冀德伟胡利华闫茂仓柴雪良
Owner ZHEJIANG MARICULTURE RES INST
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