Endothelial stem cell bioreactor and preparation method thereof

A bioreactor, stem cell technology, applied in the field of endothelial stem cells, can solve the problems of increasing and decreasing the efficiency of endothelial stem cell culture, endothelial stem cell contamination, etc., and achieves the effects of reducing mortality, reducing injury and maintaining stability

Active Publication Date: 2019-09-27
华夏源细胞工程集团股份有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] However, most of the existing endothelial stem cell reactors use mechanical stirring. The decoupling of the stirring device and the lubricant often pollute the endothelial stem cells inside the reactor, which greatly affects the survival rate of the endothelial stem cells, and The stirring mechanism of mechanical stirring is usually in hard contact with the endothelial stem cells in the reactor, resulting in a significantly increased probability of endothelial stem cell damage or death, resulting in an increase in the number of endothelial stem cell cultures, which virtually reduces the work efficiency of endothelial stem cell culture

Method used

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  • Endothelial stem cell bioreactor and preparation method thereof
  • Endothelial stem cell bioreactor and preparation method thereof
  • Endothelial stem cell bioreactor and preparation method thereof

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Embodiment 1

[0049] see Figure 1-2 , a kind of endothelial stem cell bioreactor, comprising reaction tank 11 and positioning reaction bottom 12, the upper end of positioning reaction bottom 12 is excavated with reaction external movement groove 5, and reaction tank 11 is placed in reaction external movement groove 5, and reaction external movement groove 5 The bottom end is fixedly connected with an airbag 2 with a valve, the airbag 2 is located directly below the reaction tank 11, the inner bottom of the reaction tank 11 is fixedly connected with a fixed stirring column 3, and the outer end of the fixed stirring column 3 is fixedly connected with a plurality of uniformly distributed moving parts. The stirring bar 4 and the external reaction tank 5 are a structure with a large top and a small bottom, and the inner bottom of the external reaction tank 5 is set in a concave spherical shape, so that during the non-stirring stage or when the endothelial stem cells are not cultured, The reacti...

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Abstract

The invention discloses an endothelial stem cell bioreactor and a preparation method thereof and belongs to the field of endothelial stem cells. According to the endothelial stem cell bioreactor and the preparation method thereof, stable and normal cell culture can be kept through matched use of a reaction outer moving groove, double-state positioning bulges and an air bag when stirring is not required; when stirring is required, a reaction tank is lifted, electrification of the double-state positioning bulges is stopped, fixation of the reaction tank is relieved, electromagnets are powered on, the reaction tank is always in a shaking state through repulsive force between the electromagnets and an outer assisting shaking ring, an inside culture is shaken and stirred, movable stirring bars shake along, and further the cultured substances are assisted to be stirred. Compared with the prior art, rigid contact can be turned into flexible contact, damage to cells during stirring is effectively reduced, the death rate of the cells is reduced, the frequency of later cell culture is further reduced, and the work efficiency is improved.

Description

technical field [0001] The invention relates to the field of endothelial stem cells, and more specifically relates to an endothelial stem cell bioreactor and a preparation method thereof. Background technique [0002] Stem cells (gàn) are a type of pluripotent cells with self-renewing ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells (ES cells) and adult stem cells (somatic stem cells) according to their developmental stages. Stem cells are divided into three categories according to their developmental potential: totipotent stem cells (TSCs), pluripotent stem cells (pluripotent stem cells) and unipotent stem cells (unipotent stem cells). Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body. They are called "universal cells" in the medical field. [0003] Simply put, it is a type of primitive ...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/02C12M1/00C12N5/071
CPCC12M23/20C12M23/26C12M23/48C12M27/02C12M27/16C12N5/0692
Inventor 曹梵靖朱灏
Owner 华夏源细胞工程集团股份有限公司
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