Bacillus CCPM7645 with strong anti-cancer activity and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of CCPM7645 and Bacillus, applied in the field of biopharmaceuticals, can solve the problems of large side effects of anticancer drugs and achieve broad-spectrum anticancer activity
Pending Publication Date: 2019-10-01
哈尔滨天齐人类第二基因组技术开发应用科技有限责任公司
View PDF4 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0003] In order to solve the problem of large side effects of existing anticancer drugs, the pr
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0030] A bacillus CCPM7645 with strong anticancer activity belongs to the genus Bacillus and represents a previously unknown bacterial species. CGMCC No.17887, the preservation date is June 3, 2019, and the preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.
Embodiment 2
[0032] This example provides a screening method for Bacillus CCPM7645.
[0033] 1. Preparation method of screening medium and related solutions:
[0034] (1) GRC001 solid medium formula (1L):
[0035] Starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O0.01g, agar 20g; dissolve or suspend starch first, then add other ingredients, shake well after high pressure. The starch in this example is from potato or maize or soybean or millet or other grain or other plant.
[0036] (2) Potassium dichromate: The final concentration of the solution is 50mg / L. The concentration of the stock solution is 5g / L, and the solvent is water. It is prepared immediately after use, protected from light, and sterilized by filtration. Toxic, do not touch the skin or inhale; when preparing 100mL GRC001 solid medium, add 1mL potassium dichromate stock solution, add it when the medium is not too hot, and mix well.
[0037] (3) FeSO 4 ·7H 2 O mother liquor: 0.0...
Embodiment 3
[0046] The present embodiment provides a kind of amplification method of bacillus CCPM7645:
[0047] Take a single colony from the plate into 5mL GRC001 liquid medium, 120 rpm, 14-39°C, amplify for 2-10 days, then transfer 5mL bacterial liquid to 100mL GRC001 liquid medium, 120 rpm, 14-39°C, large-scale amplification for 2-10 days, and finally the bacterial liquid was centrifuged at 8000rpm / min for 10min, and the supernatant was taken for subsequent anti-cancer experiments.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to bacillus CCPM7645 with strong anti-cancer activity and application thereof, and belongs to the technical field of bio-pharmaceuticals. In order to solve the problem that existing anti-cancer medicines have large side effects, the bacillus CCPM7645 with strong anti-cancer activity is provided; the bacillus CCPM7645 belongs to Bacillus, represents a new bacterial species, ispreserved in China Center For Type Culture Collection and has the preservation number of CGMCC No.17887; the bacillus comes from human intestines, is new-source bacillus and can generate metaboliteswith anti-cancer activity, and cancers can be treated by the metabolites of the bacillus. Since the bacillus is found in human intestines, the bacillus has quite small side effects on a human body orhas no side effects on the human body, the problem that the existing anti-cancer medicines have large side effects and poor patient compliance is solved, and the aim of preventing cancers by adjustingthe balance of intestinal flora is expected to be realized.
Description
technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a bacillus CCPM7645 with strong anticancer activity and its application. Background technique [0002] Cancer, as the number one factor that endangers human health, has always been a difficult problem for the world's medical community. Unfavorable changes in the lifestyle of modern people and the emergence of new risk factors are all promoting the occurrence and development of cancer. Traditional cancer treatment methods mainly include radiotherapy and chemotherapy. The drug resistance of cancer cells and the destruction of the body and mind are the obvious shortcomings of this method. Even with the rise of immunotherapy in recent years, it is still immature and there are many unsolved problems, which make it difficult to implement. Finding new ways to fight cancer is imminent. Contents of the invention [0003] In order to solve the problem of large s...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more