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Bacillus CCPM7645 with strong anti-cancer activity and application thereof

A technology of CCPM7645 and Bacillus, applied in the field of biopharmaceuticals, can solve the problems of large side effects of anticancer drugs and achieve broad-spectrum anticancer activity

Pending Publication Date: 2019-10-01
哈尔滨天齐人类第二基因组技术开发应用科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of large side effects of existing anticancer drugs, the present invention provides a bacillus CCPM7645 with strong anticancer activity and its application

Method used

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  • Bacillus CCPM7645 with strong anti-cancer activity and application thereof
  • Bacillus CCPM7645 with strong anti-cancer activity and application thereof
  • Bacillus CCPM7645 with strong anti-cancer activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A bacillus CCPM7645 with strong anticancer activity belongs to the genus Bacillus and represents a previously unknown bacterial species. CGMCC No.17887, the preservation date is June 3, 2019, and the preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

Embodiment 2

[0032] This example provides a screening method for Bacillus CCPM7645.

[0033] 1. Preparation method of screening medium and related solutions:

[0034] (1) GRC001 solid medium formula (1L):

[0035] Starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O0.01g, agar 20g; dissolve or suspend starch first, then add other ingredients, shake well after high pressure. The starch in this example is from potato or maize or soybean or millet or other grain or other plant.

[0036] (2) Potassium dichromate: The final concentration of the solution is 50mg / L. The concentration of the stock solution is 5g / L, and the solvent is water. It is prepared immediately after use, protected from light, and sterilized by filtration. Toxic, do not touch the skin or inhale; when preparing 100mL GRC001 solid medium, add 1mL potassium dichromate stock solution, add it when the medium is not too hot, and mix well.

[0037] (3) FeSO 4 ·7H 2 O mother liquor: 0.0...

Embodiment 3

[0046] The present embodiment provides a kind of amplification method of bacillus CCPM7645:

[0047] Take a single colony from the plate into 5mL GRC001 liquid medium, 120 rpm, 14-39°C, amplify for 2-10 days, then transfer 5mL bacterial liquid to 100mL GRC001 liquid medium, 120 rpm, 14-39°C, large-scale amplification for 2-10 days, and finally the bacterial liquid was centrifuged at 8000rpm / min for 10min, and the supernatant was taken for subsequent anti-cancer experiments.

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Abstract

The invention relates to bacillus CCPM7645 with strong anti-cancer activity and application thereof, and belongs to the technical field of bio-pharmaceuticals. In order to solve the problem that existing anti-cancer medicines have large side effects, the bacillus CCPM7645 with strong anti-cancer activity is provided; the bacillus CCPM7645 belongs to Bacillus, represents a new bacterial species, ispreserved in China Center For Type Culture Collection and has the preservation number of CGMCC No.17887; the bacillus comes from human intestines, is new-source bacillus and can generate metaboliteswith anti-cancer activity, and cancers can be treated by the metabolites of the bacillus. Since the bacillus is found in human intestines, the bacillus has quite small side effects on a human body orhas no side effects on the human body, the problem that the existing anti-cancer medicines have large side effects and poor patient compliance is solved, and the aim of preventing cancers by adjustingthe balance of intestinal flora is expected to be realized.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a bacillus CCPM7645 with strong anticancer activity and its application. Background technique [0002] Cancer, as the number one factor that endangers human health, has always been a difficult problem for the world's medical community. Unfavorable changes in the lifestyle of modern people and the emergence of new risk factors are all promoting the occurrence and development of cancer. Traditional cancer treatment methods mainly include radiotherapy and chemotherapy. The drug resistance of cancer cells and the destruction of the body and mind are the obvious shortcomings of this method. Even with the rise of immunotherapy in recent years, it is still immature and there are many unsolved problems, which make it difficult to implement. Finding new ways to fight cancer is imminent. Contents of the invention [0003] In order to solve the problem of large s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P35/00A61P35/02C12R1/07
CPCA61K35/742A61P35/00A61P35/02C12N1/20C12N1/205C12R2001/07
Inventor 刘树林
Owner 哈尔滨天齐人类第二基因组技术开发应用科技有限责任公司
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