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Screening method of bacterial cellulose synthetic bacteria

Inactive Publication Date: 2019-10-01
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in the mass production of BC, for example, there are few high-yield strains in the bacterial cellulose synthetic bacteria, the screening method is complicated, the time is long, the yield is low, and the production cost is high, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0014] Specific embodiment one: the method for present embodiment screening bacteria cellulose synthetic bacteria is as follows:

[0015] 1. Inoculate naturally rotten grapes in MS medium for enrichment culture, and let them stand for 5-7 days;

[0016] 2. Take the milky white film in the enrichment medium and mash it, squeeze out the bacterial suspension, then apply gradient dilution on the HS separation medium containing fluorescent whitening agent and culture it for 3-5 days;

[0017] 3. Pick fluorescent single colonies under ultraviolet light to isolate pure strains;

[0018] 4. Inoculate the isolated pure strains on MS medium for culture, and select the strains with fast film production time and high yield to complete the screening.

specific Embodiment approach 2

[0019] Embodiment 2: The differences between this embodiment and Embodiment 1 are: the pH value of the culture medium in step 1 enrichment culture is 7.0-7.3, and the culture temperature is 30°C±1°C. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0020] Embodiment 3: The differences between this embodiment and Embodiment 1 or 2 are: in step 2, the pH value of the HS separation medium is 6.0-6.5, and the culture temperature is 30°C±1°C. Other steps and parameters are the same as those in Embodiment 1 or 2.

[0021] In this embodiment, the culture medium adopts a slightly acidic pH value in the stage of separation and purification, and the culture medium adopts a neutral pH value during enrichment, which can effectively remove some non-target bacterial strains.

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PUM

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Abstract

The invention relates to a screening method of bacterial cellulose synthetic bacteria. The screening method of the bacterial cellulose synthetic bacteria is simple, reliable, short in time, easy to operate and low in cost. The method comprises the steps that 1, naturally-rotted grapes are inoculated into a culture medium for enrichment culture; 2, a thin film in an enrichment medium is taken to bemashed and squeezed to obtain bacterium suspension which is applied to a separation culture medium containing a fluorescent whitening agent; 3, pure strains are separated out by means of the luminescent effect of fluorescent whitening agent and cellulose combination; 4, the separated pure strains are inoculated into an MS medium culture for culture, and the strains which are high in membrane production time and high in yield are selected. Accordingly, raw materials come from the grapes rich in strain content, wide, easy to obtain and likely to rot, not only are resources provided for development of bacterium screening work, but also a new idea is provided for turning rotted grape waste into wealth.

Description

technical field [0001] The invention relates to a screening method for bacteria. Background technique [0002] Bacterial cellulose (BC for short) is mainly a kind of high-molecular carbohydrate synthesized by some bacteria outside the cell. Compared with plant fiber, bacterial cellulose does not contain lignin and hemicellulose. Due to its unique synthesis method, bacterial cellulose has an ultra-fine network fiber structure, which is a natural "nano-sponge". And because of its gentle synthesis process and strong film-forming properties, BC membrane is vividly compared to "a piece of non-woven fabric woven with countless bacteria as shuttles". Because of its pure texture, high crystallinity, good mechanical properties, strong water absorption and good biosafety and degradability, BC synthesized by bacteria is recognized as a new type of biomaterial with excellent performance in the world. . As one of the research hotspots of new microbial synthetic materials today, BC has...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02
CPCC12N1/02C12N1/20
Inventor 韩雪容熊健力赵鑫杨家鑫
Owner CHANGCHUN UNIV OF SCI & TECH