Specific sequence and application of citrus sour rot germs

Abstract

The invention belongs to the fields of strain identification and molecular biology, and discloses a specific sequence and application of citrus sour rot germs. On the basis of genomic sequences of published geotricum fungi Geotrichum candidum and Geotrichum fermentans, the whole genome sequence of the citrus sour rot germs and the genomic sequences are subjected to multi-sequence comparison, according to the result, a specific sequence shown in SEQ ID NO.1 is obtained, a primer is designed according to the sequence for PCR amplification, the citrus sour rot germs can be rapidly and accuratelyidentified, whether citrus is infected with the citrus sour rot germs in the citrus storage period or not is predicted, and therefore a theoretical basis is provided for effectively controlling spreading of citrus acid sour rot and prolonging the storage time.

Description

technical field [0001] The invention relates to the fields of strain identification and molecular biology, in particular to a specific sequence and application of a citrus sour rot pathogen. Background technique [0002] Citrus (Citrus reticulata Blanco) is a plant of the Citrus subfamily of Rutaceae (Rutaceae). my country's citrus is mostly planted in mountainous areas, and the ripening period is mostly from October to December. Except for some fresh food for sale, most of the fruits need to be stored for a period of time so that they can be sold in the spring of the next year. However, during the process of citrus harvesting, storage and sales, diseases often cause huge losses. According to statistics, the fruit rot loss during storage and transportation is as high as 10%-15% (Abdel-Aziz and Manssour2006; Izgu et al 2011), even in my country. Reach 20%-30%. According to reports, the main diseases causing this huge loss are green mold, penicillium and acid rot, and these t...

AI Technical Summary

Problems solved by technology

In the past ten years, our research group has carried out many field and scientific experiments on citrus postharvest diseases. The research found that there are the following problems in the storage and preservation of citrus: First, during the storage and preservation of citrus, diseases such as citrus green and green mold have been effectively controlled. , but the acid rot disease is showing an increasing trend year by year; second, there are many citrus postharvest disease fungicides on the market, but only one chemical agent "biguanide salt" has special effects on acid rot bacteria; third, due to long-term use of chemical fungicides Sour rot pathogens in major domestic citrus producing areas have shown obvious drug resistance, so early identification and timely prevention of citrus sour rot bacteria are urgent

The symptoms of sour rot fungus infecting postharvest fruits are similar, and there is no obvious difference in the morphology of the fungi of the same genus. Only the genomes of Geotrichum candidum and Geotrichum fermentans have been sequenced and published, and the identification process is only based on morphological characteristics or physiological characteristics , biochemical indicators, it is difficult to accurately identify different species of sour rot fungi in the genus Geotrichum in a short period of time (Yang Zhuliang, 2013)

In addition, the most widely used modern molecular biology technique in the identification of citrus sour rot is nucleic acid sequence analysis, using conserved sequence primers ITS1 and ITS4 to amplify conserved fragments. Gene conflicts caused by factors such as differences in rates often lead to inaccurate identification results (Gan Senning et al., 2019). In order to be more convenient, quick and accurate in the identification process of citrus sour rot, it will become more important to select specific fragments of conserved genes in the genome as markers. Good choice

Images