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Ophiocordyceps sinensis and its application

A Chinese technology of hairy spores and spores, applied in the field of artificial cultivation of fruiting bodies of Cordyceps sinensis, can solve the problems not mentioned, and achieve the effect of promoting artificial cultivation

Active Publication Date: 2019-10-08
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently published patents and publications do not mention whether the cultivated fruiting bodies can eventually grow ascocarps

Method used

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  • Ophiocordyceps sinensis and its application
  • Ophiocordyceps sinensis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Isolation and identification of Ophiocordyceps sinensis KD11-2 in China

[0019] 1. Acquisition of KD11-2 strain: Fresh Cordyceps sinensis was collected from Yala Township, Lucheng Town, Kangding City, Ganzi Prefecture, Sichuan Province, and transported to our laboratory through cryogenic preservation. In the aseptic operating room or ultra-clean workbench, peel off the soil and bacterial film around Cordyceps sinensis, disinfect the body surface with 75% alcohol; Part of it was cut into small pieces (pay attention to avoid the intestinal tract of the worm), planted on PPDA solid plate medium, and cultured at 9-16°C; the isolated typical colony of Cordyceps sinensis without pollution was confirmed as Cordyceps sinensis after molecular identification , to obtain the strain, and its number as KD11-2.

[0020] 2. Characteristics of KD11-2 strain:

[0021] Morphological characteristics: On the PPDA solid medium, the colony is earthy yellow, the surface of the c...

Embodiment 2

[0028] Experimental location: Guangdong Institute of Applied Bioresources located in Guangzhou City, the following cultivations are all under normal oxygen concentration.

[0029] Put the Mortierella chinensis KD11-2 strain and other Mortierella chinensis strains (KD1223) into the sterile solid PPDA medium respectively, and after culturing in the dark at 9°C for 60 days, pick the colonies and put them into the sterile liquid PPDA medium , at 9°C, 100rpm shaking culture (dark culture) for 65 days, select the fermentation culture with uniform mycelial ball size, 2-3 mm in diameter, and light brown bacterial liquid as a liquid strain, which is used for the production of fruiting bodies of Cordyceps sinensis Cultivated production.

[0030] In a sterile room or an ultra-clean workbench, use sterile water to dilute 5-fold liquid strains (inject 30 mL of diluted liquid strains per 100 g of cultivation medium) into sterile cultivation medium. Place the inoculated culture bottle at 9°C ...

Embodiment 3

[0033] Experimental location: Guangdong Institute of Applied Bioresources located in Guangzhou City, the following cultivations are all under normal oxygen concentration.

[0034] The Mortierella sinensis KD11-2 strain and other Mortierella sinensis strains (KD1223) were respectively inserted into sterile solid PPDA medium, and after dark culture at 16°C for 45 days, the colonies were selected and inoculated into sterile liquid PPDA medium. At 16°C, shake culture at 100 rpm (dark culture) for 40 days, and select the fermentation culture with uniform mycelial ball size and a diameter of 2-3 mm as a liquid strain for the cultivation and production of fruiting bodies of Cordyceps sinensis.

[0035] In a sterile room or ultra-clean workbench, inject 10-fold liquid strains diluted with sterile water (according to every 100 g of cultivation medium, insert 30 mL of diluted liquid strains) into the sterile cultivation medium. Place the inoculated culture bottle at 13°C for dark cultur...

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Abstract

The invention discloses a strain of Ophiocordyceps sinensis and its application. The Ophiocordyceps sinensis KD11-2 has a preservation number of GDMCC No: 60594. The Ophiocordyceps sinensis KD11-2 ofthe present invention can grow sporophore on an artificial medium and form ascocarp (ascospores), and has the outstanding ability to form ascospores, under artificial cultivation condition, the Ophiocordyceps sinensis KD11-2 is used to produce and collect Ophiocordyceps sinensis ascospores, and the artificial cultivation of the Ophiocordyceps sinensis sporophores can be promoted, which provides anew method for utilizing the obtained ascospores to infect the larvae of host Hepialus armoricanus of Cordyceps sinensis.

Description

Technical field: [0001] The invention belongs to the field of artificial cultivation of fruiting bodies of Cordyceps sinensis, and in particular relates to Ophiocordyceps sinensis KD11-2 which can grow fruiting bodies and form ascocarps (ascospores) on artificial culture medium and its application. Background technique: [0002] Cordyceps sinensis is the most distinctive biological resource in my country, belonging to Ascomycota, Sordariomycetes, Hypocreales, Ophiocordycipitaceae, and Ophiocordyceps. In Tibet, Qinghai, Yunnan, Sichuan, Gansu and other snow-capped alpine regions above 3,000 meters above sea level. [0003] Cordyceps sinensis is the larvae of the host insect, the bat moth, which is infected by Trichosporium fungus in China, and makes it ossified. Under suitable conditions, it grows to form a complex structure with worm (zombie) grass (fungi fruiting body). [0004] Cordyceps sinensis has many functions such as "tonifying the lungs, strengthening the kidneys, b...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01H15/00A01G18/40A01G18/20C12R1/645
CPCA01H15/00A01G18/20A01G18/40C12N3/00C12N1/145C12R2001/645
Inventor 曹莉韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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