Ophiocordyceps sinensis and its application

A Chinese technology of hairy spores and spores, applied in the field of artificial cultivation of fruiting bodies of Cordyceps sinensis, can solve the problems not mentioned, and achieve the effect of promoting artificial cultivation

Active Publication Date: 2019-10-08
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the currently published patents and publications do not menti

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  • Ophiocordyceps sinensis and its application
  • Ophiocordyceps sinensis and its application

Examples

Experimental program
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Example Embodiment

[0018] Example 1: Isolation and identification of Ophiocordyceps sinensis KD11-2

[0019] 1. Obtainment of KD11-2 strain: Fresh Cordyceps was collected from Yala Township, Lucheng Town, Kangding City, Ganzi Prefecture, Sichuan Province, and transported to our laboratory through cryopreservation. In a sterile operating room or ultra-clean workbench, remove the soil and plaque around the cordyceps, and disinfect the surface with 75% alcohol; use a sterile scalpel to remove the skin of the sclerotium and sub-seats, and remove the sclerotia and sub-seats. Partially cut into small pieces (be careful to avoid the intestines of the worms), and planted on PPDA solid plate medium, cultured at 9-16℃; the isolated and non-polluting typical colony of Cordyceps sinensis was confirmed to be Cordyceps sinensis after molecular identification , Get this strain, and number it as KD11-2.

[0020] 2. Features of KD11-2 strain:

[0021] Morphological characteristics: On the PPDA solid medium, the colon...

Example Embodiment

[0027] Example 2:

[0028] Experimental location: Guangdong Provincial Institute of Biological Resources Application in Guangzhou, the following cultures were all under normal oxygen concentration.

[0029] The KD11-2 strains of Trichosporum chinensis and other strains of Trichosporum chinensis (KD1223) were respectively connected to sterile solid PPDA medium. After 60 days of dark culture at 9°C, the colonies were picked and transferred to sterile liquid PPDA medium , At 9 ℃, 100rpm shaking culture (dark culture) for 65 days, select the same size of mycelial ball, diameter of 2-3 mm, and the light brown fermentation culture of the bacterial liquid as the liquid strain for the fruiting body of Cordyceps Cultivation and production.

[0030] In a sterile room or ultra-clean workbench, the liquid strains diluted 5 times with sterile water (30mL diluted liquid strains per 100g cultivation medium) are inserted into the sterile cultivation medium. Place the inoculated culture flask in th...

Example Embodiment

[0032] Example 3:

[0033] Experimental location: Guangdong Provincial Institute of Biological Resources Application in Guangzhou, the following cultures were all under normal oxygen concentration.

[0034] The T. chinensis KD11-2 strain and other T. chinensis strains (KD1223) were respectively connected to a sterile solid PPDA medium. After 45 days of dark culture at 16°C, the colonies were selected to be inoculated into the sterile liquid PPDA medium. At 16°C, 100 rpm shaking culture (dark culture) for 40 days, and the fermentation culture of the mycelial ball with uniform size and 2-3 mm in diameter was selected as the liquid strain for the cultivation and production of Cordyceps sinensis fruiting bodies.

[0035] In a sterile room or ultra-clean workbench, insert liquid strains diluted 10-fold with sterile water (30mL diluted liquid strains per 100g of cultivation medium) into the sterile cultivation medium. Place the inoculated culture flask in the dark at 13°C for 40 days. Aft...

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Abstract

The invention discloses a strain of Ophiocordyceps sinensis and its application. The Ophiocordyceps sinensis KD11-2 has a preservation number of GDMCC No: 60594. The Ophiocordyceps sinensis KD11-2 ofthe present invention can grow sporophore on an artificial medium and form ascocarp (ascospores), and has the outstanding ability to form ascospores, under artificial cultivation condition, the Ophiocordyceps sinensis KD11-2 is used to produce and collect Ophiocordyceps sinensis ascospores, and the artificial cultivation of the Ophiocordyceps sinensis sporophores can be promoted, which provides anew method for utilizing the obtained ascospores to infect the larvae of host Hepialus armoricanus of Cordyceps sinensis.

Description

Technical field: [0001] The invention belongs to the field of artificial cultivation of fruiting bodies of Cordyceps sinensis, and in particular relates to Ophiocordyceps sinensis KD11-2 which can grow fruiting bodies and form ascocarps (ascospores) on artificial culture medium and its application. Background technique: [0002] Cordyceps sinensis is the most distinctive biological resource in my country, belonging to Ascomycota, Sordariomycetes, Hypocreales, Ophiocordycipitaceae, and Ophiocordyceps. In Tibet, Qinghai, Yunnan, Sichuan, Gansu and other snow-capped alpine regions above 3,000 meters above sea level. [0003] Cordyceps sinensis is the larvae of the host insect, the bat moth, which is infected by Trichosporium fungus in China, and makes it ossified. Under suitable conditions, it grows to form a complex structure with worm (zombie) grass (fungi fruiting body). [0004] Cordyceps sinensis has many functions such as "tonifying the lungs, strengthening the kidneys, b...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01H15/00A01G18/40A01G18/20C12R1/645
CPCA01H15/00A01G18/20A01G18/40C12N3/00C12N1/145C12R2001/645
Inventor 曹莉韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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