Fertilization solution for in-vitro fertilization of cattle and use method thereof

A technology for insemination of semen and bovine body, applied in biochemical equipment and methods, embryo cells, microorganisms, etc., can solve the problems of low concentration, few fertilized eggs, and insufficient time, so as to ensure the quality of development, improve the development rate, and reduce production costs. Effect

Active Publication Date: 2019-10-08
NORTHWEST A & F UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the quality and quantity of embryos cultured in vitro are often inferior to embryos that develop normally in vivo. In bovine in vitro fertilization, cryopreserved semen is often used, which leads to unstable sperm quality and low concentration during in vitro fertilization. , leading to unstable fertilization rate of embryos, less fertilized eggs forming pronuclei, which in turn affects pronuclear fusion and cleavage, and affects early embryo development in vitro, which significantly restricts the wide application of this technology

Method used

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  • Fertilization solution for in-vitro fertilization of cattle and use method thereof
  • Fertilization solution for in-vitro fertilization of cattle and use method thereof

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Embodiment 1

[0023] (1) Source and preparation of reagents

[0024] Gentamicin, cephalosporin, inorganic salts, L-arginine, L-aspartic acid, L-carnitine, epinephrine, penicillamine, hypotaurine, heparin, paraffin oil (M8410) are sigma products;

[0025] The M199 basal culture medium without Hepes is a product of Thermo Company;

[0026] Extra-grade fetal bovine serum (FBS) is a product of Gibco;

[0027] Essential amino acids are products of Thermo Fisher (product number 1831512);

[0028] Non-essential amino acids are products of Thermo Fisher (product number 1958909);

[0029] Fatty acid-free bovine serum albumin EFAF-BSA is a product of sigma (product number A6003-5G);

[0030] Insulin-transferrin-selenium additive ITS-G is a product of Thermo Fisher (product number 1865342, insulin 1g / L, transferrin 0.55g / L, selenium 0.00067g / L);

[0031] β-mercaptoethanol is a product of Thermo Fisher (Product No. 1922445);

[0032] Hypotaurine is a product of sigma company (article number H1384...

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Abstract

The invention discloses a fertilization solution for in-vitro fertilization of cattle and a use method thereof. The fertilization solution includes a SOF basal medium without inositol, and also includes the following additives: L-arginine, L-aspartic acid, L-carnitine, adrenaline, penicillamine, hypotaurine, and heparin. The fertilization solution of the invention can effectively ensure the in-vitro fertilization rate of the bovine embryo and the development quality of the fertilized embryo.

Description

technical field [0001] The invention relates to the technical field of livestock embryo engineering, and more specifically relates to a fertilized liquid for bovine in vitro fertilization and a use method thereof. Background technique [0002] The in vitro culture of livestock embryos is a technology with potential research value and commercial value, which can be widely used in in vitro transplantation of embryos, animal gene editing and breeding, and the expansion of excellent livestock breeds. However, the quality and quantity of embryos cultured in vitro are often inferior to embryos that develop normally in vivo. In bovine in vitro fertilization, cryopreserved semen is often used, which leads to unstable sperm quality and low concentration during in vitro fertilization. , lead to unstable fertilization rate of embryos, less fertilized eggs forming pronuclei, which in turn affects pronuclear fusion and cleavage, and affects early embryo development in vitro, which signif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0604C12N2500/32C12N2500/33C12N2501/81C12N2501/91C12N2501/999
Inventor 张涌张景程王勇胜
Owner NORTHWEST A & F UNIV
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