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Blade position switching method, blade position switching device and ultrathin microtome comprising blade position switching device

A technology of switching devices and ultra-thin slices, which is applied in measuring devices, instruments, metal processing, etc., can solve the problems of sequence discontinuity and intermediate slice information loss, and achieve the effect of ensuring continuity

Active Publication Date: 2019-10-15
INST OF AUTOMATION CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned problems in the prior art, that is, in order to solve the problems of sequence discontinuity and intermediate slice information loss caused by cutting blade position switching, the first aspect of the embodiment of the present invention provides a blade position switching method for Ultramicrotome, the method comprises the steps of:

Method used

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  • Blade position switching method, blade position switching device and ultrathin microtome comprising blade position switching device
  • Blade position switching method, blade position switching device and ultrathin microtome comprising blade position switching device
  • Blade position switching method, blade position switching device and ultrathin microtome comprising blade position switching device

Examples

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example 1

[0068] The sample width (the length in the x-axis direction) is 500um, the single step is 250um, the number of continuous steps is 2 times, and the number of continuous slices is 4000.

[0069] Prepare the sample resin embedding block, diamond knife (cutting knife made of diamond) to refine the surface and exposed side, the size is 0.5x 0.8x 0.3mm (X x Y x Z), fixed on the sample holder and stably installed on the microtome sample arm. Finely align the sample face with the diamond knife edge, fill the diamond knife groove with water, and install the collector.

[0070] In the human-computer interaction interface of the ultra-thin slicer (the human-computer interaction interface that can be displayed on the display screen or the PLC panel, the human-computer interaction interface is connected with the signal connection of the blade position switching controller) to set the slice thickness to 50nm, slice window, slice Speed ​​and maximum number of slices on the cutting blade. ...

example 2

[0074] The thickness of the sample (the length along the x-axis) is 1.0mm, the single step is 400um, the number of continuous steps is 3 times, and the number of continuous slices is 6000.

[0075] Prepare the sample resin embedding block, diamond knife finishing surface and exposed side, the size is 1.0x 0.8x 0.5mm (X xY x Z), fixed on the sample holder and stably installed on the sample arm of the microtome. Finely align the sample face with the diamond knife edge, fill the diamond knife groove with water, and install the collector.

[0076] Set slice thickness to 30nm, slice window and slice speed in the microtome. The time interval for each slice is recorded and entered into the device control system.

[0077] When the number of slices is 4000 pieces, the maximum number of slices of the cutting blade at this part is reached, and the user enters the stepping direction (same as Example 1, no longer described) in the human-computer interaction interface (same as Example 1, n...

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Abstract

The embodiment of the invention relates to the technical field of biological tissue section preparation, in particular to a blade position switching method, a blade position switching device and an ultrathin microtome comprising the blade position switching device. According to the blade position switching method, the blade position switching device and the ultrathin microtome comprising the bladeposition switching device, the problems of sequence discontinuity, intermediate section information loss and the like caused when blade position of a slicer is switched. The blade position switchingmethod comprises the following steps: after the N1 section is sliced and when a sample arm moves until a sample is separated from the slicer, driving a blade of the slicer to step for the first time along the width direction of the sample; and after the first stepping and the M1,...the Mn sections are sliced, and when the sample arm moves until the sample is separated from the slicer, driving theblade of the slilcer to step along the width direction of the sample until the sum of all step amounts is equal to or greater than the width of the section. According to the he blade position switching method, the blade position switching device and the ultrathin microtome comprising the blade position switching device, the quality of the sections is guaranteed, so that the sequence of the sections is continuous, and the loss of intermediate section information is avoided.

Description

technical field [0001] The invention relates to the technical field of biological tissue slice preparation, in particular to a blade position switching method and device and an ultrathin slicer including the device. Background technique [0002] The serial ultrathin section scanning electron microscope imaging method (ssSEM, the English abbreviation of Serial Sections ScanningElectron Microscopy) is the method of using the ultrathin microtome of the reel design and the belt system combined with the scanning electron microscope to realize the automatic collection and image of serial ultrathin sections. Acquisition work, because this method can not only achieve 3D reconstruction of large-scale neural circuits, slice samples can be preserved, but also can be imaged in parallel by multiple electron microscopes, it has become the most effective solution recognized in the field of 3D reconstruction of large-volume bio-electron microscopes. The working principle of the ultramicroto...

Claims

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Application Information

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IPC IPC(8): B26D7/26B26D7/32G01N23/2202
CPCB26D7/2628B26D7/32G01N23/2202
Inventor 张丽娜马宏图李琳琳陈曦韩华
Owner INST OF AUTOMATION CHINESE ACAD OF SCI