Microfluidic chip for preventing cell occlusion by using sheath fluid countercurrent

Abstract

The invention discloses a microfluidic chip for preventing cell occlusion by using sheath fluid countercurrent. The microfluidic chip comprises a microfluidic chip main body, which has a focusing module, a sorting module and a main flow channel. The main flow channel is bifurcated at an outlet end of the microfluidic chip main body to form two liquid flow channels, a countercurrent sheath liquid flow path communicating with the main flow channel is disposed between the two flow channels, and the countercurrent sheath liquid flow path is provided with countercurrent sheath liquid opposite to the flow direction of the main flow channel. The microfluidic chip can solve the problem that the cells or particles adhere to a V-shaped wall surface in the prior art, which can prevent the flow channel from being blocked, improves the analysis or sorting precision, avoids the repeated removal and washing of the chip, and reduces the cost. The countercurrent sheath fluid of the present invention cooperates with the sheath fluid in the main flow channel to refocus a sample, and the cells stably flow out of the chip can be ensured.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to a microfluidic chip that prevents cell blockage by utilizing sheath liquid backflow. Background technique [0002] Microfluidic chip refers to a multifunctional integrated system for the analysis, detection, and sorting of microfluids and particles in biology and chemistry at the micron and submicron scales. Such an integrated system is called "lab on a chip". In such a tiny biological laboratory with only a few square centimeters, through relevant design, it can have the functions of sampling, sample pretreatment, reaction, separation and detection. Compared with conventional experimental operations, the use of microfluidic technology can reduce the number of reagents required for experiments, and realize the analysis and sorting of micron-scale particles. Advantages such as operation automation. [0003] Since the advent of microfluidic chips, they have received more and mo...

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Problems solved by technology

[0004] However, the outlet channel of a microfluidic chip applied to flow cell sorting generally includes at least one sorting channel and a waste liquid channel, so that a bulge relative to the direction of fluid flow will be formed at the intersection of the two outlet channels. The raised area forms a stagnation point in the flow. The existence of this stagnation point causes cells or particles to adhere to it, which in turn causes the aggregation of cells or particles, resulting in a change in the stable microfluidic flow state, which reduces the accuracy of analysis or sorting. At the same time It can also cause problems such as flow channel congestion, sample contamination, and purity decline.

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