Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for promoting growth of listeria monocytogenes

A technology for monocytogenes and Listeria, which is applied in the field of microbial inspection, can solve the problems affecting the inspection efficiency and long time of Listeria monocytogenes, and achieve the effects of shortening the cultivation time, promoting growth and improving work efficiency

Active Publication Date: 2019-10-18
北京市食品安全监控和风险评估中心(北京市食品检验所)
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the current national standard enrichment method, it takes a long time, which affects the detection efficiency of Listeria monocytogenes. Therefore, it is urgent to develop a method to promote the growth of Listeria monocytogenes in order to improve the detection efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for promoting growth of listeria monocytogenes
  • Method for promoting growth of listeria monocytogenes
  • Method for promoting growth of listeria monocytogenes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The promoting action of embodiment 1 yeast to the growth of Listeria monocytogenes

[0055] In this example, Saccharomyces cerevisiae ATCC 9763 is taken as an example to analyze the growth-promoting effect of yeast on Listeria monocytogenes. The specific method is as follows:

[0056] Aliquot LB1 culture based on 50mL sterilized centrifuge tubes, 20mL per tube. In order to verify the promoting effect of yeast on the growth of Listeria monocytogenes, the bacterial suspension of Listeria monocytogenes ATCC 19115 was serially diluted 10 times, and 10 -4 Listeria monocytogenes dilution of dilution gradient (the bacteria concentration of Listeria monocytogenes is 4×10 9 cfu / mL) for inoculation, wherein, the control group was only inoculated with 50 μL of Listeria monocytogenes bacterial suspension dilution, and the experimental group was inoculated with 100 μL of yeast suspension while inoculating 50 μL of Listeria monocytogenes bacterial suspension dilution. liquid (the c...

Embodiment 2

[0058] The effect of embodiment 2 dissolved oxygen conditions on the growth-promoting effect of yeast on Listeria monocytogenes

[0059] This embodiment analyzes whether oxygen affects the promotion effect of yeast on the growth of Listeria monocytogenes, and the specific method is as follows:

[0060] In a 50mL sterile centrifuge tube, divide 45mL of sterilized LB1 medium for subsequent use, and the control group is only inoculated with 50 μL of 100 μL prepared in Example 1. -4 The bacterial suspension dilution of Listeria monocytogenes ATCC 19115 in dilution gradient (the bacterial concentration of Listeria monocytogenes is 4×10 9 cfu / mL), the experimental group was inoculated with 50 μL of the bacterial suspension dilution of the above-mentioned Listeria monocytogenes ATCC 19115 and simultaneously inoculated with 100 μL of yeast suspension (the concentration of yeast bacteria was 6×10 12 cfu / mL). After inoculation, tighten the cap of the centrifuge tube, place the centrif...

Embodiment 3

[0062] Embodiment 3 rotation culture improves the promotion effect of yeast to the growth of Listeria monocytogenes

[0063] This embodiment analyzes the effect of rotary culture on the promotion of the growth of Listeria monocytogenes by yeast, and the specific methods are as follows:

[0064] In a 50mL aseptic centrifuge tube, divide 20mL of sterilized LB1 medium for subsequent use, and the control group is only inoculated with 50 μL of 100 μL prepared in Example 1. -4 Listeria monocytogenes ATCC 19115 bacterial suspension dilution of dilution gradient (the bacterial concentration of Listeria monocytogenes is 4×10 9 cfu / mL), the experimental group was inoculated with 100 μL of yeast suspension while inoculating 50 μL of the above Listeria monocytogenes suspension dilution (the concentration of yeast bacteria was 6×10 12 cfu / mL). According to five culture conditions of static culture, 10r / min, 20r / min, 40r / min, 60r / min at 37℃, the promotion effect of yeast on the growth of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of microbial detection, in particular to a method for promoting growth of listeria monocytogenes. According to the method, it is found that by means of saccharomycetes, the growth of the food-borne pathogenic bacterium, namely listeria monocytogenes, can be effectively promoted, the growth rate of listeria monocytogenes is increased, the time for enrichment culture is effectively shortened, and the culture and inspection work efficiency of listeria monocytogenes is improved. The saccharomycetes serve as a growth promoter and have general applicability to different listeria monocytogenes strains, and the method for promoting the growth of listeria monocytogenes lays a foundation for achieving rapid detection of listeria monocytogenes.

Description

technical field [0001] The invention relates to the technical field of microbial inspection, in particular to a method for promoting the growth of Listeria monocytogenes by using yeast and a growth promoter for Listeria monocytogenes containing yeast. Background technique [0002] Listeria monocytogenes (Listeria monocytogenes, referred to as Listeria monocytogenes) belongs to Gram-positive bacteria and is a common food-borne pathogen. The World Trade Organization listed it as a One of the four major foodborne pathogens. Listeria monocytogenes widely exists in nature, human infection can lead to meningitis, gastroenteritis, sepsis, miscarriage in pregnant women, etc. Newborns and those with low immunity are more susceptible to infection. The bacteria can tolerate freezing and drying, and can survive in extreme environments, posing a potential threat to human health. [0003] The current method for detecting Listeria monocytogenes is GB4789.30-2016. According to the requir...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12R1/01C12R1/865
CPCC12N1/20C12N1/38
Inventor 李树垚罗晓轩曹悦宋丽萍王丹武艳如毛婷崔旸毕思丹蔡雪凤穆同娜
Owner 北京市食品安全监控和风险评估中心(北京市食品检验所)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com