Method for quickly creating nucleocytoplasmic interaction sterile line

Abstract

The invention provides a method for quickly creating a nucleocytoplasmic interaction sterile line and relates to the technical field of breeding of agricultural seeds. The method comprises creating amale sterile line of a superior inbred line and quickly screening a corresponding maintainer line. An existing male sterile line is hybridized with the superior inbred line; a generation F1 and a maleparent material are inversely backcrossed; a sterile gene is introduced to a superior male parent; the male sterile line having agronomic traits consistent to those of the superior inbred line is created quickly through triple-generation breeding; the maintainer line is screened out, having sterile degree and sterility of 100%.

Description

technical field [0001] The invention belongs to the technical field of agricultural seed breeding, and in particular relates to a method for rapidly creating a nuclear-plasma interaction sterile line. Background technique [0002] Capsicum, as a vegetable crop with frequent cross-pollination, has obvious heterosis. The first generation of excellent hybrids can increase the yield by more than 50% compared with the general production species. It has been widely used in production and achieved obvious economic benefits. Pepper hybrid seed production is often artificially detasselled at the bud stage, which is labor-intensive and costly, and it is difficult to guarantee the purity of the seeds. [0003] The use of male sterile lines of peppers to produce hybrid varieties is an effective means to greatly reduce the cost of hybrid seed production and ensure the purity of seeds. When preparing hybrids, excellent parent materials have a greater impact on hybrid offspring. Therefore...

AI Technical Summary

Problems solved by technology

Generally, the male parent material with all-fertile offspring will be eliminated, because the male parent material does not carry the sterility gene, which will greatly miss the excellent parent material (maintainer line)

[0005] At the same time, when dealing with the separation of fertility or total sterility of hybrid offspring, especially how to transfer excellent parental materials into male sterile lines and screen maintainers, there is currently no quick and effective method. The traditional method of sterile line transfer , mainly using artificially synthesized maintainer lines, introducing the sterile gene into the male parent material through backcrossing, and then saturated backcrossing with the sterile line to continuously increase the proportion of the sterile gene, thereby obtaining a stable maintainer line

This method takes too long time, and the workload of selecting a combination with a high rate of sterile plants to backcross with the male parent for multiple generations is extremely heavy, and the male parent’s multi-generational self-crossing has a decline phenomenon, and it is very likely that the sterile rate and the male parent will not be transferred. CMS lines with high sterility, thus losing excellent maintainer lines

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