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Bacillus velezensis 118 and biological microbial inoculum and application thereof

A technology of Bacillus velesi and Bacillus, applied in the direction of biocide, application, biocide, etc., can solve the problem of unstable field effect of biocontrol agents, achieve high-efficiency biological control ability, promote bacterial swimming and Creeping, the effect of significant biocontrol effect

Inactive Publication Date: 2019-10-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Comparing the differences in biofilm morphology and yield, swimming and wriggling, and root adhesion between the wild strain and the mutant strain, and screening the corresponding high-efficiency genes, is expected to solve the technical bottleneck of the current unstable field effect of biocontrol agents

Method used

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  • Bacillus velezensis 118 and biological microbial inoculum and application thereof
  • Bacillus velezensis 118 and biological microbial inoculum and application thereof
  • Bacillus velezensis 118 and biological microbial inoculum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Screening and purification of 118 bacterial strains

[0048] (1) Primary screening

[0049] Take Pseudomonas solanacearum (Ralstonia solanacearum, Rs) CPG (0.1% peptone, 0.01% chelin amino acid, 0.05% glucose) culture solution (OD 600 ~0.4) 200 μL of bacterial liquid were put into sterile plates respectively, poured 20mL / CPG medium sterilized and cooled to about 45°C into the plate containing the bacterial liquid, put the plate in a horizontal position and quickly rotated the plate to make the medium Mix evenly with the bacterial solution, and after the culture medium is solidified, let it dry naturally to make a CPG plate containing Pseudomonas solanacearum for later use.

[0050] Take Guangzhou banana, tomato and other plant rhizosphere soil as the screening soil sample, weigh 10g and put it into a 250mL Erlenmeyer flask filled with 90mL sterile water (with glass beads), shake it on a shaker for 30min to fully disperse the bacteria, and let it stand for 20 ...

Embodiment 2118

[0055] Example 2 118 bacteria were identified as Bacillus velezensis

[0056] 118 bacteria are Gram-positive bacteria with spores, rod-shaped, aerobic, and motile; the colonies formed after culturing on LB solid medium for 24 hours are white and round, with a moist surface. The whole genome sequence of strain 118 was obtained by Illumina high-throughput sequencing and CLC gene splicing method, and the 16S rRNA sequence of strain 118 was obtained by comparing the 16S rRNA gene sequence by NCBI. The phylogenetic tree of 118 bacteria was constructed by the neighbor-joining method ( figure 2 ). The 16S rRNA sequence (SEQ ID NO: 1) of the strain of the present invention has high homology with the type strain Bacillus velezensis FZB42 in GenBank, and the similarity is 100%. Based on the results of phylogenetic tree construction based on plate colony characteristics and 16S rRNA sequence, the strain was preliminarily identified as Bacillus velezensis and named Bacillus velezensis1...

Embodiment 3118

[0057] Example 3 Gene sequences of 118 bacteria δX and ylaC factors

[0058] The whole genome sequence of strain 118 was obtained by Illumina high-throughput sequencing and CLC gene splicing method, and the 16SrRNA sequence and regulatory gene δ of strain 118 were obtained by comparing the gene sequences by NCBI X Sequence, as shown in SEQ ID NO.1, NO.2. The similarity between the sequence and the type strain Bacillus subtilis 3610 is 87%. The regulatory gene ylaC sequence of strain 118 was obtained, as shown in SEQ ID NO.3. The sequence is very different from the type strain Bacillus subtilis 3610, showing uniqueness.

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Abstract

The invention discloses bacillus velezensis (Bacillus velezensis) 118 and an application thereof. The preservation number of the strain is GDMCC No.60625, and the strain is preserved in the GuangdongMicrobiological Culture Collection Center (GDMCC) on April 4, 2019. The strain has the advantages of preventing and controlling tomato bacterial wilt and banana blight, promoting growth of plants, highly producing biomembranes, effectively colonizing rhizosphere and the like. The strain has 5 extracellular functional delta factors including delta M, delta W, delta X, delta V and ylaC, wherein a delta X gene has the functions of adjusting and controlling formation of creases and petals of biomembranes, increasing the yield of the biomembranes, increasing peristalsis and swimming speed and increasing the rhizosphere colonization rate, and a ylaC gene has the function of adjusting and controlling the shape of the biomembranes. The biological microbial inoculum is prepared through the strain,so that soil-borne diseases can be prevented and treated, the growth of the plants can be promoted, the application prospect is broad, and the bacillus velezensis belongs to the technical field of agriculture biology.

Description

technical field [0001] The invention relates to the field of biological control of plant soil-borne diseases, and relates to a microorganism and its application, in particular to a Bacillus velezensis 118 (Bacillus velezensis) and its application. Background technique [0002] Soil-borne diseases of crops are one of the main problems restricting yield and quality in agricultural production, such as fusarium wilt, bacterial wilt, blight, root rot and sheath blight, which commonly occur in southern China. No harvest. Banana wilt is a fungal soil-borne disease caused by Fusarium oxysporum f.sp.cubense, and tomato bacterial wilt is a bacterial disease caused by Ralstonia solanacearum. Soilborne diseases. Crop soil-borne diseases are mainly prevented and controlled through improved cultivation measures, physical disinfection, and chemical pesticides. However, the extensive use of chemical pesticides not only causes pesticide residues in crops, but also causes environmental poll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/07
CPCC12N1/20C12R2001/07C12N1/205A01N63/10
Inventor 蔡燕飞王玉琪章家恩刘晓玉周晗裴秦汉
Owner SOUTH CHINA AGRI UNIV
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