Prenylated flavonoid sinoflavonoid U, and preparation method and applications thereof
A technology for prenylation of flavonoids and peach seven, which is applied in the field of medicine and achieves the effects of easy operation of the preparation method, abundant raw materials, and remarkable economic and social benefits.
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Embodiment 1
[0016] The preparation method of the prenylated flavonoid phytoseptone U of the present invention is to take 9kg of the medicinal material of Lotus leaf lotus as a raw material, and extract 3 times with 18L of 95% ethanol by heating under reflux at a temperature of 95°C. The extraction time is 1.5 hours each time. The ethanol is recovered under reduced pressure to obtain the extract-like ethanol extract, which is suspended in 3.2L of distilled water, and extracted 3 times with petroleum ether, dichloromethane, ethyl acetate, and n-butanol. , 3.2L each time, time is 1.5 hours; the ethyl acetate extraction part is preliminarily separated by silica gel column chromatography, and the volume ratio is 100:0, 100:5, 100:7, 100:10, 100:30, 100 : 50, 100: 70, 100: 100, 100: 200, 0: 100 petroleum ether-acetone mixed solvent system for gradient elution, each gradient uses 13L eluent, the flow rate is 15mL / min, the volume of each 500ml is First aliquot, collect 260 fractions, each fraction...
Embodiment 2
[0018] The preparation method of a prenylated flavonoid compound Taoerheptone U of the present invention is to take 6kg of Lotus leaf medicinal material as raw material, and extract 3 times with 30L of 75% ethanol by heating under reflux at a temperature of 90°C. The extraction time is 2 hours each time. The ethanol is recovered under reduced pressure to obtain the extract-like ethanol extract, which is suspended in 2L of distilled water and extracted 3 times with petroleum ether, dichloromethane, ethyl acetate and n-butanol in sequence. 2L each time, the time is 2 hours; the ethyl acetate extraction part is preliminarily separated by silica gel column chromatography, and the volume ratio is 100:0, 100:5, 100:7, 100:10, 100:30, 100:50 , 100:70, 100:100, 100:200, 0:100 petroleum ether-acetone mixed solvent system for gradient elution, each gradient uses 9.1L of eluent, the flow rate is 10mL / min, and each 350ml volume is first-rate 260 fractions were collected, and each fraction ...
Embodiment 3
[0020] The preparation method of a prenyl flavonoid compound Taoerheptone U of the present invention is to take 8kg of the leaf lotus medicinal material as the raw material, use 24L, 85% by volume ethanol to heat and reflux for 3 times, and the extraction temperature is 92℃ The extraction time is 1.5 hours each time. The ethanol is recovered under reduced pressure to obtain the extract-like ethanol extract, which is suspended in 2.8L of distilled water and extracted with petroleum ether, dichloromethane, ethyl acetate and n-butanol for 3 times. , 2.8L each time, time is 1.5 hours; the ethyl acetate extraction part is preliminarily separated by silica gel column chromatography, and the volume ratio is 100:0, 100:5, 100:7, 100:10, 100:30, 100 : 50, 100: 70, 100: 100, 100: 200, 0: 100 petroleum ether-acetone mixed solvent system for gradient elution, each gradient uses 11.7L of eluent, the flow rate is 13mL / min, each 450ml volume As the first fraction, 260 fractions were collected...
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