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Fructosamine determination kit

A kit and fructosamine technology, applied in the field of clinical testing, can solve problems such as poor anti-interference and poor stability of reagents

Active Publication Date: 2019-11-01
HUBEI UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the NBT method for the determination of serum fructosamine has poor anti-interference, poor reagent stability and long-term precipitation of the substrate NBT. Therefore, this method needs to be improved. It is urgently needed to develop a stable and strong anti-interference kit for the determination of fructosamine.

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Embodiment Construction

[0024] Below in conjunction with specific embodiment, the present invention will be further described:

[0025] formula

[0026] R1 Trishydroxymethylaminomethane 100mmol / L

[0027] Sodium carbonate 50mmol / L

[0028] Triton 100 (TX-100) 5g / L

[0029] Sodium azide 1g / L

[0030] pH 10.8

[0031] R2 Glycine 5mmol / L

[0032] Ethylene glycol 100ml / L

[0033] Iodonitro blue tetrazolium chloride (INT) 1mmol / L

[0034] Sodium azide 1g / L

[0035] pH 6.0

[0036] Testing method

[0037] 1. Basic parameters

[0038] 1) Reaction type: two-point endpoint method 2) Temperature: 37°C

[0039] 3) Main wavelength: 546nm 4) Secondary wavelength: 660nm

[0040] 5) Sample volume: 20μl 6) Reagent 1 (R1) volume: 300μl

[0041] 7) Reagent 2 (R2) volume: 100μl 8) Reaction direction: liter reaction

[0042] 2. Determination

[0043] Sample: 20μl

[0044] see figure 2

[0045] 3. Calibration

[0046] Calibration method: This product adopts two-point calibration.

[0047] 4. Calculat...

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Abstract

The invention provides a fructosamine determination kit having the following formula of 100mmol / L of R1 trismetyl aminomethane, 50mmol / L of sodium carbonate, 5g / L of Triton100 (TX-100), 1g / L of sodiumazide and PH of 10.8. Through long-term experimental observation, the reagent is good in stability and can be placed for 18 months without precipitation by using an INT substrate.

Description

technical field [0001] The invention belongs to the technical field of clinical detection, and in particular relates to a fructosamine determination kit. Background technique [0002] The fructosamine determination kit is used to quantitatively determine the content of fructosamine in human serum in vitro; the determination of fructosamine (FRUCT) reflects the level of glycated serum protein, and can understand the average blood sugar level of patients in the past 1-2 weeks; it is not affected by temporary blood sugar The impact of concentration fluctuations is a research index for the long-term blood sugar control level of clinical diabetic patients. [0003] Serum glucose undergoes non-enzymatic glycosylation reaction with amino groups at the end of serum protein molecules to form a polymeric ketamine structure (fructosamine), which reduces nitro blue tetrazolium blue in an alkaline environment to form a dye. Colorimetric determination was carried out with glycosylated se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N21/78
CPCG01N33/66G01N21/78
Inventor 黄俊潮林宏帅焦铭
Owner HUBEI UNIV OF SCI & TECH
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